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• W2970469759 abstract "The capsular polysaccharide (CPS) structure of Campylobacter jejuni contributes to its robust fitness. Many strains contain heptose moieties in their CPS units. The precursor heptose is GDP-d-glycero-α-d-manno-heptose; modifications to the stereochemistry at C3–C6 as well as additions of methyl and phosphoramidate groups lend to the hypervariability of the C. jejuni CPS structures. Synthesis of GDP-d-glycero-α-d-manno-heptose has been described previously, but using enzymes from Aneurinibacillus thermoaerophilus DSM 10155. Here we describe the complete synthesis of GDP-d-glycero-α-d-manno-heptose using enzymes from C. jejuni NTCC 11168: Cj1152 and Cj1423–Cj1425. Our results yield kinetic parameters for these enzymes and outline a successful strategy for milligram–gram scale synthesis of GDP-d-glycero-α-d-manno-heptose. This achievement is critical for the characterization of other carbohydrate tailoring enzymes, which are expected to utilize GDP-d-glycero-α-d-manno-heptose for the biosynthesis of more complex carbohydrates in the CPS of C. jejuni." @default.
• W2970469759 created "2019-09-05" @default.
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• W2970469759 date "2019-08-26" @default.
• W2970469759 modified "2023-09-26" @default.
• W2970469759 title "Biosynthesis of GDP-<scp>d</scp>-<i>glycero</i>-α-<scp>d</scp>-<i>manno</i>-heptose for the Capsular Polysaccharide of <i>Campylobacter jejuni</i>" @default.
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• W2970469759 doi "https://doi.org/10.1021/acs.biochem.9b00548" @default.
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