FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2969475194> ?p ?o ?g. }

• W2969475194 abstract "Polymerase chain reaction (PCR) has wide acceptance for rapid identification of pathogens and also for diagnosis of infectious conditions. However, because of economic and expertise constraints, a majority of small or peripheral laboratories do not use PCR. The objective of the present study was to develop a dry-reagent PCR assay as an alternative to conventional PCR to assess its applicability in routine laboratory practice using malB gene for identification of Escherichia coli as a model.A total of 184 isolates were selected for the study comprising clinical isolates of E. coli and non-E. coli including Shigella sp. and a few other control strains. The DNA was isolated from all the isolates. The isolated DNA as well as the overnight grown bacterial cultures were subjected to both conventional wet PCR and dry-reagent PCR.The genomic DNA isolated from E. coli showed amplification of malB gene in both conventional wet and dry-reagent PCR and the band was observed at 491 bp. In dry-reagent PCR, the overnight grown E. coli cells also showed positive result. The non-E. coli strains other than Shigella sp. showed negative in both conventional wet and dry-reagent PCR. Shigella sp. showed positive in both conventional wet and dry-reagent PCR.Considering the elimination of genomic DNA isolation step, and similar results with the conventional wet PCR, dry-reagent PCR may be a good alternative for the conventional wet PCR." @default.
• W2969475194 created "2019-08-29" @default.
• W2969475194 creator A5001399895 @default.
• W2969475194 creator A5006952450 @default.
• W2969475194 creator A5013232506 @default.
• W2969475194 creator A5033833412 @default.
• W2969475194 creator A5044309675 @default.
• W2969475194 creator A5051638817 @default.
• W2969475194 creator A5071921094 @default.
• W2969475194 creator A5078441201 @default.
• W2969475194 date "2019-01-01" @default.
• W2969475194 modified "2023-09-27" @default.
• W2969475194 title "A novel ready-to-use dry-reagent polymerase chain reaction for detection of Escherichia coli & Shigella species" @default.
• W2969475194 cites W1517480469 @default.
• W2969475194 cites W1970235149 @default.
• W2969475194 cites W1982240222 @default.
• W2969475194 cites W1990533491 @default.
• W2969475194 cites W2015510212 @default.
• W2969475194 cites W2019143437 @default.
• W2969475194 cites W2020013243 @default.
• W2969475194 cites W2029925900 @default.
• W2969475194 cites W2034767282 @default.
• W2969475194 cites W2091911562 @default.
• W2969475194 cites W2099661054 @default.
• W2969475194 cites W2107255366 @default.
• W2969475194 cites W2109987029 @default.
• W2969475194 cites W2111107538 @default.
• W2969475194 cites W2122662040 @default.
• W2969475194 cites W2123349167 @default.
• W2969475194 cites W2123657637 @default.
• W2969475194 cites W2134990479 @default.
• W2969475194 cites W2138115922 @default.
• W2969475194 cites W2141146278 @default.
• W2969475194 cites W2147509508 @default.
• W2969475194 cites W2153559181 @default.
• W2969475194 cites W2266895494 @default.
• W2969475194 cites W2286444285 @default.
• W2969475194 cites W2345450564 @default.
• W2969475194 cites W2413221206 @default.
• W2969475194 cites W2781573909 @default.
• W2969475194 doi "https://doi.org/10.4103/ijmr.ijmr_1394_17" @default.
• W2969475194 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6702690" @default.
• W2969475194 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31417036" @default.
• W2969475194 hasPublicationYear "2019" @default.
• W2969475194 type Work @default.
• W2969475194 sameAs 2969475194 @default.
• W2969475194 citedByCount "0" @default.
• W2969475194 crossrefType "journal-article" @default.
• W2969475194 hasAuthorship W2969475194A5001399895 @default.
• W2969475194 hasAuthorship W2969475194A5006952450 @default.
• W2969475194 hasAuthorship W2969475194A5013232506 @default.
• W2969475194 hasAuthorship W2969475194A5033833412 @default.
• W2969475194 hasAuthorship W2969475194A5044309675 @default.
• W2969475194 hasAuthorship W2969475194A5051638817 @default.
• W2969475194 hasAuthorship W2969475194A5071921094 @default.
• W2969475194 hasAuthorship W2969475194A5078441201 @default.
• W2969475194 hasBestOaLocation W29694751941 @default.
• W2969475194 hasConcept C104317684 @default.
• W2969475194 hasConcept C147789679 @default.
• W2969475194 hasConcept C153911025 @default.
• W2969475194 hasConcept C17757408 @default.
• W2969475194 hasConcept C185592680 @default.
• W2969475194 hasConcept C2776986154 @default.
• W2969475194 hasConcept C40875361 @default.
• W2969475194 hasConcept C49105822 @default.
• W2969475194 hasConcept C54355233 @default.
• W2969475194 hasConcept C547475151 @default.
• W2969475194 hasConcept C552990157 @default.
• W2969475194 hasConcept C86803240 @default.
• W2969475194 hasConcept C89423630 @default.
• W2969475194 hasConceptScore W2969475194C104317684 @default.
• W2969475194 hasConceptScore W2969475194C147789679 @default.
• W2969475194 hasConceptScore W2969475194C153911025 @default.
• W2969475194 hasConceptScore W2969475194C17757408 @default.
• W2969475194 hasConceptScore W2969475194C185592680 @default.
• W2969475194 hasConceptScore W2969475194C2776986154 @default.
• W2969475194 hasConceptScore W2969475194C40875361 @default.
• W2969475194 hasConceptScore W2969475194C49105822 @default.
• W2969475194 hasConceptScore W2969475194C54355233 @default.
• W2969475194 hasConceptScore W2969475194C547475151 @default.
• W2969475194 hasConceptScore W2969475194C552990157 @default.
• W2969475194 hasConceptScore W2969475194C86803240 @default.
• W2969475194 hasConceptScore W2969475194C89423630 @default.
• W2969475194 hasLocation W29694751941 @default.
• W2969475194 hasLocation W29694751942 @default.
• W2969475194 hasLocation W29694751943 @default.
• W2969475194 hasLocation W29694751944 @default.
• W2969475194 hasOpenAccess W2969475194 @default.
• W2969475194 hasPrimaryLocation W29694751941 @default.
• W2969475194 hasRelatedWork W1927358950 @default.
• W2969475194 hasRelatedWork W2014017011 @default.
• W2969475194 hasRelatedWork W2036347073 @default.
• W2969475194 hasRelatedWork W2059214036 @default.
• W2969475194 hasRelatedWork W2076614389 @default.
• W2969475194 hasRelatedWork W2121731497 @default.
• W2969475194 hasRelatedWork W2361972042 @default.
• W2969475194 hasRelatedWork W2955690304 @default.
• W2969475194 hasRelatedWork W3108873220 @default.
• W2969475194 hasRelatedWork W3211025746 @default.
• W2969475194 isParatext "false" @default.

• next