FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2970119997> ?p ?o ?g. }

• W2970119997 endingPage "104769" @default.
• W2970119997 startingPage "104769" @default.
• W2970119997 abstract "Phialophora malorum is a well-known copper tolerant fungus causing soft rot decay of copper-treated wood in-service in ground contact situations worldwide. The aim of the present work was to develop a method that allowed studies of the mechanism(s) behind extracellular copper tolerance by examining the profile of proteins expressed by P. malorum in the cell wall/slime (subproteome) environment in mycelia grown in Cu-supplemented media. The study involves development of a novel non-destructive approach using mild extraction, concentration, separation (SDS-PAGE electrophoresis) and characterization (MALDI-TOF MS/MS) of the proteins present. Studies revealed differential protein profiles and expression in Cu-supplemented (0.1% CuSO4) vs control shake cultures after 10 days growth. Twelve proteins including several hydrolytic (i.e. glucosidases, pectinase, polygalacturonase) enzymes were either unique or over-expressed in Cu-cultures compared to controls. The cell wall location of the proteins was consistent with a role in copper tolerance and detoxification by binding Cu-ions extracellularly. Several of the proteins (e.g. hydrolases, pectinase) are also involved in wood cell wall biomineralization. It is proposed that the concentration of metabolites (organic acids) slowly released in close vicinity of hyphal surfaces by weakly bound enzymes over-expressed through Cu-stress may function to reduce levels of free non-chelated Cu (II) ions entering the cell cytoplasm and therefore provide a first level of defense against copper toxicity." @default.
• W2970119997 created "2019-09-05" @default.
• W2970119997 creator A5010381132 @default.
• W2970119997 creator A5012499022 @default.
• W2970119997 creator A5016187847 @default.
• W2970119997 creator A5052560966 @default.
• W2970119997 creator A5070691653 @default.
• W2970119997 date "2019-10-01" @default.
• W2970119997 modified "2023-10-16" @default.
• W2970119997 title "Method for characterizing extracellular proteins from the cell wall proteome of the copper tolerant fungus Phialophora malorum" @default.
• W2970119997 cites W1792134045 @default.
• W2970119997 cites W190293211 @default.
• W2970119997 cites W1965894780 @default.
• W2970119997 cites W1972622843 @default.
• W2970119997 cites W1975308046 @default.
• W2970119997 cites W1981114787 @default.
• W2970119997 cites W1981172857 @default.
• W2970119997 cites W1990006358 @default.
• W2970119997 cites W1990774637 @default.
• W2970119997 cites W1995683177 @default.
• W2970119997 cites W2008745098 @default.
• W2970119997 cites W2008773282 @default.
• W2970119997 cites W2018947207 @default.
• W2970119997 cites W2021488144 @default.
• W2970119997 cites W2027683710 @default.
• W2970119997 cites W2032811212 @default.
• W2970119997 cites W2036180776 @default.
• W2970119997 cites W2037422437 @default.
• W2970119997 cites W2038445054 @default.
• W2970119997 cites W2043287753 @default.
• W2970119997 cites W2051583593 @default.
• W2970119997 cites W2058634181 @default.
• W2970119997 cites W2066318277 @default.
• W2970119997 cites W2075070271 @default.
• W2970119997 cites W2085525174 @default.
• W2970119997 cites W2095140551 @default.
• W2970119997 cites W2103000070 @default.
• W2970119997 cites W2108791511 @default.
• W2970119997 cites W2113902494 @default.
• W2970119997 cites W2123153288 @default.
• W2970119997 cites W2135541075 @default.
• W2970119997 cites W2137235961 @default.
• W2970119997 cites W2137913263 @default.
• W2970119997 cites W2138133257 @default.
• W2970119997 cites W2153764774 @default.
• W2970119997 cites W2161109813 @default.
• W2970119997 cites W2263345929 @default.
• W2970119997 cites W2556177297 @default.
• W2970119997 cites W2564158620 @default.
• W2970119997 cites W2737487120 @default.
• W2970119997 cites W2883103220 @default.
• W2970119997 cites W2903777907 @default.
• W2970119997 cites W2952021899 @default.
• W2970119997 cites W434721590 @default.
• W2970119997 doi "https://doi.org/10.1016/j.ibiod.2019.104769" @default.
• W2970119997 hasPublicationYear "2019" @default.
• W2970119997 type Work @default.
• W2970119997 sameAs 2970119997 @default.
• W2970119997 citedByCount "4" @default.
• W2970119997 countsByYear W29701199972020 @default.
• W2970119997 countsByYear W29701199972022 @default.
• W2970119997 countsByYear W29701199972023 @default.
• W2970119997 crossrefType "journal-article" @default.
• W2970119997 hasAuthorship W2970119997A5010381132 @default.
• W2970119997 hasAuthorship W2970119997A5012499022 @default.
• W2970119997 hasAuthorship W2970119997A5016187847 @default.
• W2970119997 hasAuthorship W2970119997A5052560966 @default.
• W2970119997 hasAuthorship W2970119997A5070691653 @default.
• W2970119997 hasBestOaLocation W29701199972 @default.
• W2970119997 hasConcept C104397665 @default.
• W2970119997 hasConcept C125235067 @default.
• W2970119997 hasConcept C172854840 @default.
• W2970119997 hasConcept C178790620 @default.
• W2970119997 hasConcept C181199279 @default.
• W2970119997 hasConcept C185592680 @default.
• W2970119997 hasConcept C190062978 @default.
• W2970119997 hasConcept C28406088 @default.
• W2970119997 hasConcept C544778455 @default.
• W2970119997 hasConcept C55493867 @default.
• W2970119997 hasConcept C86803240 @default.
• W2970119997 hasConcept C89423630 @default.
• W2970119997 hasConceptScore W2970119997C104397665 @default.
• W2970119997 hasConceptScore W2970119997C125235067 @default.
• W2970119997 hasConceptScore W2970119997C172854840 @default.
• W2970119997 hasConceptScore W2970119997C178790620 @default.
• W2970119997 hasConceptScore W2970119997C181199279 @default.
• W2970119997 hasConceptScore W2970119997C185592680 @default.
• W2970119997 hasConceptScore W2970119997C190062978 @default.
• W2970119997 hasConceptScore W2970119997C28406088 @default.
• W2970119997 hasConceptScore W2970119997C544778455 @default.
• W2970119997 hasConceptScore W2970119997C55493867 @default.
• W2970119997 hasConceptScore W2970119997C86803240 @default.
• W2970119997 hasConceptScore W2970119997C89423630 @default.
• W2970119997 hasFunder F4320321033 @default.
• W2970119997 hasLocation W29701199971 @default.
• W2970119997 hasLocation W29701199972 @default.
• W2970119997 hasOpenAccess W2970119997 @default.
• W2970119997 hasPrimaryLocation W29701199971 @default.

• next