FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2969182408> ?p ?o ?g. }

• W2969182408 endingPage "720" @default.
• W2969182408 startingPage "708" @default.
• W2969182408 abstract "Streptomyces mobaraensis is a key player for the industrial production of the protein cross-linking enzyme microbial transglutaminase (MTG). Extra-cellular activation of MTG by the transglutaminase-activating metalloprotease (TAMP) is regulated by the TAMP inhibitory protein SSTI that belongs to the large Streptomyces subtilisin inhibitor (SSI) family. Despite decades of SSI research, the binding site for metalloproteases such as TAMP remained elusive in most of the SSI proteins. Moreover, SSTI is a MTG substrate, and the preferred glutamine residues for SSTI cross-linking are not determined. To address both issues, that is, determination of the TAMP and the MTG glutamine binding sites, SSTI was modified by distinct point mutations as well as elongation or truncation of the N-terminal peptide by six and three residues respectively. Structural integrity of the mutants was verified by the determination of protein melting points and supported by unimpaired subtilisin inhibitory activity. While exchange of single amino acids could not disrupt decisively the SSTI TAMP interaction, the N-terminally shortened variants clearly indicated the highly conserved Leu40-Tyr41 as binding motif for TAMP. Moreover, enzymatic biotinylation revealed that an adjacent glutamine pair, upstream from Leu40-Tyr41 in the SSTI precursor protein, is the preferred binding site of MTG. This extension peptide disturbs the interaction with TAMP. The structure of SSTI was furthermore determined by X-ray crystallography. While no structural data could be obtained for the N-terminal peptide due to flexibility, the core structure starting from Tyr41 could be determined and analysed, which superposes well with SSI-family proteins. ENZYMES: Chymotrypsin, EC3.4.21.1; griselysin (SGMPII, SgmA), EC3.4.24.27; snapalysin (ScNP), EC3.4.24.77; streptogrisin-A (SGPA), EC3.4.21.80; streptogrisin-B (SGPB), EC3.4.21.81; subtilisin BPN', EC3.4.21.62; transglutaminase, EC2.3.2.13; transglutaminase-activating metalloprotease (TAMP), EC3.4.-.-; tri-/tetrapeptidyl aminopeptidase, EC3.4.11.-; trypsin, EC3.4.21.4. DATABASES: The atomic coordinates and structure factors (PDB 6I0I) have been deposited in the Protein Data Bank (http://www.rcsb.org)." @default.
• W2969182408 created "2019-08-22" @default.
• W2969182408 creator A5007683465 @default.
• W2969182408 creator A5017865843 @default.
• W2969182408 creator A5044611582 @default.
• W2969182408 creator A5048040577 @default.
• W2969182408 creator A5060669653 @default.
• W2969182408 creator A5080754225 @default.
• W2969182408 creator A5087147814 @default.
• W2969182408 creator A5087830800 @default.
• W2969182408 date "2019-08-29" @default.
• W2969182408 modified "2023-10-13" @default.
• W2969182408 title "The N‐terminal peptide of the transglutaminase‐activating metalloprotease inhibitor from <i>Streptomyces mobaraensis</i> accommodates both inhibition and glutamine cross‐linking sites" @default.
• W2969182408 cites W1620264475 @default.
• W2969182408 cites W1663535880 @default.
• W2969182408 cites W1738190513 @default.
• W2969182408 cites W1755703704 @default.
• W2969182408 cites W1848488238 @default.
• W2969182408 cites W1900253885 @default.
• W2969182408 cites W1901168090 @default.
• W2969182408 cites W1964850285 @default.
• W2969182408 cites W1971989288 @default.
• W2969182408 cites W1983222240 @default.
• W2969182408 cites W1988987720 @default.
• W2969182408 cites W2003424893 @default.
• W2969182408 cites W2004112823 @default.
• W2969182408 cites W2012193516 @default.
• W2969182408 cites W2026780825 @default.
• W2969182408 cites W2035503835 @default.
