Matches in SemOpenAlex for { <https://semopenalex.org/work/W2968893295> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2968893295 endingPage "171" @default.
- W2968893295 startingPage "166" @default.
- W2968893295 abstract "Objective: To investigate the expression of LIM and SH3 protein 1 (LASP1) in renal cell carcinoma and its significance in the invasion and migration of renal clear cell carcinoma 786-O cell line. Methods: The expression level of LASP1 in 41 cases of renal cell carcinoma tissues and normal renal tissues was analyzed by immunohistochemistry. The relationship between the expression level of LASP1 and clinical characteristics was further analyzed. Expression of LASP1 in 10 cases of tumor tissues with or without lymph node metastasis was analyzed by Western blot. Furthermore, small interfering RNA (siRNA) targeting LASP1 was constructed and transfected into 786-O cells to downregulate LASP1 expression. The interference effect of LASP1 siRNA on LASP1 protein and the expression of related proteins in epithelial mesenchymal transition (EMT) pathway were detected by Western blot. The effects of LASP1 knockdown on cell proliferation, migration and invasion and gene expression were then assessed using CCK8 assay, transwell cell migration system and western blot analysis, respectively. Results: The positive rate of LASP1 expression in renal clear cell carcinoma tissues was 90.2% (37/41), which was significantly higher than that in the adjacent tissues (29.3%, P=0.002). The expression of LASP1 in renal cell carcinoma was positively correlated with lymph node metastasis and TNM stage of renal cell carcinoma (P<0.05). The results of Western blot showed that LASP1 (0.696±0.053) was highly expressed in renal cell carcinoma (1.459±0.628), especially in cases with lymph node metastasis (2.692±0.186, P<0.05). The LASP1 siRNA remarkably down-regulated the expression of LASP1 protein in 786-O cells. The abilities of proliferation, invasion and migration of 786-O cells were decreased significantly in the LASP1 siRNA groups.The relative expression of E-cadherin protein in the siRNA group (0.848±0.020) was significantly higher than those in the siRNA-NC group (0.671±0.018) and control group (0.691±0.037, P<0.05). The relative expression of N-cadherin protein in the siRNA group (0.449±0.047) was significantly lower than those in the siRNA-NC group (0.613±0.018) and control group (0.633±0.045, P<0.05). The relative expression of vimentin protein in the siRNA group (0.477±0.029) was significantly lower than those in the siRNA-NC group (0.598±0.069) and control group (0.633±0.045, P<0.05 for both). Conclusions: LASP1 is highly expressed in renal clear cell carcinoma, which is closely related to the development of the cancer. The effects of LASP1 on the invasion and migration of 786-O cells and lymph node metastasis may be related to the EMT.目的： 分析LIM和SH3蛋白1(LASP1)在肾癌组织中的表达及其与肾癌患者临床病理特征的关系，探讨沉默LASP1表达对肾透明细胞癌786－O细胞侵袭和迁移能力的影响。 方法： 应用免疫组织化学染色检测41例肾透明细胞癌患者肿瘤组织及其配对的癌旁组织中LASP1蛋白的表达水平，并分析LASP1蛋白表达与患者临床病理特征的关系。采用Western blot法检测10例伴或不伴淋巴结转移肾透明细胞癌组织中LASP1蛋白的表达差异。以小干扰RNA(siRNA)技术转染786－O细胞，采用Western blot法检测LASP1 siRNA对LASP1蛋白的干扰效果以及对上皮间质转化(EMT)通路中相关蛋白的表达影响。采用细胞计数盒8(CCK－8)法和Transwell实验检测下调LASP1蛋白表达对786－O细胞增殖、侵袭和迁移能力的影响。 结果： LASP1蛋白在肾透明细胞癌组织中的阳性表达率为90.2%(37/41)，明显高于其在癌旁组织中的阳性表达率(29.3%，12/41; P＝0.002)。LASP1在肾癌组织中的表达与肾癌的淋巴结转移和TNM分期呈正相关(均P<0.05)。Western blot法检测结果显示，LASP1蛋白在癌旁正常组织、无淋巴结转移肾癌组织和有淋巴结转移肾癌组织中的相对表达量分别为0.696±0.053、1.459±0.628和2.692±0.186，差异有统计学意义(P＝0.001)。沉默786－O细胞中LASP1蛋白的表达后，786－O细胞的增殖、侵袭和迁移能力均明显降低。LASP1 siRNA转染786－O细胞48 h后，siRNA转染组、阴性对照组和空白对照组786－O细胞中上皮表型标志物E－cadherin蛋白的相对表达量分别为0.848±0.020、0.671±0.018和0.691±0.037，siRNA转染组E－cadherin蛋白的表达明显增高(P<0.05)；siRNA转染组、阴性对照组和空白对照组786－O细胞中间质表型标志物N－cadherin蛋白的相对表达量分别为0.449±0.047、0.613±0.018和0.633±0.045，siRNA转染组N－cadherin蛋白的表达明显降低(P<0.05)；siRNA转染组、阴性对照组和空白对照组786－O细胞中间质表型标志物Vimentin蛋白的相对表达量分别为0.477±0.029、0.598±0.069和0.633±0.045，siRNA转染组Vimentin蛋白的表达明显降低(P<0.05)。 结论： LASP1蛋白在肾透明细胞癌中高表达，其高表达可能与肾癌的发生发展有关，并可能通过EMT调节肾癌细胞的侵袭和迁移能力，促进肿瘤的淋巴结转移。." @default.
