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• W2970580966 abstract "Nickel-chelating lipids offer a convenient platform for reversible immobilization of histidine-tagged proteins to liposome surfaces. This interaction recently found utility as a model system for studying membrane remodeling triggered by protein crowding. Despite its wide array of utility, the molecular details of transient protein association to the lipid surfaces decorated with such chelator lipids remains poorly understood. In this study, we explore the kinetics of protein-liposome association across a wide concentration range using stopped-flow fluorescence. The fluorescence of histidine-tagged protein containing an intrinsic fluorophore (superfolder green fluorescent protein, SfGFP) was quenched upon binding to Ni-NTA-modified liposomes containing the quencher Dabsyl-PE lipids. Stopped-flow fluorescence reveals a complex, multiexponential binding behavior with a fast (kobs ∼ 10-20 s-1) phase and slower (kobs < 4 s-1) phase. Interestingly, the observed rates for the slower phase increase initially under low concentrations but start decreasing once a critical concentration is reached. Despite differences in the binding time scales, we observe that the trend of decreasing rates is reproducible irrespective of the chelator lipid doping level, protein surface charge, or lipid composition. Consideration of the protein footprint and membrane surface area occupancy leads us to conclude that the multiphasic binding behavior is reflective of protein binding via two distinct binding conformations. We propose that preliminary steps in protein association involve binding of a sterically occlusive side-on conformation followed by reorganization that leads to an end-on conformation with increased packing density. These results are important for the improvement of histidine-tag-based immobilization strategies and offer mechanistic insight into intermediates preceding membrane bending driven by protein crowding." @default.
• W2970580966 created "2019-09-05" @default.
• W2970580966 creator A5065643040 @default.
• W2970580966 creator A5082779363 @default.
• W2970580966 date "2019-08-29" @default.
• W2970580966 modified "2023-09-24" @default.
• W2970580966 title "Kinetics of Histidine-Tagged Protein Association to Nickel-Decorated Liposome Surfaces" @default.
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• W2970580966 doi "https://doi.org/10.1021/acs.langmuir.9b01700" @default.
• W2970580966 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6759406" @default.
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• W2970580966 hasPublicationYear "2019" @default.
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