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- W2970678199 abstract "Abstract The conduit network is a hallmark of lymph node microanatomy, but lack of suitable imaging technology has prevented comprehensive investigation of its topology. We employed an extended-volume imaging system to capture the conduit network of an entire murine lymph node (≈280,000 segments). The extensive 3D images provide a comprehensive overview of the regions supplied by conduits including perivascular sleeves, and distinctive “follicular reservoirs” within B cell follicles, surrounding follicular dendritic cells. A 3D topology map of conduits within the T cell zone showed homogeneous branching, but conduit density was significantly higher in the superficial T cell zone compared to the deep zone, where distances between segments are sufficient for T cells to lose contact with fibroblastic reticular cells. This topological mapping of the conduit anatomy can now aid modeling of its roles in lymph node function, as we demonstrate by simulating T cell motility in the different T cell zones." @default.
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- W2970678199 date "2019-08-30" @default.
- W2970678199 modified "2023-09-27" @default.
- W2970678199 title "High-resolution 3D imaging and topological mapping of the lymph node conduit system" @default.
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- W2970678199 doi "https://doi.org/10.1101/752972" @default.
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