• W2969182408 cites W2048930029 @default.
• W2969182408 cites W2074904307 @default.
• W2969182408 cites W2075600594 @default.
• W2969182408 cites W2084516020 @default.
• W2969182408 cites W2108921801 @default.
• W2969182408 cites W2124026197 @default.
• W2969182408 cites W2126337621 @default.
• W2969182408 cites W2129478737 @default.
• W2969182408 cites W2139958248 @default.
• W2969182408 cites W2150252622 @default.
• W2969182408 cites W2154197653 @default.
• W2969182408 cites W2163341755 @default.
• W2969182408 cites W2169701014 @default.
• W2969182408 cites W2210120548 @default.
• W2969182408 cites W2282726347 @default.
• W2969182408 cites W2284055954 @default.
• W2969182408 cites W2323777432 @default.
• W2969182408 cites W2505592290 @default.
• W2969182408 cites W2789492718 @default.
• W2969182408 cites W2810743279 @default.
• W2969182408 cites W2889107497 @default.
• W2969182408 cites W2896638259 @default.
• W2969182408 cites W4248872320 @default.
• W2969182408 doi "https://doi.org/10.1111/febs.15044" @default.
• W2969182408 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31420998" @default.
• W2969182408 hasPublicationYear "2019" @default.
• W2969182408 type Work @default.
• W2969182408 sameAs 2969182408 @default.
• W2969182408 citedByCount "5" @default.
• W2969182408 countsByYear W29691824082020 @default.
• W2969182408 countsByYear W29691824082021 @default.
• W2969182408 countsByYear W29691824082022 @default.
• W2969182408 crossrefType "journal-article" @default.
• W2969182408 hasAuthorship W2969182408A5007683465 @default.
• W2969182408 hasAuthorship W2969182408A5017865843 @default.
• W2969182408 hasAuthorship W2969182408A5044611582 @default.
• W2969182408 hasAuthorship W2969182408A5048040577 @default.
• W2969182408 hasAuthorship W2969182408A5060669653 @default.
• W2969182408 hasAuthorship W2969182408A5080754225 @default.
• W2969182408 hasAuthorship W2969182408A5087147814 @default.
• W2969182408 hasAuthorship W2969182408A5087830800 @default.
• W2969182408 hasBestOaLocation W29691824081 @default.
• W2969182408 hasConcept C104317684 @default.
• W2969182408 hasConcept C143065580 @default.
• W2969182408 hasConcept C167625842 @default.
• W2969182408 hasConcept C178498320 @default.
• W2969182408 hasConcept C181199279 @default.
• W2969182408 hasConcept C185592680 @default.
• W2969182408 hasConcept C2779281246 @default.
• W2969182408 hasConcept C2779349466 @default.
• W2969182408 hasConcept C2779811187 @default.
• W2969182408 hasConcept C515207424 @default.
• W2969182408 hasConcept C55493867 @default.
• W2969182408 hasConceptScore W2969182408C104317684 @default.
• W2969182408 hasConceptScore W2969182408C143065580 @default.
• W2969182408 hasConceptScore W2969182408C167625842 @default.
• W2969182408 hasConceptScore W2969182408C178498320 @default.
• W2969182408 hasConceptScore W2969182408C181199279 @default.
• W2969182408 hasConceptScore W2969182408C185592680 @default.
• W2969182408 hasConceptScore W2969182408C2779281246 @default.
• W2969182408 hasConceptScore W2969182408C2779349466 @default.
• W2969182408 hasConceptScore W2969182408C2779811187 @default.
• W2969182408 hasConceptScore W2969182408C515207424 @default.
• W2969182408 hasConceptScore W2969182408C55493867 @default.
• W2969182408 hasIssue "4" @default.
• W2969182408 hasLocation W29691824081 @default.
• W2969182408 hasLocation W29691824082 @default.
• W2969182408 hasLocation W29691824083 @default.
• W2969182408 hasOpenAccess W2969182408 @default.

• next