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- W2969883358 startingPage "941" @default.
- W2969883358 abstract "Human monocytes exposed to free arachidonic acid (AA), a secretory product of endothelial cells, acquire a foamy phenotype which is due to the accumulation of cytoplasmic lipid droplets with high AA content. Recruitment of foamy monocytes to the inflamed endothelium contributes to the development of atherosclerotic lesions. In this work, we investigated the potential role of AA stored in the neutral lipids of foamy monocytes to be cleaved by lipases and contribute to lipid mediator signaling. To this end, we used mass spectrometry-based lipidomic approaches combined with strategies to generate monocytes with different concentrations of AA. Results from our experiments indicate that the phospholipid AA pool in monocytes is stable and does not change upon exposure of the cells to the external AA. On the contrary, the AA pool in triacylglycerol is expandable and can accommodate relatively large amounts of fatty acid. Stimulation of the cells with opsonized zymosan results in the expected decreases of cellular AA. Under all conditions examined, all of the AA decreases observed in stimulated cells were accounted for by decreases in the phospholipid pool; we failed to detect any contribution of the triacylglycerol pool to the response. Experiments utilizing selective inhibitors of phospholipid or triacylglyerol hydrolysis confirmed that the phospholipid pool is the sole contributor of the AA liberated by stimulated cells. Thus, the AA in the triacylglycerol is not a source of free AA for the lipid mediator signaling during stimulation of human foamy monocytes and may be used for other cellular functions." @default.
- W2969883358 created "2019-08-29" @default.
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- W2969883358 date "2019-08-20" @default.
- W2969883358 modified "2023-09-27" @default.
- W2969883358 title "Neutral Lipids Are Not a Source of Arachidonic Acid for Lipid Mediator Signaling in Human Foamy Monocytes" @default.
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- W2969883358 cites W1587539418 @default.
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- W2969883358 cites W1646970599 @default.
- W2969883358 cites W1650090243 @default.
- W2969883358 cites W1661850314 @default.
- W2969883358 cites W1807832669 @default.
- W2969883358 cites W1870747190 @default.
- W2969883358 cites W1888402693 @default.
- W2969883358 cites W1965361015 @default.
- W2969883358 cites W1967327991 @default.
- W2969883358 cites W1975239118 @default.
- W2969883358 cites W1976282831 @default.
- W2969883358 cites W1987733994 @default.
- W2969883358 cites W2000081794 @default.
- W2969883358 cites W2000775162 @default.
- W2969883358 cites W2001327495 @default.
- W2969883358 cites W2003708730 @default.
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- W2969883358 cites W2020712150 @default.
- W2969883358 cites W2020885031 @default.
- W2969883358 cites W2025650515 @default.
- W2969883358 cites W2029317617 @default.
- W2969883358 cites W2034770942 @default.
- W2969883358 cites W2036604170 @default.
- W2969883358 cites W2037686566 @default.
- W2969883358 cites W2037708775 @default.
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- W2969883358 cites W2065026530 @default.
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- W2969883358 cites W2094576687 @default.
- W2969883358 cites W2097275712 @default.
- W2969883358 cites W2105753791 @default.
- W2969883358 cites W2106832927 @default.
- W2969883358 cites W2110135444 @default.
- W2969883358 cites W2113934014 @default.
- W2969883358 cites W2117307804 @default.
- W2969883358 cites W2124922239 @default.
- W2969883358 cites W2128635872 @default.
- W2969883358 cites W2131060092 @default.
- W2969883358 cites W2140861637 @default.
- W2969883358 cites W2141887017 @default.
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- W2969883358 cites W2147090946 @default.
- W2969883358 cites W2147620209 @default.
- W2969883358 cites W2152311269 @default.
- W2969883358 cites W2153111316 @default.
- W2969883358 cites W2156827480 @default.
- W2969883358 cites W2161402776 @default.
- W2969883358 cites W2172232070 @default.
- W2969883358 cites W2300552860 @default.
- W2969883358 cites W2315401917 @default.
- W2969883358 cites W2321683812 @default.
- W2969883358 cites W2325293303 @default.
- W2969883358 cites W2415501981 @default.
- W2969883358 cites W2417344553 @default.
- W2969883358 cites W2605391666 @default.
- W2969883358 cites W2759434971 @default.
- W2969883358 cites W2768498814 @default.
- W2969883358 cites W2771022510 @default.
- W2969883358 cites W2883180544 @default.
- W2969883358 cites W2886385224 @default.
- W2969883358 cites W2897779004 @default.
- W2969883358 cites W2903107073 @default.
- W2969883358 cites W2938210343 @default.
- W2969883358 cites W2945488840 @default.
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- W2969883358 cites W4248635034 @default.
- W2969883358 cites W4293247451 @default.
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- W2969883358 doi "https://doi.org/10.3390/cells8080941" @default.
- W2969883358 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6721759" @default.
- W2969883358 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31434356" @default.
- W2969883358 hasPublicationYear "2019" @default.
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