- W2968893295 created "2019-08-22" @default.
- W2968893295 creator A5020221977 @default.
- W2968893295 creator A5024147822 @default.
- W2968893295 creator A5040027560 @default.
- W2968893295 creator A5074663362 @default.
- W2968893295 creator A5076500400 @default.
- W2968893295 creator A5090593493 @default.
- W2968893295 date "2017-03-23" @default.
- W2968893295 modified "2023-09-23" @default.
- W2968893295 title "[Expression of LIM and SH3 protein 1 in renal clear cell carcinoma and its effects on invasion and migration of renal clear cell carcinoma 786-O cells]." @default.
- W2968893295 doi "https://doi.org/10.3760/cma.j.issn.0253-3766.2017.03.002" @default.
- W2968893295 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28316213" @default.
- W2968893295 hasPublicationYear "2017" @default.
- W2968893295 type Work @default.
- W2968893295 sameAs 2968893295 @default.
- W2968893295 citedByCount "0" @default.
- W2968893295 crossrefType "journal-article" @default.
- W2968893295 hasAuthorship W2968893295A5020221977 @default.
- W2968893295 hasAuthorship W2968893295A5024147822 @default.
- W2968893295 hasAuthorship W2968893295A5040027560 @default.
- W2968893295 hasAuthorship W2968893295A5074663362 @default.
- W2968893295 hasAuthorship W2968893295A5076500400 @default.
- W2968893295 hasAuthorship W2968893295A5090593493 @default.
- W2968893295 hasConcept C104317684 @default.
- W2968893295 hasConcept C121608353 @default.
- W2968893295 hasConcept C126322002 @default.
- W2968893295 hasConcept C137738243 @default.
- W2968893295 hasConcept C142724271 @default.
- W2968893295 hasConcept C1491633281 @default.
- W2968893295 hasConcept C173396325 @default.
- W2968893295 hasConcept C2776415932 @default.
- W2968893295 hasConcept C2777472916 @default.
- W2968893295 hasConcept C2779013556 @default.
- W2968893295 hasConcept C502942594 @default.
- W2968893295 hasConcept C54009773 @default.
- W2968893295 hasConcept C54355233 @default.
- W2968893295 hasConcept C55493867 @default.
- W2968893295 hasConcept C62112901 @default.
- W2968893295 hasConcept C71924100 @default.
- W2968893295 hasConcept C76419328 @default.
- W2968893295 hasConcept C81885089 @default.
- W2968893295 hasConcept C86803240 @default.
- W2968893295 hasConceptScore W2968893295C104317684 @default.
- W2968893295 hasConceptScore W2968893295C121608353 @default.
- W2968893295 hasConceptScore W2968893295C126322002 @default.
- W2968893295 hasConceptScore W2968893295C137738243 @default.
- W2968893295 hasConceptScore W2968893295C142724271 @default.
- W2968893295 hasConceptScore W2968893295C1491633281 @default.
- W2968893295 hasConceptScore W2968893295C173396325 @default.
- W2968893295 hasConceptScore W2968893295C2776415932 @default.
- W2968893295 hasConceptScore W2968893295C2777472916 @default.
- W2968893295 hasConceptScore W2968893295C2779013556 @default.
- W2968893295 hasConceptScore W2968893295C502942594 @default.
- W2968893295 hasConceptScore W2968893295C54009773 @default.
- W2968893295 hasConceptScore W2968893295C54355233 @default.
- W2968893295 hasConceptScore W2968893295C55493867 @default.
- W2968893295 hasConceptScore W2968893295C62112901 @default.
- W2968893295 hasConceptScore W2968893295C71924100 @default.
- W2968893295 hasConceptScore W2968893295C76419328 @default.
- W2968893295 hasConceptScore W2968893295C81885089 @default.
- W2968893295 hasConceptScore W2968893295C86803240 @default.
- W2968893295 hasIssue "3" @default.
- W2968893295 hasLocation W29688932951 @default.
- W2968893295 hasOpenAccess W2968893295 @default.
- W2968893295 hasPrimaryLocation W29688932951 @default.
- W2968893295 hasRelatedWork W2140369224 @default.
- W2968893295 hasRelatedWork W2377659385 @default.
- W2968893295 hasRelatedWork W2562705514 @default.
- W2968893295 hasRelatedWork W2766649156 @default.
- W2968893295 hasRelatedWork W2993472225 @default.
- W2968893295 hasRelatedWork W3001922991 @default.
- W2968893295 hasRelatedWork W3004559441 @default.
- W2968893295 hasRelatedWork W3030073087 @default.
- W2968893295 hasRelatedWork W3195434531 @default.
- W2968893295 hasRelatedWork W4220720946 @default.
- W2968893295 hasVolume "39" @default.
- W2968893295 isParatext "false" @default.
- W2968893295 isRetracted "false" @default.
- W2968893295 magId "2968893295" @default.
- W2968893295 workType "article" @default.