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• W2968875770 endingPage "14802" @default.
• W2968875770 startingPage "14776" @default.
• W2968875770 abstract "The free radical nitric oxide (NO•) exerts biological effects through the direct and reversible interaction with specific targets (e.g. soluble guanylate cyclase) or through the generation of secondary species, many of which can oxidize, nitrosate or nitrate biomolecules. The NO•-derived reactive species are typically short-lived, and their preferential fates depend on kinetic and compartmentalization aspects. Their detection and quantification are technically challenging. In general, the strategies employed are based either on the detection of relatively stable end products or on the use of synthetic probes, and they are not always selective for a particular species. In this study, we describe the biologically relevant characteristics of the reactive species formed downstream from NO•, and we discuss the approaches currently available for the analysis of NO•, nitrogen dioxide (NO2•), dinitrogen trioxide (N2O3), nitroxyl (HNO), and peroxynitrite (ONOO−/ONOOH), as well as peroxynitrite-derived hydroxyl (HO•) and carbonate anion (CO3•−) radicals. We also discuss the biological origins of and analytical tools for detecting nitrite (NO2−), nitrate (NO3−), nitrosyl–metal complexes, S-nitrosothiols, and 3-nitrotyrosine. Moreover, we highlight state–of–the–art methods, alert readers to caveats of widely used techniques, and encourage retirement of approaches that have been supplanted by more reliable and selective tools for detecting and measuring NO•-derived oxidants. We emphasize that the use of appropriate analytical methods needs to be strongly grounded in a chemical and biochemical understanding of the species and mechanistic pathways involved. The free radical nitric oxide (NO•) exerts biological effects through the direct and reversible interaction with specific targets (e.g. soluble guanylate cyclase) or through the generation of secondary species, many of which can oxidize, nitrosate or nitrate biomolecules. The NO•-derived reactive species are typically short-lived, and their preferential fates depend on kinetic and compartmentalization aspects. Their detection and quantification are technically challenging. In general, the strategies employed are based either on the detection of relatively stable end products or on the use of synthetic probes, and they are not always selective for a particular species. In this study, we describe the biologically relevant characteristics of the reactive species formed downstream from NO•, and we discuss the approaches currently available for the analysis of NO•, nitrogen dioxide (NO2•), dinitrogen trioxide (N2O3), nitroxyl (HNO), and peroxynitrite (ONOO−/ONOOH), as well as peroxynitrite-derived hydroxyl (HO•) and carbonate anion (CO3•−) radicals. We also discuss the biological origins of and analytical tools for detecting nitrite (NO2−), nitrate (NO3−), nitrosyl–metal complexes, S-nitrosothiols, and 3-nitrotyrosine. Moreover, we highlight state–of–the–art methods, alert readers to caveats of widely used techniques, and encourage retirement of approaches that have been supplanted by more reliable and selective tools for detecting and measuring NO•-derived oxidants. We emphasize that the use of appropriate analytical methods needs to be strongly grounded in a chemical and biochemical understanding of the species and mechanistic pathways involved." @default.
• W2968875770 created "2019-08-22" @default.
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• W2968875770 creator A5085479621 @default.
• W2968875770 date "2019-10-01" @default.
• W2968875770 modified "2023-10-14" @default.
• W2968875770 title "Detection and quantification of nitric oxide–derived oxidants in biological systems" @default.
• W2968875770 cites W133579554 @default.
• W2968875770 cites W1480892230 @default.
• W2968875770 cites W1484736436 @default.
• W2968875770 cites W1491694223 @default.
• W2968875770 cites W1493899800 @default.
• W2968875770 cites W1518703811 @default.
• W2968875770 cites W1519638308 @default.
• W2968875770 cites W1519943882 @default.
• W2968875770 cites W1521256352 @default.
• W2968875770 cites W1526947909 @default.
• W2968875770 cites W1541878890 @default.
• W2968875770 cites W1545423008 @default.
• W2968875770 cites W1545999232 @default.
• W2968875770 cites W1553889001 @default.
• W2968875770 cites W1569772723 @default.
• W2968875770 cites W1575458145 @default.
• W2968875770 cites W1583277079 @default.
• W2968875770 cites W1589360733 @default.
• W2968875770 cites W1602409208 @default.
• W2968875770 cites W16232142 @default.
• W2968875770 cites W1634873152 @default.
• W2968875770 cites W1832367618 @default.
• W2968875770 cites W1844185706 @default.
• W2968875770 cites W1868062960 @default.
• W2968875770 cites W1886833444 @default.
• W2968875770 cites W1895019051 @default.
• W2968875770 cites W1943182047 @default.
• W2968875770 cites W1963507120 @default.
• W2968875770 cites W1964058023 @default.
• W2968875770 cites W1964749557 @default.
• W2968875770 cites W1964829417 @default.
• W2968875770 cites W1965215617 @default.
• W2968875770 cites W1967162316 @default.
• W2968875770 cites W1967729440 @default.
• W2968875770 cites W1968033597 @default.
• W2968875770 cites W1969880500 @default.
• W2968875770 cites W1970155516 @default.
• W2968875770 cites W1970169137 @default.
• W2968875770 cites W1971643943 @default.
• W2968875770 cites W1972297823 @default.
• W2968875770 cites W1972989329 @default.
• W2968875770 cites W1973140371 @default.
• W2968875770 cites W1973258909 @default.
• W2968875770 cites W1974144765 @default.
• W2968875770 cites W1974256277 @default.
• W2968875770 cites W1974936925 @default.
• W2968875770 cites W1977338190 @default.
• W2968875770 cites W1977597523 @default.
• W2968875770 cites W1978109891 @default.
• W2968875770 cites W1978964795 @default.
• W2968875770 cites W1979659513 @default.
• W2968875770 cites W1979802917 @default.
• W2968875770 cites W1980210721 @default.
• W2968875770 cites W1980259297 @default.
• W2968875770 cites W1980449745 @default.
• W2968875770 cites W1980528903 @default.
• W2968875770 cites W1981120411 @default.
• W2968875770 cites W1981173052 @default.
• W2968875770 cites W1981716766 @default.
• W2968875770 cites W1982380290 @default.
• W2968875770 cites W1983426503 @default.
• W2968875770 cites W1983606357 @default.
• W2968875770 cites W1985177004 @default.
• W2968875770 cites W1985745419 @default.
• W2968875770 cites W1986386412 @default.
• W2968875770 cites W1987684904 @default.
• W2968875770 cites W1988163498 @default.
• W2968875770 cites W1988717330 @default.
• W2968875770 cites W1988841674 @default.
• W2968875770 cites W1989868403 @default.
• W2968875770 cites W1989980805 @default.
• W2968875770 cites W1990768518 @default.
• W2968875770 cites W1990802251 @default.
• W2968875770 cites W1990994084 @default.
• W2968875770 cites W1991282382 @default.
• W2968875770 cites W1991943765 @default.
• W2968875770 cites W1991952977 @default.
• W2968875770 cites W1993272766 @default.
• W2968875770 cites W1993616328 @default.
• W2968875770 cites W1996142411 @default.
• W2968875770 cites W1999457634 @default.
• W2968875770 cites W2002192155 @default.
• W2968875770 cites W2003060168 @default.
• W2968875770 cites W2003281598 @default.
• W2968875770 cites W2003683297 @default.
• W2968875770 cites W2003707324 @default.
• W2968875770 cites W2003985128 @default.

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