FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2969795893> ?p ?o ?g. }

• W2969795893 endingPage "69" @default.
• W2969795893 startingPage "61" @default.
• W2969795893 abstract "Abstract Localized aerosol delivery of gene therapies is a promising treatment of severe pulmonary diseases including lung cancer, cystic fibrosis, COPD and asthma. The administration of drugs by inhalation features multiple benefits including an enhanced patient acceptability and compliance. The application of a spray dried powder formulation has advantages over solutions due to their increased stability and shelf life. Furthermore, optimal sizes of the powder can be obtained by spray drying to allow a deep lung deposition. The present study optimized the parameters involved with spray drying polyplexes formed by polyethylenimine (PEI) and nucleic acids in inert excipients to generate a nano-embedded microparticle (NEM) powder with appropriate aerodynamic diameter. Furthermore, the effects of the excipient matrix used to generate the NEM powder on the biological activity of the nucleic acid and the ability to recover the embedded nanoparticles was investigated. The study showed that bioactivity and nucleic acid integrity was preserved after spray drying, and that polyplexes could be reconstituted from the dry powders made with trehalose but not mannitol as a stabilizer. Scanning electron microscopy (SEM) showed trehalose formulations that formed fused, lightly corrugated spherical particles in the range between 1 and 5 µm, while mannitol formulations had smooth surfaces and consisted of more defined particles. After redispersion of the microparticles in water, polyplex dispersions are obtained that are comparable to the initial formulations before spray drying. Cellular uptake and transfection studies conducted in lung adenocarcinoma cells show that redispersed trehalose particles performed similar to or better than polyplexes that were not spray dried. A method for quantifying polymer and nucleic acid loss following spray drying was developed in order to ensure that equal nucleic acid amounts were used in all in vitro experiments. The results confirm that spray dried NEM formulations containing nucleic acids can be prepared with characteristics known to be optimal for inhalation therapy." @default.
• W2969795893 created "2019-08-29" @default.
• W2969795893 creator A5001568662 @default.
• W2969795893 creator A5001878059 @default.
• W2969795893 creator A5025193205 @default.
• W2969795893 creator A5038636109 @default.
• W2969795893 creator A5041649444 @default.
• W2969795893 creator A5069727172 @default.
• W2969795893 date "2019-10-01" @default.
• W2969795893 modified "2023-10-12" @default.
• W2969795893 title "Characterization of spray dried powders with nucleic acid-containing PEI nanoparticles" @default.
• W2969795893 cites W1519480055 @default.
• W2969795893 cites W1965985576 @default.
• W2969795893 cites W1967252247 @default.
• W2969795893 cites W1971345094 @default.
• W2969795893 cites W1980613333 @default.
• W2969795893 cites W1984016003 @default.
• W2969795893 cites W1991290462 @default.
• W2969795893 cites W1994893251 @default.
• W2969795893 cites W2005271673 @default.
• W2969795893 cites W2016565975 @default.
• W2969795893 cites W2020718845 @default.
• W2969795893 cites W2023802920 @default.
• W2969795893 cites W2037842504 @default.
• W2969795893 cites W2046370810 @default.
• W2969795893 cites W2047983370 @default.
• W2969795893 cites W2054532423 @default.
• W2969795893 cites W2056690345 @default.
• W2969795893 cites W2058493200 @default.
• W2969795893 cites W2059564760 @default.
• W2969795893 cites W2085871110 @default.
• W2969795893 cites W2089129742 @default.
• W2969795893 cites W2095524329 @default.
• W2969795893 cites W2095549754 @default.
• W2969795893 cites W2110604072 @default.
• W2969795893 cites W2116424844 @default.
• W2969795893 cites W2120826143 @default.
• W2969795893 cites W2131283721 @default.
• W2969795893 cites W2140217618 @default.
• W2969795893 cites W2149381364 @default.
• W2969795893 cites W2156322778 @default.
• W2969795893 cites W2163792954 @default.
• W2969795893 cites W2167279371 @default.
• W2969795893 cites W2184277988 @default.
• W2969795893 cites W2297794140 @default.
• W2969795893 cites W2316898716 @default.
• W2969795893 cites W2317094391 @default.
• W2969795893 cites W2468043715 @default.
• W2969795893 cites W2585145241 @default.
• W2969795893 cites W2617905376 @default.
• W2969795893 cites W2741057361 @default.
• W2969795893 cites W2767402954 @default.
• W2969795893 cites W2791042850 @default.
• W2969795893 cites W2801301059 @default.
• W2969795893 doi "https://doi.org/10.1016/j.ejpb.2019.08.012" @default.
• W2969795893 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7611027" @default.
• W2969795893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31445157" @default.
• W2969795893 hasPublicationYear "2019" @default.
• W2969795893 type Work @default.
• W2969795893 sameAs 2969795893 @default.
• W2969795893 citedByCount "34" @default.
• W2969795893 countsByYear W29697958932019 @default.
• W2969795893 countsByYear W29697958932020 @default.
• W2969795893 countsByYear W29697958932021 @default.
• W2969795893 countsByYear W29697958932022 @default.
• W2969795893 countsByYear W29697958932023 @default.
• W2969795893 crossrefType "journal-article" @default.
• W2969795893 hasAuthorship W2969795893A5001568662 @default.
• W2969795893 hasAuthorship W2969795893A5001878059 @default.
• W2969795893 hasAuthorship W2969795893A5025193205 @default.
• W2969795893 hasAuthorship W2969795893A5038636109 @default.
• W2969795893 hasAuthorship W2969795893A5041649444 @default.
• W2969795893 hasAuthorship W2969795893A5069727172 @default.
• W2969795893 hasBestOaLocation W29697958932 @default.
• W2969795893 hasConcept C127413603 @default.
• W2969795893 hasConcept C155672457 @default.
• W2969795893 hasConcept C171250308 @default.
• W2969795893 hasConcept C185592680 @default.
• W2969795893 hasConcept C192562407 @default.
• W2969795893 hasConcept C24107716 @default.
• W2969795893 hasConcept C2777521759 @default.
• W2969795893 hasConcept C2779587474 @default.
• W2969795893 hasConcept C2780841128 @default.
• W2969795893 hasConcept C2984430287 @default.
• W2969795893 hasConcept C42360764 @default.
• W2969795893 hasConcept C43617362 @default.
• W2969795893 hasConcept C55493867 @default.
• W2969795893 hasConcept C77281830 @default.
• W2969795893 hasConceptScore W2969795893C127413603 @default.
• W2969795893 hasConceptScore W2969795893C155672457 @default.
• W2969795893 hasConceptScore W2969795893C171250308 @default.
• W2969795893 hasConceptScore W2969795893C185592680 @default.
• W2969795893 hasConceptScore W2969795893C192562407 @default.
• W2969795893 hasConceptScore W2969795893C24107716 @default.
• W2969795893 hasConceptScore W2969795893C2777521759 @default.
• W2969795893 hasConceptScore W2969795893C2779587474 @default.
• W2969795893 hasConceptScore W2969795893C2780841128 @default.
• W2969795893 hasConceptScore W2969795893C2984430287 @default.

• next