Matches in SemOpenAlex for { <https://semopenalex.org/work/W2969073612> ?p ?o ?g. }
- W2969073612 endingPage "4743" @default.
- W2969073612 startingPage "4738" @default.
- W2969073612 abstract "Abstract The development of effective high‐throughput screening assays has contributed greatly to the wealth of designer enzymes available, by enabling rapid identification of desired variants from large mutant libraries. Here, we report a general and operationally simple end‐point assay for transaminases that enables the screening of both amine donors and acceptors in liquid phase. The spectrophotometric‐based screen exploits the amine donor 2‐aminoethylaniline (2‐AEA) and relies on reaction of in situ generated indole with Ehrlich's reagent. The assay has also been adapted to allow screening in the reverse direction by addition of indole and subsequent spectrophotometric analysis. Importantly, the screen provides qualitative information on the enantio‐preference of the individual biocatalysts. To increase the assay throughput, an engineered expression strain ( E. coli BL21(DE3) Δ tnaA ) lacking tryptophanase activity, was generated to enable reliable and direct evaluation of individual colonies arrayed on agar plates." @default.
- W2969073612 created "2019-08-22" @default.
- W2969073612 creator A5021582524 @default.
- W2969073612 creator A5056481585 @default.
- W2969073612 creator A5059886826 @default.
- W2969073612 creator A5073474169 @default.
- W2969073612 creator A5073510663 @default.
- W2969073612 date "2019-09-06" @default.
- W2969073612 modified "2023-09-27" @default.
- W2969073612 title "A Comprehensive Quantitative Assay for Amine Transaminases" @default.
- W2969073612 cites W1490719520 @default.
- W2969073612 cites W1578405462 @default.
- W2969073612 cites W1668340281 @default.
- W2969073612 cites W1753001848 @default.
- W2969073612 cites W1765414750 @default.
- W2969073612 cites W1969492379 @default.
- W2969073612 cites W1984180361 @default.
- W2969073612 cites W1986056153 @default.
- W2969073612 cites W1991624097 @default.
- W2969073612 cites W1994752630 @default.
- W2969073612 cites W1998263233 @default.
- W2969073612 cites W2009240916 @default.
- W2969073612 cites W2022621942 @default.
- W2969073612 cites W2024851405 @default.
- W2969073612 cites W2025856735 @default.
- W2969073612 cites W2036341269 @default.
- W2969073612 cites W2040851590 @default.
- W2969073612 cites W2041571635 @default.
- W2969073612 cites W2054397561 @default.
- W2969073612 cites W2087825380 @default.
- W2969073612 cites W2108742112 @default.
- W2969073612 cites W2124374902 @default.
- W2969073612 cites W2125957234 @default.
- W2969073612 cites W2135551767 @default.
- W2969073612 cites W2157535968 @default.
- W2969073612 cites W2192981192 @default.
- W2969073612 cites W2328445540 @default.
- W2969073612 cites W241392980 @default.
- W2969073612 cites W2438537421 @default.
- W2969073612 cites W2496645452 @default.
- W2969073612 cites W2519586082 @default.
- W2969073612 cites W2524638452 @default.
- W2969073612 cites W2528127298 @default.
- W2969073612 cites W2607108385 @default.
- W2969073612 cites W2623250387 @default.
- W2969073612 cites W2687141720 @default.
- W2969073612 cites W2765974006 @default.
- W2969073612 cites W2767029048 @default.
- W2969073612 cites W2767044445 @default.
- W2969073612 cites W2769903235 @default.
- W2969073612 cites W2782078732 @default.
- W2969073612 cites W2793209087 @default.
- W2969073612 cites W2875227727 @default.
- W2969073612 cites W2898223435 @default.
- W2969073612 cites W2944045445 @default.
- W2969073612 cites W4234557919 @default.
- W2969073612 cites W4237978388 @default.
- W2969073612 cites W4248515401 @default.
- W2969073612 cites W945384332 @default.
- W2969073612 doi "https://doi.org/10.1002/cctc.201901430" @default.
- W2969073612 hasPublicationYear "2019" @default.
- W2969073612 type Work @default.
- W2969073612 sameAs 2969073612 @default.
- W2969073612 citedByCount "6" @default.
- W2969073612 countsByYear W29690736122020 @default.
- W2969073612 countsByYear W29690736122021 @default.
- W2969073612 countsByYear W29690736122022 @default.
- W2969073612 countsByYear W29690736122023 @default.
- W2969073612 crossrefType "journal-article" @default.
- W2969073612 hasAuthorship W2969073612A5021582524 @default.
- W2969073612 hasAuthorship W2969073612A5056481585 @default.
- W2969073612 hasAuthorship W2969073612A5059886826 @default.
- W2969073612 hasAuthorship W2969073612A5073474169 @default.
- W2969073612 hasAuthorship W2969073612A5073510663 @default.
- W2969073612 hasBestOaLocation W29690736122 @default.
- W2969073612 hasConcept C104317684 @default.
- W2969073612 hasConcept C131779359 @default.
- W2969073612 hasConcept C143065580 @default.
- W2969073612 hasConcept C178790620 @default.
- W2969073612 hasConcept C185592680 @default.
- W2969073612 hasConcept C192527728 @default.
- W2969073612 hasConcept C21951064 @default.
- W2969073612 hasConcept C2779649380 @default.
- W2969073612 hasConcept C40875361 @default.
- W2969073612 hasConcept C43617362 @default.
- W2969073612 hasConcept C51323132 @default.
- W2969073612 hasConcept C547475151 @default.
- W2969073612 hasConcept C55493867 @default.
- W2969073612 hasConcept C70721500 @default.
- W2969073612 hasConcept C86803240 @default.
- W2969073612 hasConceptScore W2969073612C104317684 @default.
- W2969073612 hasConceptScore W2969073612C131779359 @default.
- W2969073612 hasConceptScore W2969073612C143065580 @default.
- W2969073612 hasConceptScore W2969073612C178790620 @default.
- W2969073612 hasConceptScore W2969073612C185592680 @default.
- W2969073612 hasConceptScore W2969073612C192527728 @default.
- W2969073612 hasConceptScore W2969073612C21951064 @default.
- W2969073612 hasConceptScore W2969073612C2779649380 @default.