Matches in SemOpenAlex for { <https://semopenalex.org/work/W2969304897> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2969304897 endingPage "51" @default.
- W2969304897 startingPage "44" @default.
- W2969304897 abstract "Abstract Lipases cleaving oils into fatty acids and glycerol are of great interest for the use in increasing the efficiency of fuels. In this work, a novel lipase from Pseudomonas aeruginosa , P. aeruginosa A12, was isolated by ion-exchange and hydrophobic chromatography. The purity of lipase was shown by electrophoresis and its molecular weight was estimated to be ~ 31.6 kDa. The whole amino acid sequence was analyzed by an LC-MS/MS method. Temperature- and pH-dependent optimum of the enzyme compiled 30 °C and 7.5, respectively. The obtained enzyme exhibited 79 % similarity of amino acid sequence to a lipase isolated from the same strain of P. aeruginosa . Thus, the novel lipase was determined to belong to I.1 subfamily of bacterial true lipases. Three dimensional structure of the isolated lipase isoform was modeled based on obtained sequences. Amino acids forming the catalytic domain were shown in the model. Lid domain is suggested to be in the open conformation. These results provide a potential alternative for enzymatic digestion of fuel oils and serve for the development of fundamental knowledge of lipase activity." @default.
- W2969304897 created "2019-08-29" @default.
- W2969304897 creator A5006343067 @default.
- W2969304897 creator A5016243126 @default.
- W2969304897 creator A5058061296 @default.
- W2969304897 creator A5063751097 @default.
- W2969304897 creator A5070534310 @default.
- W2969304897 date "2019-06-01" @default.
- W2969304897 modified "2023-09-26" @default.
- W2969304897 title "Characterization of a novel lipase from Pseudomonas aeruginosa" @default.
- W2969304897 cites W1994455376 @default.
- W2969304897 cites W2017261831 @default.
- W2969304897 cites W2044936649 @default.
- W2969304897 cites W2049287712 @default.
- W2969304897 cites W2062526844 @default.
- W2969304897 cites W2068482259 @default.
- W2969304897 cites W2111537738 @default.
- W2969304897 cites W2611144344 @default.
- W2969304897 cites W4366730364 @default.
- W2969304897 doi "https://doi.org/10.2478/nbec-2019-0006" @default.
- W2969304897 hasPublicationYear "2019" @default.
- W2969304897 type Work @default.
- W2969304897 sameAs 2969304897 @default.
- W2969304897 citedByCount "1" @default.
- W2969304897 countsByYear W29693048972021 @default.
- W2969304897 crossrefType "journal-article" @default.
- W2969304897 hasAuthorship W2969304897A5006343067 @default.
- W2969304897 hasAuthorship W2969304897A5016243126 @default.
- W2969304897 hasAuthorship W2969304897A5058061296 @default.
- W2969304897 hasAuthorship W2969304897A5063751097 @default.
- W2969304897 hasAuthorship W2969304897A5070534310 @default.
- W2969304897 hasBestOaLocation W29693048971 @default.
- W2969304897 hasConcept C181199279 @default.
- W2969304897 hasConcept C185592680 @default.
- W2969304897 hasConcept C2779697368 @default.
- W2969304897 hasConcept C2780786110 @default.
- W2969304897 hasConcept C2780881558 @default.
- W2969304897 hasConcept C43617362 @default.
- W2969304897 hasConcept C515207424 @default.
- W2969304897 hasConcept C55493867 @default.
- W2969304897 hasConceptScore W2969304897C181199279 @default.
- W2969304897 hasConceptScore W2969304897C185592680 @default.
- W2969304897 hasConceptScore W2969304897C2779697368 @default.
- W2969304897 hasConceptScore W2969304897C2780786110 @default.
- W2969304897 hasConceptScore W2969304897C2780881558 @default.
- W2969304897 hasConceptScore W2969304897C43617362 @default.
- W2969304897 hasConceptScore W2969304897C515207424 @default.
- W2969304897 hasConceptScore W2969304897C55493867 @default.
- W2969304897 hasIssue "1" @default.
- W2969304897 hasLocation W29693048971 @default.
- W2969304897 hasOpenAccess W2969304897 @default.
- W2969304897 hasPrimaryLocation W29693048971 @default.
- W2969304897 hasRelatedWork W179057329 @default.
- W2969304897 hasRelatedWork W1984610692 @default.
- W2969304897 hasRelatedWork W2023598726 @default.
- W2969304897 hasRelatedWork W2035650518 @default.
- W2969304897 hasRelatedWork W2074200874 @default.
- W2969304897 hasRelatedWork W2093624914 @default.
- W2969304897 hasRelatedWork W2330967022 @default.
- W2969304897 hasRelatedWork W2807594297 @default.
- W2969304897 hasRelatedWork W2952690850 @default.
- W2969304897 hasRelatedWork W3119082144 @default.
- W2969304897 hasVolume "18" @default.
- W2969304897 isParatext "false" @default.
- W2969304897 isRetracted "false" @default.
- W2969304897 magId "2969304897" @default.
- W2969304897 workType "article" @default.