Matches in SemOpenAlex for { <https://semopenalex.org/work/W2970996943> ?p ?o ?g. }
- W2970996943 endingPage "5031" @default.
- W2970996943 startingPage "5027" @default.
- W2970996943 abstract "Abstract Carbon‐carbon bond formation is a fundamental transformation in both synthetic chemistry and biosynthesis. Enzymes catalyze such reactions with exquisite selectivity which often cannot be achieved using non‐biological methods but may suffer from an intolerance of high temperature and the presence of organic solvents limiting their applications. Here we report the thermodynamic and kinetic stability of the β ‐barrel natural Diels‐Alderase AbyU, which catalyzes formation of the spirotetronate core of the antimicrobial natural product abyssomicin C, with creation of 3 new asymmetric centers. This enzyme is shown to catalyze [4+2] cycloadditions at elevated temperature (up to 65 °C), and in the presence of organic solvents (MeOH, CH 3 CN and DMSO) and the chemical denaturant guanidinium hydrochloride, revealing that AbyU has potential widespread value as a biocatalyst." @default.
- W2970996943 created "2019-09-05" @default.
- W2970996943 creator A5011398930 @default.
- W2970996943 creator A5012519742 @default.
- W2970996943 creator A5016544097 @default.
- W2970996943 creator A5027167340 @default.
- W2970996943 creator A5028083645 @default.
- W2970996943 creator A5051336701 @default.
- W2970996943 creator A5063185712 @default.
- W2970996943 creator A5067138797 @default.
- W2970996943 creator A5074383030 @default.
- W2970996943 creator A5077744141 @default.
- W2970996943 creator A5083529762 @default.
- W2970996943 creator A5088782116 @default.
- W2970996943 date "2019-09-30" @default.
- W2970996943 modified "2023-10-09" @default.
- W2970996943 title "A Natural Diels‐Alder Biocatalyst Enables Efficient [4+2] Cycloaddition Under Harsh Reaction Conditions" @default.
- W2970996943 cites W2000547662 @default.
- W2970996943 cites W2014280607 @default.
- W2970996943 cites W2025938842 @default.
- W2970996943 cites W2034011943 @default.
- W2970996943 cites W2066381187 @default.
- W2970996943 cites W2083800655 @default.
- W2970996943 cites W2109746320 @default.
- W2970996943 cites W2110773017 @default.
- W2970996943 cites W2346461087 @default.
- W2970996943 cites W2412082922 @default.
- W2970996943 cites W2470087878 @default.
- W2970996943 cites W2549976854 @default.
- W2970996943 cites W2580767461 @default.
- W2970996943 doi "https://doi.org/10.1002/cctc.201901285" @default.
- W2970996943 hasPublicationYear "2019" @default.
- W2970996943 type Work @default.
- W2970996943 sameAs 2970996943 @default.
- W2970996943 citedByCount "5" @default.
- W2970996943 countsByYear W29709969432020 @default.
- W2970996943 countsByYear W29709969432021 @default.
- W2970996943 countsByYear W29709969432023 @default.
- W2970996943 crossrefType "journal-article" @default.
- W2970996943 hasAuthorship W2970996943A5011398930 @default.
- W2970996943 hasAuthorship W2970996943A5012519742 @default.
- W2970996943 hasAuthorship W2970996943A5016544097 @default.
- W2970996943 hasAuthorship W2970996943A5027167340 @default.
- W2970996943 hasAuthorship W2970996943A5028083645 @default.
- W2970996943 hasAuthorship W2970996943A5051336701 @default.
- W2970996943 hasAuthorship W2970996943A5063185712 @default.
- W2970996943 hasAuthorship W2970996943A5067138797 @default.
- W2970996943 hasAuthorship W2970996943A5074383030 @default.
- W2970996943 hasAuthorship W2970996943A5077744141 @default.
- W2970996943 hasAuthorship W2970996943A5083529762 @default.
- W2970996943 hasAuthorship W2970996943A5088782116 @default.
- W2970996943 hasBestOaLocation W29709969432 @default.
- W2970996943 hasConcept C114373084 @default.
- W2970996943 hasConcept C161790260 @default.
- W2970996943 hasConcept C178790620 @default.
- W2970996943 hasConcept C185592680 @default.
- W2970996943 hasConcept C186399102 @default.
- W2970996943 hasConcept C191503008 @default.
- W2970996943 hasConcept C202751555 @default.
- W2970996943 hasConcept C21951064 @default.
- W2970996943 hasConcept C2779664164 @default.
- W2970996943 hasConcept C535685238 @default.
- W2970996943 hasConcept C55493867 @default.
- W2970996943 hasConcept C65024703 @default.
- W2970996943 hasConceptScore W2970996943C114373084 @default.
- W2970996943 hasConceptScore W2970996943C161790260 @default.
- W2970996943 hasConceptScore W2970996943C178790620 @default.
- W2970996943 hasConceptScore W2970996943C185592680 @default.
- W2970996943 hasConceptScore W2970996943C186399102 @default.
- W2970996943 hasConceptScore W2970996943C191503008 @default.
- W2970996943 hasConceptScore W2970996943C202751555 @default.
- W2970996943 hasConceptScore W2970996943C21951064 @default.
- W2970996943 hasConceptScore W2970996943C2779664164 @default.
- W2970996943 hasConceptScore W2970996943C535685238 @default.
- W2970996943 hasConceptScore W2970996943C55493867 @default.
- W2970996943 hasConceptScore W2970996943C65024703 @default.
- W2970996943 hasFunder F4320334629 @default.
- W2970996943 hasIssue "20" @default.
- W2970996943 hasLocation W29709969431 @default.
- W2970996943 hasLocation W29709969432 @default.
- W2970996943 hasLocation W29709969433 @default.
- W2970996943 hasLocation W29709969434 @default.
- W2970996943 hasOpenAccess W2970996943 @default.
- W2970996943 hasPrimaryLocation W29709969431 @default.
- W2970996943 hasRelatedWork W1989787195 @default.
- W2970996943 hasRelatedWork W2023085082 @default.
- W2970996943 hasRelatedWork W2111310989 @default.
- W2970996943 hasRelatedWork W2147537758 @default.
- W2970996943 hasRelatedWork W2317043810 @default.
- W2970996943 hasRelatedWork W2334674672 @default.
- W2970996943 hasRelatedWork W2516558859 @default.
- W2970996943 hasRelatedWork W2949185999 @default.
- W2970996943 hasRelatedWork W2951609808 @default.
- W2970996943 hasRelatedWork W3148072343 @default.
- W2970996943 hasVolume "11" @default.
- W2970996943 isParatext "false" @default.
- W2970996943 isRetracted "false" @default.
- W2970996943 magId "2970996943" @default.