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- W2969883150 endingPage "420" @default.
- W2969883150 startingPage "403" @default.
- W2969883150 abstract "Human embryonic kidney 293 (HEK293) cells stably transfected with the rat P2X2 receptor subunit were preincubated with 200 nM progesterone (HEK293-P2X2-PROG), a potent positive allosteric modulator of homomeric P2X2 receptors, and used to detect low nanomolar concentrations of extracellular ATP. Fura-2-loaded HEK293-P2X2-PROG cells were acutely plated on top of cultured DH glial cells to quantify ATP release from single DH glial cells. Application of the α1 adrenoceptor agonist phenylephrine (PHE, 20 μM) or of a low K+ (0.2 mM) solution evoked reversible increases in the intracellular calcium concentration ([Ca2+]i) in the biosensor cells. A reversible increase in [Ca2+]i was also detected in half of the biosensor cells following the interruption of general extracellular perfusion. All increases in [Ca2+]i were blocked in the presence of the P2X2 antagonist PPADS or after preloading the glial cells with the calcium chelator BAPTA, indicating that they were due to calcium-dependent ATP release from the glial cells. ATP release induced by PHE was blocked by -l-phenylalanine 2-naphtylamide (GPN) that permeabilizes secretory lysosomes and bafilomycin A1 (Baf A1), an inhibitor of the H+-pump of acidic secretory vesicles. By contrast, ATP release induced by application of a low-K+ solution was abolished by Baf A1 but not by GPN. Finally, spontaneous ATP release observed after interrupting general perfusion was insensitive to both GPN and Baf A1 pretreatment. Our results indicate that ATP is released in a calcium-dependent manner from two distinct vesicular pools and one non-vesicular pool coexisting in DH glial cells and that noradrenaline and PHE selectively target the secretory lysosome pool." @default.
- W2969883150 created "2019-08-29" @default.
- W2969883150 creator A5004390340 @default.
- W2969883150 creator A5071552443 @default.
- W2969883150 creator A5072760094 @default.
- W2969883150 date "2019-08-23" @default.
- W2969883150 modified "2023-10-15" @default.
- W2969883150 title "High-resolution detection of ATP release from single cultured mouse dorsal horn spinal cord glial cells and its modulation by noradrenaline" @default.
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- W2969883150 doi "https://doi.org/10.1007/s11302-019-09673-2" @default.
- W2969883150 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6737151" @default.
- W2969883150 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31444738" @default.
- W2969883150 hasPublicationYear "2019" @default.