FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2970549579> ?p ?o ?g. }

• W2970549579 endingPage "1967" @default.
• W2970549579 startingPage "1958" @default.
• W2970549579 abstract "The engineered de novo vanillin biosynthesis pathway constructed in Escherichia coli is industrially relevant but limited by the reaction catalyzed by catechol O-methyltransferase, which is intended to catalyze the conversion of protocatechuate to vanillate. To identify alternative O-methyltransferases, we constructed a vanillate sensor based on the Caulobacter crescentus VanR-VanO system. Using an E. coli promoter library, we achieved greater than 14-fold dynamic range in our best rationally constructed sensor. We found that this construct and an evolved variant demonstrate remarkable substrate selectivity, exhibiting no detectable response to the regioisomer byproduct isovanillate and minimal response to structurally similar pathway intermediates. We then harnessed the evolved biosensor to conduct rapid bioprospecting of natural catechol O-methyltransferases and identified three previously uncharacterized but active O-methyltransferases. Collectively, these efforts enrich our knowledge of how biosensing can aid metabolic engineering and constitute the foundation for future improvements in vanillin pathway productivity." @default.
• W2970549579 created "2019-09-05" @default.
• W2970549579 creator A5005731990 @default.
• W2970549579 creator A5061341492 @default.
• W2970549579 date "2019-08-28" @default.
• W2970549579 modified "2023-09-30" @default.
• W2970549579 title "Development of a Vanillate Biosensor for the Vanillin Biosynthesis Pathway in <i>E. coli</i>" @default.
• W2970549579 cites W1540714253 @default.
• W2970549579 cites W1971439989 @default.
• W2970549579 cites W1977813101 @default.
• W2970549579 cites W1993183179 @default.
• W2970549579 cites W2005166450 @default.
• W2970549579 cites W2006591980 @default.
• W2970549579 cites W2007757819 @default.
• W2970549579 cites W2013378149 @default.
• W2970549579 cites W2014133014 @default.
• W2970549579 cites W2018457221 @default.
• W2970549579 cites W2051468192 @default.
• W2970549579 cites W2057893359 @default.
• W2970549579 cites W2066529216 @default.
• W2970549579 cites W2073265884 @default.
• W2970549579 cites W2076769977 @default.
• W2970549579 cites W2081799587 @default.
• W2970549579 cites W2110573775 @default.
• W2970549579 cites W2118464978 @default.
• W2970549579 cites W2124180487 @default.
• W2970549579 cites W2130708697 @default.
• W2970549579 cites W2131350482 @default.
• W2970549579 cites W2135893048 @default.
• W2970549579 cites W2138072637 @default.
• W2970549579 cites W2140008132 @default.
• W2970549579 cites W2141710137 @default.
• W2970549579 cites W2145186348 @default.
• W2970549579 cites W2147433402 @default.
• W2970549579 cites W2148641266 @default.
• W2970549579 cites W2154313392 @default.
• W2970549579 cites W2160199722 @default.
• W2970549579 cites W2160758648 @default.
• W2970549579 cites W2161782059 @default.
• W2970549579 cites W2170039601 @default.
• W2970549579 cites W2171813585 @default.
• W2970549579 cites W2212931362 @default.
• W2970549579 cites W2327754503 @default.
• W2970549579 cites W2340833294 @default.
• W2970549579 cites W2564937330 @default.
• W2970549579 cites W2580966703 @default.
• W2970549579 cites W2584936337 @default.
• W2970549579 cites W2616983714 @default.
• W2970549579 cites W2791991616 @default.
• W2970549579 cites W2900498998 @default.
• W2970549579 cites W2949459453 @default.
• W2970549579 cites W2950963468 @default.
• W2970549579 cites W4244274462 @default.
• W2970549579 cites W796291325 @default.
• W2970549579 cites W847394227 @default.
• W2970549579 doi "https://doi.org/10.1021/acssynbio.9b00071" @default.
• W2970549579 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31461264" @default.
• W2970549579 hasPublicationYear "2019" @default.
• W2970549579 type Work @default.
• W2970549579 sameAs 2970549579 @default.
• W2970549579 citedByCount "39" @default.
• W2970549579 countsByYear W29705495792019 @default.
• W2970549579 countsByYear W29705495792020 @default.
• W2970549579 countsByYear W29705495792021 @default.
• W2970549579 countsByYear W29705495792022 @default.
• W2970549579 countsByYear W29705495792023 @default.
• W2970549579 crossrefType "journal-article" @default.
• W2970549579 hasAuthorship W2970549579A5005731990 @default.
• W2970549579 hasAuthorship W2970549579A5061341492 @default.
• W2970549579 hasBestOaLocation W29705495792 @default.
• W2970549579 hasConcept C104317684 @default.
• W2970549579 hasConcept C143065580 @default.
• W2970549579 hasConcept C160756335 @default.
• W2970549579 hasConcept C16318435 @default.
• W2970549579 hasConcept C181199279 @default.
• W2970549579 hasConcept C185592680 @default.
• W2970549579 hasConcept C191908910 @default.
• W2970549579 hasConcept C192989942 @default.
• W2970549579 hasConcept C2777340834 @default.
• W2970549579 hasConcept C2777713698 @default.
• W2970549579 hasConcept C2778767192 @default.
• W2970549579 hasConcept C2993175405 @default.
• W2970549579 hasConcept C33288867 @default.
• W2970549579 hasConcept C501734568 @default.
• W2970549579 hasConcept C547475151 @default.
• W2970549579 hasConcept C553450214 @default.
• W2970549579 hasConcept C55493867 @default.
• W2970549579 hasConcept C6350086 @default.
• W2970549579 hasConcept C70721500 @default.
• W2970549579 hasConcept C86803240 @default.
• W2970549579 hasConcept C91965660 @default.
• W2970549579 hasConcept C9418097 @default.
• W2970549579 hasConceptScore W2970549579C104317684 @default.
• W2970549579 hasConceptScore W2970549579C143065580 @default.
• W2970549579 hasConceptScore W2970549579C160756335 @default.
• W2970549579 hasConceptScore W2970549579C16318435 @default.
• W2970549579 hasConceptScore W2970549579C181199279 @default.
• W2970549579 hasConceptScore W2970549579C185592680 @default.

• next