Matches in SemOpenAlex for { <https://semopenalex.org/work/W2968804572> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2968804572 endingPage "29" @default.
- W2968804572 startingPage "17" @default.
- W2968804572 abstract "Microbiomes on Earth are often considered the most heterogeneous biological entities, but their vital roles in driving global biogeochemical cycles often remain elusive. DNA-based stable isotope probing (DNA-SIP) provides a powerful means to establish a direct link between biogeochemical processes and the taxonomic identities of active microorganisms involved in the processes. Combined with high-throughput sequencing, it significantly aids in deciphering ecophysiological functions of active microorganisms at the level of microbial communities. DNA-SIP relies solely on the propagation of targeted microbial communities, during which the entire genomes of daughter cells are synthesized and increasingly 13C-labeled. This growth on 13C-labeled substrate in association with cell division provides solid evidence for the functional importance and metabolic potential of targeted microorganisms. The essential prerequisite for a successful DNA-SIP experiment is the identification, with confidence, of isotopically enriched 13C-DNA, of which the amount is generally too low to allow for the direct measurement of 13C atomic percent of nucleic acid. The 13C labeling can be readily identified in the fractionated DNA by quantification of functional genes specific to the known targeted microorganisms, and by high-throughput sequencing of the total microbial communities via 16S rRNA genes without prior knowledge of which microorganisms are 13C-labeled (i.e., highly enriched in the heavy fractions relative to 12C (natural isotope abundance) control treatments). In this chapter, the protocol for obtaining DNA highly enriched in heavy isotope is presented using diazotrophic methanotrophs in a paddy soil as a case study." @default.
- W2968804572 created "2019-08-22" @default.
- W2968804572 creator A5002871247 @default.
- W2968804572 creator A5016376206 @default.
- W2968804572 creator A5085783045 @default.
- W2968804572 date "2019-01-01" @default.
- W2968804572 modified "2023-09-26" @default.
- W2968804572 title "DNA-Based Stable Isotope Probing" @default.
- W2968804572 cites W1513168266 @default.
- W2968804572 cites W1615978956 @default.
- W2968804572 cites W2040422637 @default.
- W2968804572 cites W2043120352 @default.
- W2968804572 cites W2064012286 @default.
- W2968804572 cites W2069969697 @default.
- W2968804572 cites W2070950316 @default.
- W2968804572 cites W2088711515 @default.
- W2968804572 cites W2104185722 @default.
- W2968804572 cites W2125177769 @default.
- W2968804572 cites W2130351033 @default.
- W2968804572 cites W2131535785 @default.
- W2968804572 cites W2150563641 @default.
- W2968804572 cites W2163568680 @default.
- W2968804572 doi "https://doi.org/10.1007/978-1-4939-9721-3_2" @default.
- W2968804572 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31407293" @default.
- W2968804572 hasPublicationYear "2019" @default.
- W2968804572 type Work @default.
- W2968804572 sameAs 2968804572 @default.
- W2968804572 citedByCount "7" @default.
- W2968804572 countsByYear W29688045722020 @default.
- W2968804572 countsByYear W29688045722022 @default.
- W2968804572 countsByYear W29688045722023 @default.
- W2968804572 crossrefType "book-chapter" @default.
- W2968804572 hasAuthorship W2968804572A5002871247 @default.
- W2968804572 hasAuthorship W2968804572A5016376206 @default.
- W2968804572 hasAuthorship W2968804572A5085783045 @default.
- W2968804572 hasConcept C104317684 @default.
- W2968804572 hasConcept C15151743 @default.
- W2968804572 hasConcept C175605896 @default.
- W2968804572 hasConcept C18903297 @default.
- W2968804572 hasConcept C2777624702 @default.
- W2968804572 hasConcept C51679486 @default.
- W2968804572 hasConcept C523546767 @default.
- W2968804572 hasConcept C54355233 @default.
- W2968804572 hasConcept C552990157 @default.
- W2968804572 hasConcept C70721500 @default.
- W2968804572 hasConcept C71915725 @default.
- W2968804572 hasConcept C86803240 @default.
- W2968804572 hasConceptScore W2968804572C104317684 @default.
- W2968804572 hasConceptScore W2968804572C15151743 @default.
- W2968804572 hasConceptScore W2968804572C175605896 @default.
- W2968804572 hasConceptScore W2968804572C18903297 @default.
- W2968804572 hasConceptScore W2968804572C2777624702 @default.
- W2968804572 hasConceptScore W2968804572C51679486 @default.
- W2968804572 hasConceptScore W2968804572C523546767 @default.
- W2968804572 hasConceptScore W2968804572C54355233 @default.
- W2968804572 hasConceptScore W2968804572C552990157 @default.
- W2968804572 hasConceptScore W2968804572C70721500 @default.
- W2968804572 hasConceptScore W2968804572C71915725 @default.
- W2968804572 hasConceptScore W2968804572C86803240 @default.
- W2968804572 hasLocation W29688045721 @default.
- W2968804572 hasLocation W29688045722 @default.
- W2968804572 hasOpenAccess W2968804572 @default.
- W2968804572 hasPrimaryLocation W29688045721 @default.
- W2968804572 hasRelatedWork W1576498690 @default.
- W2968804572 hasRelatedWork W2128153599 @default.
- W2968804572 hasRelatedWork W2163568680 @default.
- W2968804572 hasRelatedWork W2185169131 @default.
- W2968804572 hasRelatedWork W2383538682 @default.
- W2968804572 hasRelatedWork W2405050639 @default.
- W2968804572 hasRelatedWork W2968729274 @default.
- W2968804572 hasRelatedWork W3043668926 @default.
- W2968804572 hasRelatedWork W3209231359 @default.
- W2968804572 hasRelatedWork W4313486927 @default.
- W2968804572 isParatext "false" @default.
- W2968804572 isRetracted "false" @default.
- W2968804572 magId "2968804572" @default.
- W2968804572 workType "book-chapter" @default.