FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2969797506> ?p ?o ?g. }

• W2969797506 endingPage "110558" @default.
• W2969797506 startingPage "110558" @default.
• W2969797506 abstract "Freshwater (FW) fishes inhabit dilute environments and must actively absorb ions in order to counteract diffusive salt loss. Neuroendocrine control of ion uptake in FW fishes is an important feature of ion homeostasis and several important neuroendocrine factors have been identified. The role of serotonin (5-HT), however, has received less attention despite several studies pointing to a role for 5-HT in the control of ion balance. Here, we used a gene knockout approach to elucidate the role of 5-HT in regulating Na+ and Ca2+ uptake rates in larval zebrafish. Tryptophan hydroxylase (TPH) is the rate-limiting step in 5-HT synthesis and we therefore hypothesized that ion uptake rates would be altered in zebrafish larvae carrying knockout mutations in tph genes. We first examined the effect of tph1b knockout (KO) and found that tph1bKO larvae, obtained from Harvard University, had reduced rates of Na+ and Ca2+ uptake compared to wild-type (WT) larvae from our institution (uOttawa WT), lending support to our hypothesis. However, further experiments controlling for differences in genetic background demonstrated that WT larvae from Harvard University (Harvard WT) had lower ion uptake rates than those of uOttawa WT, and that ion uptake rate between Harvard WT and tph1bKO larvae were not significantly different. Therefore, our initial observation that tph1bKO larvae (Harvard source) had reduced ion uptake rates relative to uOttawa WT was a function of genetic background and not of knockout itself. These data provide a cautionary tale of the importance of controlling for genetic background in gene knockout experiments." @default.
• W2969797506 created "2019-08-29" @default.
• W2969797506 creator A5003027778 @default.
• W2969797506 creator A5004946930 @default.
• W2969797506 creator A5018990046 @default.
• W2969797506 creator A5019577502 @default.
• W2969797506 creator A5028844049 @default.
• W2969797506 creator A5036074139 @default.
• W2969797506 creator A5041716034 @default.
• W2969797506 creator A5071149333 @default.
• W2969797506 creator A5076327040 @default.
• W2969797506 date "2019-12-01" @default.
• W2969797506 modified "2023-09-27" @default.
• W2969797506 title "Use of gene knockout to examine serotonergic control of ion uptake in zebrafish reveals the importance of controlling for genetic background: A cautionary tale" @default.
• W2969797506 cites W1410930976 @default.
• W2969797506 cites W1965932856 @default.
• W2969797506 cites W1969998179 @default.
• W2969797506 cites W1973449157 @default.
• W2969797506 cites W1976391205 @default.
• W2969797506 cites W1977518722 @default.
• W2969797506 cites W1982708854 @default.
• W2969797506 cites W1994424017 @default.
• W2969797506 cites W1999906268 @default.
• W2969797506 cites W2001390487 @default.
• W2969797506 cites W2010403477 @default.
• W2969797506 cites W2015504752 @default.
• W2969797506 cites W2017224987 @default.
• W2969797506 cites W2028827586 @default.
• W2969797506 cites W2032655589 @default.
• W2969797506 cites W2034655287 @default.
• W2969797506 cites W2039031569 @default.
• W2969797506 cites W2052909713 @default.
• W2969797506 cites W2059682533 @default.
• W2969797506 cites W2060192747 @default.
• W2969797506 cites W2067376187 @default.
• W2969797506 cites W2068398839 @default.
• W2969797506 cites W2069298652 @default.
• W2969797506 cites W2069831820 @default.
• W2969797506 cites W2073565274 @default.
• W2969797506 cites W2088169443 @default.
• W2969797506 cites W2090442141 @default.
• W2969797506 cites W2101892854 @default.
• W2969797506 cites W2111034253 @default.
• W2969797506 cites W2120327277 @default.
• W2969797506 cites W2124177023 @default.
• W2969797506 cites W2126793993 @default.
• W2969797506 cites W2127342511 @default.
• W2969797506 cites W2134585047 @default.
• W2969797506 cites W2135050611 @default.
• W2969797506 cites W2135296770 @default.
• W2969797506 cites W2135827933 @default.
• W2969797506 cites W2156935972 @default.
• W2969797506 cites W2165534807 @default.
• W2969797506 cites W2192291434 @default.
• W2969797506 cites W2335279307 @default.
• W2969797506 cites W2368134136 @default.
• W2969797506 cites W2510939161 @default.
• W2969797506 cites W2615716853 @default.
• W2969797506 cites W2808042920 @default.
• W2969797506 cites W2921783490 @default.
• W2969797506 cites W3105702665 @default.
• W2969797506 cites W4244127391 @default.
• W2969797506 doi "https://doi.org/10.1016/j.cbpa.2019.110558" @default.
• W2969797506 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31446068" @default.
• W2969797506 hasPublicationYear "2019" @default.
• W2969797506 type Work @default.
• W2969797506 sameAs 2969797506 @default.
• W2969797506 citedByCount "4" @default.
• W2969797506 countsByYear W29697975062020 @default.
• W2969797506 countsByYear W29697975062021 @default.
• W2969797506 countsByYear W29697975062022 @default.
• W2969797506 countsByYear W29697975062023 @default.
• W2969797506 crossrefType "journal-article" @default.
• W2969797506 hasAuthorship W2969797506A5003027778 @default.
• W2969797506 hasAuthorship W2969797506A5004946930 @default.
• W2969797506 hasAuthorship W2969797506A5018990046 @default.
• W2969797506 hasAuthorship W2969797506A5019577502 @default.
• W2969797506 hasAuthorship W2969797506A5028844049 @default.
• W2969797506 hasAuthorship W2969797506A5036074139 @default.
• W2969797506 hasAuthorship W2969797506A5041716034 @default.
• W2969797506 hasAuthorship W2969797506A5071149333 @default.
• W2969797506 hasAuthorship W2969797506A5076327040 @default.
• W2969797506 hasConcept C104317684 @default.
• W2969797506 hasConcept C143065580 @default.
• W2969797506 hasConcept C170493617 @default.
• W2969797506 hasConcept C182704531 @default.
• W2969797506 hasConcept C207583985 @default.
• W2969797506 hasConcept C2775864247 @default.
• W2969797506 hasConcept C2775951788 @default.
• W2969797506 hasConcept C37000724 @default.
• W2969797506 hasConcept C41625074 @default.
• W2969797506 hasConcept C54355233 @default.
• W2969797506 hasConcept C77957584 @default.
• W2969797506 hasConcept C86803240 @default.
• W2969797506 hasConcept C99975950 @default.
• W2969797506 hasConceptScore W2969797506C104317684 @default.
• W2969797506 hasConceptScore W2969797506C143065580 @default.
• W2969797506 hasConceptScore W2969797506C170493617 @default.

• next