FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2969738062> ?p ?o ?g. }

• W2969738062 endingPage "222" @default.
• W2969738062 startingPage "209" @default.
• W2969738062 abstract "CRISPR-based technologies have become central to genome engineering. However, CRISPR-based editing strategies are dependent on the repair of DNA breaks via endogenous DNA repair mechanisms, which increases susceptibility to unwanted mutations. Here we complement Cas9 with a recombinase's functionality by fusing a hyperactive mutant resolvase from transposon Tn3, a member of serine recombinases, to a catalytically inactive Cas9, which we term integrase Cas9 (iCas9). We demonstrate iCas9 targets DNA deletion and integration. First, we validate iCas9's function in Saccharomyces cerevisiae using a genome-integrated reporter. Cooperative targeting by CRISPR RNAs at spacings of 22 or 40 bp enables iCas9-mediated recombination. Next, iCas9's ability to target DNA deletion and integration in human HEK293 cells is demonstrated using dual GFP-mCherry fluorescent reporter plasmid systems. Finally, we show that iCas9 is capable of targeting integration into a genomic reporter locus. We envision targeting and design concepts of iCas9 will contribute to genome engineering and synthetic biology." @default.
• W2969738062 created "2019-08-29" @default.
• W2969738062 creator A5008377256 @default.
• W2969738062 creator A5008493660 @default.
• W2969738062 creator A5012658958 @default.
• W2969738062 creator A5026984704 @default.
• W2969738062 creator A5033262572 @default.
• W2969738062 creator A5078294528 @default.
• W2969738062 date "2019-08-01" @default.
• W2969738062 modified "2023-10-02" @default.
• W2969738062 title "RNA-Guided Recombinase-Cas9 Fusion Targets Genomic DNA Deletion and Integration" @default.
• W2969738062 cites W1514736121 @default.
• W2969738062 cites W1972255439 @default.
• W2969738062 cites W1977799538 @default.
• W2969738062 cites W1981160614 @default.
• W2969738062 cites W1981521195 @default.
• W2969738062 cites W1982378509 @default.
• W2969738062 cites W1985183405 @default.
• W2969738062 cites W1987587101 @default.
• W2969738062 cites W1990802904 @default.
• W2969738062 cites W1998893141 @default.
• W2969738062 cites W2003171404 @default.
• W2969738062 cites W2018148678 @default.
• W2969738062 cites W2019451098 @default.
• W2969738062 cites W2026871784 @default.
• W2969738062 cites W2027350316 @default.
• W2969738062 cites W2034694727 @default.
• W2969738062 cites W2039705224 @default.
• W2969738062 cites W2064815984 @default.
• W2969738062 cites W2067513453 @default.
• W2969738062 cites W2070055731 @default.
• W2969738062 cites W2077659966 @default.
• W2969738062 cites W2100303812 @default.
• W2969738062 cites W2107959600 @default.
• W2969738062 cites W2115420193 @default.
• W2969738062 cites W2116798329 @default.
• W2969738062 cites W2130912501 @default.
• W2969738062 cites W2138799439 @default.
• W2969738062 cites W2145202296 @default.
• W2969738062 cites W2165869860 @default.
• W2969738062 cites W2169333620 @default.
• W2969738062 cites W2202899490 @default.
• W2969738062 cites W2204786127 @default.
• W2969738062 cites W2240571864 @default.
• W2969738062 cites W2267596434 @default.
• W2969738062 cites W2286769477 @default.
• W2969738062 cites W2325480448 @default.
• W2969738062 cites W2336828812 @default.
• W2969738062 cites W2483673258 @default.
• W2969738062 cites W2514468066 @default.
• W2969738062 cites W2550079706 @default.
• W2969738062 cites W2581280362 @default.
• W2969738062 cites W2586212992 @default.
• W2969738062 cites W2588234461 @default.
• W2969738062 cites W2603648333 @default.
• W2969738062 cites W2751474071 @default.
• W2969738062 cites W2793309516 @default.
• W2969738062 cites W2806820493 @default.
• W2969738062 cites W2807198924 @default.
• W2969738062 cites W2883697525 @default.
• W2969738062 cites W2898448050 @default.
• W2969738062 cites W4211071958 @default.
• W2969738062 doi "https://doi.org/10.1089/crispr.2019.0013" @default.
• W2969738062 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6707420" @default.
• W2969738062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31436506" @default.
• W2969738062 hasPublicationYear "2019" @default.
• W2969738062 type Work @default.
• W2969738062 sameAs 2969738062 @default.
• W2969738062 citedByCount "12" @default.
• W2969738062 countsByYear W29697380622019 @default.
• W2969738062 countsByYear W29697380622020 @default.
• W2969738062 countsByYear W29697380622021 @default.
• W2969738062 countsByYear W29697380622022 @default.
• W2969738062 countsByYear W29697380622023 @default.
• W2969738062 crossrefType "journal-article" @default.
• W2969738062 hasAuthorship W2969738062A5008377256 @default.
• W2969738062 hasAuthorship W2969738062A5008493660 @default.
• W2969738062 hasAuthorship W2969738062A5012658958 @default.
• W2969738062 hasAuthorship W2969738062A5026984704 @default.
• W2969738062 hasAuthorship W2969738062A5033262572 @default.
• W2969738062 hasAuthorship W2969738062A5078294528 @default.
• W2969738062 hasBestOaLocation W29697380622 @default.
• W2969738062 hasConcept C102414288 @default.
• W2969738062 hasConcept C104317684 @default.
• W2969738062 hasConcept C132455925 @default.
• W2969738062 hasConcept C141231307 @default.
• W2969738062 hasConcept C142613039 @default.
• W2969738062 hasConcept C14430832 @default.
• W2969738062 hasConcept C144501496 @default.
• W2969738062 hasConcept C145290725 @default.
• W2969738062 hasConcept C156695909 @default.
• W2969738062 hasConcept C22744801 @default.
• W2969738062 hasConcept C2779260011 @default.
• W2969738062 hasConcept C54355233 @default.
• W2969738062 hasConcept C552990157 @default.
• W2969738062 hasConcept C70721500 @default.
• W2969738062 hasConcept C86803240 @default.
• W2969738062 hasConcept C98108389 @default.

• next