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• W2970405392 endingPage "18294" @default.
• W2970405392 startingPage "18285" @default.
• W2970405392 abstract "Copper is essential for life, and beyond its well-established ability to serve as a tightly bound, redox-active active site cofactor for enzyme function, emerging data suggest that cellular copper also exists in labile pools, defined as loosely bound to low-molecular-weight ligands, which can regulate diverse transition metal signaling processes spanning neural communication and olfaction, lipolysis, rest-activity cycles, and kinase pathways critical for oncogenic signaling. To help decipher this growing biology, we report a first-generation ratiometric fluorescence resonance energy transfer (FRET) copper probe, FCP-1, for activity-based sensing of labile Cu(I) pools in live cells. FCP-1 links fluorescein and rhodamine dyes through a Tris[(2-pyridyl)methyl]amine bridge. Bioinspired Cu(I)-induced oxidative cleavage decreases FRET between fluorescein donor and rhodamine acceptor. FCP-1 responds to Cu(I) with high metal selectivity and oxidation-state specificity and facilitates ratiometric measurements that minimize potential interferences arising from variations in sample thickness, dye concentration, and light intensity. FCP-1 enables imaging of dynamic changes in labile Cu(I) pools in live cells in response to copper supplementation/depletion, differential expression of the copper importer CTR1, and redox stress induced by manipulating intracellular glutathione levels and reduced/oxidized glutathione (GSH/GSSG) ratios. FCP-1 imaging reveals a labile Cu(I) deficiency induced by oncogene-driven cellular transformation that promotes fluctuations in glutathione metabolism, where lower GSH/GSSG ratios decrease labile Cu(I) availability without affecting total copper levels. By connecting copper dysregulation and glutathione stress in cancer, this work provides a valuable starting point to study broader cross-talk between metal and redox pathways in health and disease with activity-based probes." @default.
• W2970405392 created "2019-09-05" @default.
• W2970405392 creator A5006947389 @default.
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• W2970405392 creator A5088581671 @default.
• W2970405392 date "2019-08-26" @default.
• W2970405392 modified "2023-10-07" @default.
• W2970405392 title "Activity-based ratiometric FRET probe reveals oncogene-driven changes in labile copper pools induced by altered glutathione metabolism" @default.
• W2970405392 cites W1601936385 @default.
• W2970405392 cites W1870977569 @default.
• W2970405392 cites W1965550035 @default.
• W2970405392 cites W1965953865 @default.
• W2970405392 cites W1968210490 @default.
• W2970405392 cites W1969004465 @default.
• W2970405392 cites W1969524738 @default.
• W2970405392 cites W1977316589 @default.
• W2970405392 cites W1977443712 @default.
• W2970405392 cites W1977972524 @default.
• W2970405392 cites W1981532832 @default.
• W2970405392 cites W1983219543 @default.
• W2970405392 cites W1984362555 @default.
• W2970405392 cites W1984759027 @default.
• W2970405392 cites W1987530130 @default.
• W2970405392 cites W1992724001 @default.
• W2970405392 cites W1995151567 @default.
• W2970405392 cites W1995713813 @default.
• W2970405392 cites W1997790931 @default.
• W2970405392 cites W2003595893 @default.
• W2970405392 cites W2008336962 @default.
• W2970405392 cites W2011817526 @default.
• W2970405392 cites W2013976474 @default.
• W2970405392 cites W2014912105 @default.
• W2970405392 cites W2025379625 @default.
• W2970405392 cites W2026536857 @default.
• W2970405392 cites W2027081858 @default.
• W2970405392 cites W2030572315 @default.
• W2970405392 cites W2041777125 @default.
• W2970405392 cites W2051986914 @default.
• W2970405392 cites W2056625364 @default.
• W2970405392 cites W2056869450 @default.
• W2970405392 cites W2061159332 @default.
• W2970405392 cites W2063074514 @default.
• W2970405392 cites W2063157862 @default.
• W2970405392 cites W2067670037 @default.
• W2970405392 cites W2068346999 @default.
• W2970405392 cites W2071378425 @default.
• W2970405392 cites W2072383565 @default.
• W2970405392 cites W2074842308 @default.
• W2970405392 cites W2078320842 @default.
• W2970405392 cites W2079624831 @default.
• W2970405392 cites W2080493328 @default.
• W2970405392 cites W2082201973 @default.
• W2970405392 cites W2096258216 @default.
• W2970405392 cites W2097929628 @default.
• W2970405392 cites W2115660554 @default.
• W2970405392 cites W2117726740 @default.
• W2970405392 cites W2130002263 @default.
• W2970405392 cites W2131734129 @default.
• W2970405392 cites W2134390575 @default.
• W2970405392 cites W2139178065 @default.
• W2970405392 cites W2139855058 @default.
• W2970405392 cites W2141060691 @default.
• W2970405392 cites W2148904280 @default.
• W2970405392 cites W2149082710 @default.
• W2970405392 cites W2150766477 @default.
• W2970405392 cites W2156644611 @default.
• W2970405392 cites W2157657212 @default.
• W2970405392 cites W2165303545 @default.
• W2970405392 cites W2167639612 @default.
• W2970405392 cites W2228007557 @default.
• W2970405392 cites W2273624704 @default.
• W2970405392 cites W2297411548 @default.
• W2970405392 cites W2298097087 @default.
• W2970405392 cites W2317867606 @default.
• W2970405392 cites W2326903394 @default.
• W2970405392 cites W2344145825 @default.
• W2970405392 cites W2419048965 @default.
• W2970405392 cites W2496346930 @default.
• W2970405392 cites W2515467700 @default.
• W2970405392 cites W2557950738 @default.
• W2970405392 cites W2562236306 @default.
• W2970405392 cites W2603959977 @default.
• W2970405392 cites W2763277172 @default.
• W2970405392 cites W2765789198 @default.
• W2970405392 cites W2789045713 @default.
• W2970405392 cites W2805791169 @default.
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• W2970405392 doi "https://doi.org/10.1073/pnas.1904610116" @default.
• W2970405392 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6744846" @default.
• W2970405392 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31451653" @default.
• W2970405392 hasPublicationYear "2019" @default.
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