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- W2969927170 abstract "Abstract A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ 5 ‐3β‐hydroxysteroid dehydrogenase gene from Digitalis lanata , (b) a steroid Δ 5 ‐isomerase gene from Comamonas testosteronii , (c) a mutated steroid‐5β‐reductase gene from Arabidopsis thaliana, and (d) a steroid 21‐hydroxylase gene from Mus musculus . A second plasmid bearing an ADR/ADX fusion gene from Bos taurus was also constructed. A Saccharomyces cerevisiae strain bearing these two plasmids was generated. This strain, termed “CARD II yeast”, was capable of producing 5β‐pregnane‐3β,21‐diol‐20‐one, a central intermediate in 5β‐cardenolide biosynthesis, starting from pregnenolone which was added to the culture medium. Using this approach, five consecutive steps in cardenolide biosynthesis were realized in baker's yeast." @default.
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- W2969927170 date "2019-08-22" @default.
- W2969927170 modified "2023-10-05" @default.
- W2969927170 title "Biosynthetic approach to combine the first steps of cardenolide formation in <i>Saccharomyces cerevisiae</i>" @default.
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- W2969927170 doi "https://doi.org/10.1002/mbo3.925" @default.
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