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- W2969543843 abstract "Molecular diagnostics have significantly advanced the early detection of diseases, where electrochemical sensing of biomarkers has shown considerable promise. For a nucleic acid-based electrochemical sensor with signal-off behavior, the performance is evaluated by percent signal suppression (% ss), which indicates the change in current after hybridization. The % ss is generally due to more redox molecules (e.g., methylene blue) associating with the probe DNA bases in the single-strand form than the double-strand form upon hybridization with the target nucleic acid. Nanostructured electrodes generally enhance electrochemical sensor performance via several mechanisms, including increased number of capture probes per electrode volume and unique nanoscale transport phenomena. Here, we employ nanoporous gold (np-Au) as a model electrode material to study the influence of probe immobilization solution concentration on sensor performance and the underlying mechanisms. Unlike planar gold (pl-Au) electrodes, where % ss reaches a steady state with increasing concentration of the grafting solution, the % ss displays peak performance at certain grafting solution concentrations followed by rapid deterioration and reversal of the % ss polarity, suggesting an unexpected case of increased charge transfer upon hybridization. Fluorometric assessments of electrochemically desorbed nucleic acids for different electrode morphologies reveal that a significant amount of DNA molecules (unhybridized and hybridized) remain within the nanopores posthybridization. Analysis of electrochemical signals (e.g., square wave voltammogram shape) suggests that the large unbound nucleic acid concentration may be altering the modes of methylene blue interaction with the nucleic acids and charge transfer to the electrode surfaces." @default.
- W2969543843 created "2019-08-29" @default.
- W2969543843 creator A5002724994 @default.
- W2969543843 creator A5066444182 @default.
- W2969543843 creator A5072752087 @default.
- W2969543843 date "2019-08-20" @default.
- W2969543843 modified "2023-10-06" @default.
- W2969543843 title "Anomalous Trends in Nucleic Acid-Based Electrochemical Biosensors with Nanoporous Gold Electrodes" @default.
- W2969543843 cites W135431704 @default.
- W2969543843 cites W1635618181 @default.
- W2969543843 cites W1883449737 @default.
- W2969543843 cites W1966220096 @default.
- W2969543843 cites W1974394336 @default.
- W2969543843 cites W1975431917 @default.
- W2969543843 cites W1976829642 @default.
- W2969543843 cites W1977600801 @default.
- W2969543843 cites W1991429069 @default.
- W2969543843 cites W1993927479 @default.
- W2969543843 cites W1995710632 @default.
- W2969543843 cites W2005533270 @default.
- W2969543843 cites W2008920638 @default.
- W2969543843 cites W2012003979 @default.
- W2969543843 cites W2013664130 @default.
- W2969543843 cites W2014567256 @default.
- W2969543843 cites W2023278126 @default.
- W2969543843 cites W2023786370 @default.
- W2969543843 cites W2025578218 @default.
- W2969543843 cites W2029934700 @default.
- W2969543843 cites W2034286870 @default.
- W2969543843 cites W2036282665 @default.
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- W2969543843 cites W2043468628 @default.
- W2969543843 cites W2046373134 @default.
- W2969543843 cites W2046457015 @default.
- W2969543843 cites W2048255163 @default.
- W2969543843 cites W2053188526 @default.
- W2969543843 cites W2054345707 @default.
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- W2969543843 cites W2106221863 @default.
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- W2969543843 cites W2123034204 @default.
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- W2969543843 cites W2272883134 @default.
- W2969543843 cites W2312265384 @default.
- W2969543843 cites W2315156931 @default.
- W2969543843 cites W2315806698 @default.
- W2969543843 cites W2320993116 @default.
- W2969543843 cites W2419379961 @default.
- W2969543843 cites W2465964901 @default.
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- W2969543843 cites W2550392351 @default.
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- W2969543843 doi "https://doi.org/10.1021/acs.analchem.9b02686" @default.
- W2969543843 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6751010" @default.
- W2969543843 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31429540" @default.
- W2969543843 hasPublicationYear "2019" @default.
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