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• W2969707598 abstract "In this article, novel epoxy resin-based hierarchical porous polymers (HPSs) have been prepared through a non-sol-gel and template-free approach using crystalline trimethylolpropane (TMP) as porogen. The polymers exhibit dimensional stability and possess 3-dimentional interconnected multi-scale pores. In range of 50 μm~10 nm are ultra-macro-pore in between skeleton, macro-pore on skeleton and meso-pore in network, respectively. The porosity and specific surface area can be adjusted in range of 91.2–82.5% and 225–156 m2/g, respectively. Using three kinds of hierarchical porous polymers as supports phospholipase D (PLD) was effectively immobilized through physical adsorption. Owing to high porosity of the support and improvement of mass transfer the loading amount of PLD reached as high as 223 mg/gsupport and the corresponding specific activity achieved up to 3.75 × 103 U/gsupport. Under optimized conditions and the phosphatidylserine (PS) yield reached 95.5% within 40 min at 45 °C. The immobilized PLD exhibited not only better storage stability and but also resistance to pH and thermal inactivation than free PLD. It was found that 73.5% of PS yield retained after 12 cycling reuses." @default.
• W2969707598 created "2019-08-29" @default.
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• W2969707598 date "2019-12-01" @default.
• W2969707598 modified "2023-09-30" @default.
• W2969707598 title "Efficient immobilization of phospholipase D on novel polymer supports with hierarchical pore structures" @default.
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• W2969707598 doi "https://doi.org/10.1016/j.ijbiomac.2019.08.192" @default.
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• W2969707598 hasPublicationYear "2019" @default.
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