FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2969100900> ?p ?o ?g. }

• W2969100900 endingPage "200" @default.
• W2969100900 startingPage "190" @default.
• W2969100900 abstract "To design an affinity ligand for purification of antigen-binding fragment (Fab) antibody, variable domain of heavy chain antibody (VHH) phage libraries were constructed from Fab-immunized Alpaca and subjected to biopanning against Fabs. To find the specific binders, we directly applied high-throughput sequencing (HTS) analysis of the VHH sequences in the panned phages on next-generation sequencer. The efficiently enriched sequences were aligned for construction of the phylogenetic tree to be categorized into five groups. VHHs from three major groups were first selected to analyze their properties as an affinity ligand. However, those VHHs were not suitable as an affinity ligand because of lack of resistance against alkaline pH and/or difficulty in acidic elution from the affinity column. So, we further searched the candidates from minor group sequences. Among five, one VHH showed the binding ability but with low affinity against Fabs. Therefore, we improved its affinity-by-affinity maturation through error-prone PCR library techniques. The final designed VHH showed highly alkaline pH resistance and easy acidic elution together with high affinity to Fabs. These results indicate that HTS techniques combined with biopanning and followed by error-prone PCR library techniques is powerful in designing specific binders with desired properties." @default.
• W2969100900 created "2019-08-22" @default.
• W2969100900 creator A5010403616 @default.
• W2969100900 creator A5015522711 @default.
• W2969100900 creator A5034689721 @default.
• W2969100900 creator A5043104576 @default.
• W2969100900 creator A5050634362 @default.
• W2969100900 creator A5075534059 @default.
• W2969100900 date "2019-10-01" @default.
• W2969100900 modified "2023-10-02" @default.
• W2969100900 title "Efficient Screening and Design of Variable Domain of Heavy Chain Antibody Ligands Through High Throughput Sequencing for Affinity Chromatography to Purify Fab Fragments" @default.
• W2969100900 cites W1485023117 @default.
• W2969100900 cites W1485948483 @default.
• W2969100900 cites W1580407891 @default.
• W2969100900 cites W1606431180 @default.
• W2969100900 cites W1689516906 @default.
• W2969100900 cites W1690893451 @default.
• W2969100900 cites W1900726394 @default.
• W2969100900 cites W1968091806 @default.
• W2969100900 cites W1977340060 @default.
• W2969100900 cites W1980260012 @default.
• W2969100900 cites W1984400438 @default.
• W2969100900 cites W1993575340 @default.
• W2969100900 cites W1993885632 @default.
• W2969100900 cites W1994323576 @default.
• W2969100900 cites W1998765242 @default.
• W2969100900 cites W2020368956 @default.
• W2969100900 cites W2023202529 @default.
• W2969100900 cites W2029386192 @default.
• W2969100900 cites W2034516179 @default.
• W2969100900 cites W2034989660 @default.
• W2969100900 cites W2035118667 @default.
• W2969100900 cites W2042344055 @default.
• W2969100900 cites W2043330120 @default.
• W2969100900 cites W2052444922 @default.
• W2969100900 cites W2070362125 @default.
• W2969100900 cites W2073940609 @default.
• W2969100900 cites W2074560222 @default.
• W2969100900 cites W2076582423 @default.
• W2969100900 cites W2084390276 @default.
• W2969100900 cites W2084556679 @default.
• W2969100900 cites W2094052190 @default.
• W2969100900 cites W2095553811 @default.
• W2969100900 cites W2097449851 @default.
• W2969100900 cites W2125915670 @default.
• W2969100900 cites W2139518419 @default.
• W2969100900 cites W2147451568 @default.
• W2969100900 cites W2148388239 @default.
• W2969100900 cites W2151737415 @default.
• W2969100900 cites W2165984662 @default.
• W2969100900 cites W2166103775 @default.
• W2969100900 cites W2169563920 @default.
• W2969100900 cites W2169610187 @default.
• W2969100900 cites W2729580563 @default.
• W2969100900 cites W2748772622 @default.
• W2969100900 cites W52917329 @default.
• W2969100900 doi "https://doi.org/10.1089/mab.2019.0027" @default.
• W2969100900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31411543" @default.
• W2969100900 hasPublicationYear "2019" @default.
• W2969100900 type Work @default.
• W2969100900 sameAs 2969100900 @default.
• W2969100900 citedByCount "6" @default.
• W2969100900 countsByYear W29691009002021 @default.
• W2969100900 countsByYear W29691009002022 @default.
• W2969100900 countsByYear W29691009002023 @default.
• W2969100900 crossrefType "journal-article" @default.
• W2969100900 hasAuthorship W2969100900A5010403616 @default.
• W2969100900 hasAuthorship W2969100900A5015522711 @default.
• W2969100900 hasAuthorship W2969100900A5034689721 @default.
• W2969100900 hasAuthorship W2969100900A5043104576 @default.
• W2969100900 hasAuthorship W2969100900A5050634362 @default.
• W2969100900 hasAuthorship W2969100900A5075534059 @default.
• W2969100900 hasConcept C104317684 @default.
• W2969100900 hasConcept C104428545 @default.
• W2969100900 hasConcept C116569031 @default.
• W2969100900 hasConcept C153911025 @default.
• W2969100900 hasConcept C159654299 @default.
• W2969100900 hasConcept C167625842 @default.
• W2969100900 hasConcept C170493617 @default.
• W2969100900 hasConcept C179247698 @default.
• W2969100900 hasConcept C181199279 @default.
• W2969100900 hasConcept C185592680 @default.
• W2969100900 hasConcept C186268636 @default.
• W2969100900 hasConcept C2776471321 @default.
• W2969100900 hasConcept C2781377902 @default.
• W2969100900 hasConcept C36394416 @default.
• W2969100900 hasConcept C40767141 @default.
• W2969100900 hasConcept C43617362 @default.
• W2969100900 hasConcept C54355233 @default.
• W2969100900 hasConcept C55493867 @default.
• W2969100900 hasConcept C70721500 @default.
• W2969100900 hasConcept C73826308 @default.
• W2969100900 hasConcept C86803240 @default.
• W2969100900 hasConceptScore W2969100900C104317684 @default.
• W2969100900 hasConceptScore W2969100900C104428545 @default.
• W2969100900 hasConceptScore W2969100900C116569031 @default.
• W2969100900 hasConceptScore W2969100900C153911025 @default.
• W2969100900 hasConceptScore W2969100900C159654299 @default.

• next