Matches in SemOpenAlex for { <https://semopenalex.org/work/W2969807776> ?p ?o ?g. }
- W2969807776 endingPage "14751" @default.
- W2969807776 startingPage "14742" @default.
- W2969807776 abstract "E2 conjugating enzymes are the key catalytic actors in the transfer of ubiquitin, SUMO, and other ubiquitin-like modifiers to their substrate proteins. Their high rates of transfer and promiscuous binding complicate studies of their interactions and binding partners. To access specific, covalently linked conjugates of the SUMO E2 conjugating enzyme Ubc9, we prepared synthetic variants bearing site-specific non-native modifications including the following: (1) replacement of Cys93 to 2,3-diaminopropionic acid to form the amide-linked stable E2–SUMO conjugate, which is known to have high affinity for E3 ligases; (2) a photoreactive group (diazirine) to trap E3 ligases upon UV irradiation; and (3) an N-terminal biotin for purification and detection. To construct these Ubc9 variants in a flexible, convergent manner, we combined the three leading methods: native chemical ligation (NCL), α-ketoacid–hydroxylamine (KAHA) ligation, and serine/threonine ligation (STL). Using the synthetic proteins, we demonstrated the selective formation of Ubc9–SUMO conjugates and the trapping of an E3 ligase (RanBP2) to form the stable, covalently linked SUMO1–Ubc9–RanBP2 ternary complex. The powerful combination of ligation methods—which minimizes challenges of functional group manipulations—will enable chemical probes based on E2 conjugating enzymes to trap E3 ligases and facilitate the synthesis of other protein classes." @default.
- W2969807776 created "2019-08-29" @default.
- W2969807776 creator A5011995716 @default.
- W2969807776 creator A5043586163 @default.
- W2969807776 creator A5049138769 @default.
- W2969807776 creator A5054453509 @default.
- W2969807776 creator A5058457451 @default.
- W2969807776 creator A5085934405 @default.
- W2969807776 date "2019-08-22" @default.
- W2969807776 modified "2023-10-14" @default.
- W2969807776 title "Chemical Synthesis of Atomically Tailored SUMO E2 Conjugating Enzymes for the Formation of Covalently Linked SUMO–E2–E3 Ligase Ternary Complexes" @default.
- W2969807776 cites W1579096605 @default.
- W2969807776 cites W1785257233 @default.
- W2969807776 cites W1995456120 @default.
- W2969807776 cites W2000439540 @default.
- W2969807776 cites W2004605701 @default.
- W2969807776 cites W2025686590 @default.
- W2969807776 cites W2040614735 @default.
- W2969807776 cites W2040825545 @default.
- W2969807776 cites W2043820375 @default.
- W2969807776 cites W2058489202 @default.
- W2969807776 cites W2058788882 @default.
- W2969807776 cites W2074344672 @default.
- W2969807776 cites W2077410458 @default.
- W2969807776 cites W2097299606 @default.
- W2969807776 cites W2105199969 @default.
- W2969807776 cites W2105289395 @default.
- W2969807776 cites W2108762552 @default.
- W2969807776 cites W2145069182 @default.
- W2969807776 cites W2145199752 @default.
- W2969807776 cites W2147542927 @default.
- W2969807776 cites W2155030186 @default.
- W2969807776 cites W2291388628 @default.
- W2969807776 cites W2330238097 @default.
- W2969807776 cites W2395256178 @default.
- W2969807776 cites W2400942913 @default.
- W2969807776 cites W2469376204 @default.
- W2969807776 cites W2484073039 @default.
- W2969807776 cites W2493385008 @default.
- W2969807776 cites W2520519686 @default.
- W2969807776 cites W2582931844 @default.
- W2969807776 cites W2585726420 @default.
- W2969807776 cites W2590813565 @default.
- W2969807776 cites W2596491515 @default.
- W2969807776 cites W2597003300 @default.
- W2969807776 cites W2601411499 @default.
- W2969807776 cites W2603600607 @default.
- W2969807776 cites W2606502180 @default.
- W2969807776 cites W2606698221 @default.
- W2969807776 cites W2610907738 @default.
- W2969807776 cites W2735492962 @default.
- W2969807776 cites W2746252656 @default.
- W2969807776 cites W2751492513 @default.
- W2969807776 cites W2751521307 @default.
- W2969807776 cites W2789882464 @default.
- W2969807776 cites W2795228462 @default.
- W2969807776 cites W2797773019 @default.
- W2969807776 cites W2800915293 @default.
- W2969807776 cites W2803389628 @default.
- W2969807776 cites W2818271651 @default.
- W2969807776 cites W2891413280 @default.
- W2969807776 cites W2892164726 @default.
- W2969807776 cites W2905279928 @default.
- W2969807776 cites W2913516597 @default.
- W2969807776 cites W2946238702 @default.
- W2969807776 cites W2948928505 @default.
- W2969807776 doi "https://doi.org/10.1021/jacs.9b06820" @default.
- W2969807776 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31436980" @default.
- W2969807776 hasPublicationYear "2019" @default.
- W2969807776 type Work @default.
- W2969807776 sameAs 2969807776 @default.
- W2969807776 citedByCount "33" @default.
- W2969807776 countsByYear W29698077762020 @default.
- W2969807776 countsByYear W29698077762021 @default.
- W2969807776 countsByYear W29698077762022 @default.
- W2969807776 countsByYear W29698077762023 @default.
- W2969807776 crossrefType "journal-article" @default.
- W2969807776 hasAuthorship W2969807776A5011995716 @default.
- W2969807776 hasAuthorship W2969807776A5043586163 @default.
- W2969807776 hasAuthorship W2969807776A5049138769 @default.
- W2969807776 hasAuthorship W2969807776A5054453509 @default.
- W2969807776 hasAuthorship W2969807776A5058457451 @default.
- W2969807776 hasAuthorship W2969807776A5085934405 @default.
- W2969807776 hasBestOaLocation W29698077762 @default.
- W2969807776 hasConcept C104317684 @default.
- W2969807776 hasConcept C134306372 @default.
- W2969807776 hasConcept C134459356 @default.
- W2969807776 hasConcept C171350616 @default.
- W2969807776 hasConcept C175114707 @default.
- W2969807776 hasConcept C178790620 @default.
- W2969807776 hasConcept C180577832 @default.
- W2969807776 hasConcept C181199279 @default.
- W2969807776 hasConcept C185592680 @default.
- W2969807776 hasConcept C197336794 @default.
- W2969807776 hasConcept C21951064 @default.
- W2969807776 hasConcept C25602115 @default.
- W2969807776 hasConcept C2778032875 @default.
- W2969807776 hasConcept C2779201268 @default.