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• W297086659 abstract "Sugarcane yellow leaf syndrome (YLS) is a relatively new disease of sugarcane. YLS was first noted in Hawaii in 1988 and has subsequently been reported from a wide range of sugarcane growing countries throughout the world. A virus, now commonly referred to as Sugarcane Yellow Leaf Virus (SCYLV) is associated with this syndrome. Analysis of the complete SCYLV genome has shown it to be a new member of the family Luteoviridae. It is possible the highly variable nature of the degree of YLS symptomology observed in SCYLV-infected sugarcane plants worldwide may be due to genetic variation of the virus. Fingerprinting the viral sequence from various SCYLV positive cultivars has revealed there is some degree of diversity in SCYLV sequences both .from and within different geographic locations. Introduction The variable nature of YLS symptoms observed A leaf-yellowing symptom of sugarcane in SCYLV-infected sugarcane plants at a wide range (Saccharum L. interspecific hybrids) was first noted of locales may be a consequence of genomic in Hawaii in 1988 (Schenck, 1990). Four years later variation of the virus. TO date, there has been no the disease, yellow leaf syndrome (YLS), was wideidentification of strains of this virus. The aim of this spread in Brazil and it has since been reported from study was to determine the extent of genomic the continental USA (Comstock et al., 1994), Austvariation in SCYLV isolates from various countries. ralia (Smith et al., 1995), Mauritius (Anon., 1995) and South Africa (Bailey et al., 1996). The adaxial Materials and methods surface of the leaf midrib of plants showing YLS becomes yellow and sometimes reddened or red Testing for SCYLV flecked (Comstock et al., 1994; Vega et al., 1997). Varieties from different countries were tested by a There is also an increase in brix (total soluble solids) RT-PCR assay using the SCYLV specific primers in the leaves (Comstock et al., 1994). Small icosaheYLS 111IYLS 462 (Irey et al., 1997). Of the 114 dral particles have been found in symptomatic plants samples tested to date, 74 were positive. A selection (Anon, 1995), and the aphid, Melanaphis sacchari, of positive plants were used in subsequent has been shown to transmit a virus, by now comKT-PCRIRFLP analyses. monly called Sugarcane yellow leaf virus (SCYLV), from infected to healthy sugarcane (Scagliusi and Production and purification of amplicons used Lockhart, 1997). Plants infected in this way develin fingerprinting studies oped typical symptoms and particles were isolated from them (Scagliusi and Lockhart, 1999). A weak Amplicons designated as PRO-REP ( W t e a s e to serological reaction, and the physico-chemical propE l i c a s e ) , REP-UTR ( m i c a s e to UnTranslated erties of the particles suggested that SCYLV is a luteRegion and beginning of protein), and CP-RT ovirid (Scagliusi and Lockhart,l997; Vega et al., (Capsid Protein to beginning of capsid Readzhrough 1997). This was confirmed (key et al., 1997) when a protein region) were produced using short stretch of sequence from the scYLV genome These areas indicated regions of the genome which was found to be most similar to sequence from exhibited the highest conservation compared to other Barley yellow dwarf luteovirus type PAV. To date, members of the Luteoviridae (Moonan et al. 2000). only two isolates of SCYLV have been extensively Amplicons designated ORF 1-2 (beginning of studied. One is an isolate found in variety CP65-357 ORF 1 through to ORF 2) and RT (capsid from Florida, which has been fully sequenced by two ReadThrough protein section) were also synthesised. independent laboratories (Moonan et al. 2000, Smith All RT-PCR products were gel purified for RFLP et al. 2000). The other is an isolate from Brazil analysis and digested with either Taql, Sau3Al (or a infecting cultivar SP71-6163 (Vega et al. 1997), for combination of the two), Hhal or HaeIII to produce which full genomic sequence is not available. fingerprint profiles." @default.
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• W297086659 date "2001-01-01" @default.
• W297086659 modified "2023-09-27" @default.
• W297086659 title "Characterising the genetic diversity of sugarcane yellow leaf virus." @default.
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