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- W2969876614 abstract "We present three concepts for centrifugal microfluidics reducing ethanol carry-over in magnetic bead-based nucleic acid (NA) extraction. Ethanol carry-over is critical regarding inhibition of downstream NA amplification. We identified two possible carry-over pathways: Liquid co-transport within bead-clusters and vapor diffusion. For the first time, we integrated magnetic bead handling in centrifugal microfluidics at continuous rotation aiming to avoid liquid co-transport within bead-clusters. Consequently, no significant contribution to ethanol carry-over could be assigned anymore to liquid co-transport. Major carry-over was attributed to diffusive transport of ethanol vapor. Countermeasures reduced this from 9.7 % (v/v) to 0.4 % (v/v), below the critical level for inhibition of downstream amplification reactions." @default.
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- W2969876614 date "2019-06-01" @default.
- W2969876614 modified "2023-09-27" @default.
- W2969876614 title "Minimizing Ethanol Carry-Over in Centrifugal Microfluidic Nucleic Acid Extraction by Advanced Bead Handling and Management of Diffusive Mass Transfer" @default.
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- W2969876614 doi "https://doi.org/10.1109/transducers.2019.8808542" @default.
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