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- W2968709710 abstract "ABSTRACT Although Rad51 is the key protein in homologous recombination (HR), a major DNA double-strand break repair pathway, several auxiliary factors interact with Rad51 to promote productive HR. Here, we present an interdisciplinary characterization of the interaction between Rad51 and Swi5-Sfr1, a widely conserved auxiliary factor. NMR and site-specific crosslinking experiments revealed two distinct sites within the intrinsically disordered N-terminus of Sfr1 that cooperatively bind to Rad51. Although disruption of this binding severely impaired Rad51 stimulation in vitro, interaction mutants did not show any defects in DNA repair. Unexpectedly, in the absence of the Rad51 paralogs Rad55-Rad57, which constitute another auxiliary factor complex, these interaction mutants were unable to promote DNA repair. Our findings provide molecular insights into Rad51 stimulation by Swi5-Sfr1 and suggest that, rather than functioning in an independent subpathway of HR as was previously proposed, Rad55-Rad57 facilitates the recruitment of Swi5-Sfr1 to Rad51." @default.
- W2968709710 created "2019-08-22" @default.
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- W2968709710 date "2019-08-16" @default.
- W2968709710 modified "2023-10-16" @default.
- W2968709710 title "Rad51 Interaction Analysis Reveals a Functional Interplay Among Recombination Auxiliary Factors" @default.
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- W2968709710 doi "https://doi.org/10.1101/738179" @default.
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