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- W296874172 abstract "Grapevine leafroll disease can be caused by any of at least eight serologically distinct closteroviruses. They have been named Grapevine leafroll-associated viruses 1-8 (GLRaV-1 to -8). In State of Sao Paulo, only GLRaV-3 had been reported until now. With the availability of antisera against GLRaV-1, GLRaV-2 and GLRaV-3, serological tests were carried out to estimate the incidence of the three viruses. DAS-ELISA with antisera against GLRaV-1 and GLRaV-3, and F(ab ') 2 ELISA with antisera against GLRaV-2 and GLRaV-3, were used for testing the samples. Dormant cuttings and mixture of petioles and veins of basal leaves were used for antigen extraction. All infected plants, from 25 grapevine cultivars, were previously indexed by graft-inoculation to wood indicators. GLRaV-1, GLRaV-2 and GLRaV-3 were detected in 8.3%, 25.6% and 88.0% of 144, 160 and 192 samples tested, respectively. Double infection was found in 27 samples of 13 cultivars and triple infection was found in one single sample. Petioles and veins, in mixture, were better sources of antigen than scrapings of dormant cuttings. Healthy plants consistently gave negative results." @default.
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- W296874172 date "2002-08-16" @default.
- W296874172 modified "2023-09-26" @default.
- W296874172 title "Incidence of Grapevine leafroll-associated viruses 1, 2 and 3 on vineyards of Sao Paulo State, Brazil" @default.
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