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- W2971022014 abstract "Extracellular vesicles (EVs) contribute to a variety of signaling processes and the overall physiological and pathological states of stem cells and tissues. Human induced pluripotent stem cells (hiPSCs) have unique characteristics that can mimic embryonic tissue development. There is growing interest in the use of EVs derived from hiPSCs as therapeutics, biomarkers, and drug delivery vehicles. However, little is known about the characteristics of EVs secreted by hiPSCs and paracrine signaling during tissue morphogenesis and lineage specification. Methods: In this study, the physical and biological properties of EVs isolated from hiPSC-derived neural progenitors (ectoderm), hiPSC-derived cardiac cells (mesoderm), and the undifferentiated hiPSCs (healthy iPSK3 and Alzheimer’s-associated SY-UBH lines) were analyzed. Results: Nanoparticle tracking analysis and electron microscopy results indicate that hiPSC-derived EVs have an average size of 100–250 nm. Immunoblot analyses confirmed the enrichment of exosomal markers Alix, CD63, TSG101, and Hsc70 in the purified EV preparations. MicroRNAs including miR-133, miR-155, miR-221, and miR-34a were differently expressed in the EVs isolated from distinct hiPSC lineages. Treatment of cortical spheroids with hiPSC-EVs in vitro resulted in enhanced cell proliferation (indicated by BrdU+ cells) and axonal growth (indicated by β-tubulin III staining). Furthermore, hiPSC-derived EVs exhibited neural protective abilities in Aβ42 oligomer-treated cultures, enhancing cell viability and reducing oxidative stress. Our results demonstrate that the paracrine signaling provided by tissue context-dependent EVs derived from hiPSCs elicit distinct responses to impact the physiological state of cortical spheroids. Overall, this study advances our understanding of cell‒cell communication in the stem cell microenvironment and provides possible therapeutic options for treating neural degeneration." @default.
- W2971022014 created "2019-09-05" @default.
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- W2971022014 date "2019-08-28" @default.
- W2971022014 modified "2023-10-15" @default.
- W2971022014 title "Differential Effects of Extracellular Vesicles of Lineage-Specific Human Pluripotent Stem Cells on the Cellular Behaviors of Isogenic Cortical Spheroids" @default.
- W2971022014 cites W1126171815 @default.
- W2971022014 cites W1553983967 @default.
- W2971022014 cites W1632791076 @default.
- W2971022014 cites W1646577443 @default.
- W2971022014 cites W1700582724 @default.
- W2971022014 cites W1808455042 @default.
- W2971022014 cites W1843099843 @default.
- W2971022014 cites W1966758310 @default.
- W2971022014 cites W1975320047 @default.
- W2971022014 cites W1989339219 @default.
- W2971022014 cites W1992048306 @default.
- W2971022014 cites W2011537287 @default.
- W2971022014 cites W2018286352 @default.
- W2971022014 cites W2020755558 @default.
- W2971022014 cites W2041014303 @default.
- W2971022014 cites W2052147188 @default.
- W2971022014 cites W2057356848 @default.
- W2971022014 cites W2058180357 @default.
- W2971022014 cites W2065561114 @default.
- W2971022014 cites W2067363138 @default.
- W2971022014 cites W2076289829 @default.
- W2971022014 cites W2088714934 @default.
- W2971022014 cites W2097163538 @default.
- W2971022014 cites W2098259366 @default.
- W2971022014 cites W2126231259 @default.
- W2971022014 cites W2138574694 @default.
- W2971022014 cites W2159278398 @default.
- W2971022014 cites W2166305181 @default.
- W2971022014 cites W2170449834 @default.
- W2971022014 cites W2187996036 @default.
- W2971022014 cites W2266238367 @default.
- W2971022014 cites W2325783815 @default.
- W2971022014 cites W2338671470 @default.
- W2971022014 cites W2338920735 @default.
- W2971022014 cites W2341041396 @default.
- W2971022014 cites W2408788996 @default.
- W2971022014 cites W2463753610 @default.
- W2971022014 cites W2466551530 @default.
- W2971022014 cites W2506498379 @default.
- W2971022014 cites W2563244430 @default.
- W2971022014 cites W2571952474 @default.
- W2971022014 cites W2580975427 @default.
- W2971022014 cites W2587737921 @default.
- W2971022014 cites W2591763108 @default.
- W2971022014 cites W2596705496 @default.
- W2971022014 cites W2609473806 @default.
- W2971022014 cites W2612533830 @default.
- W2971022014 cites W2620679532 @default.
- W2971022014 cites W2625200959 @default.
- W2971022014 cites W2735206626 @default.
- W2971022014 cites W2741286864 @default.
- W2971022014 cites W2742059672 @default.
- W2971022014 cites W2748470793 @default.
- W2971022014 cites W2752231537 @default.
- W2971022014 cites W2762018708 @default.
- W2971022014 cites W2762841905 @default.
- W2971022014 cites W2765473209 @default.
- W2971022014 cites W2767421147 @default.
- W2971022014 cites W2772938962 @default.
- W2971022014 cites W2777343869 @default.
- W2971022014 cites W2781480706 @default.
- W2971022014 cites W2781817102 @default.
- W2971022014 cites W2783040166 @default.
- W2971022014 cites W2784363986 @default.
- W2971022014 cites W2784614257 @default.
- W2971022014 cites W2786514708 @default.
- W2971022014 cites W2792994524 @default.
- W2971022014 cites W2793032642 @default.
- W2971022014 cites W2796652469 @default.
- W2971022014 cites W2797032954 @default.
- W2971022014 cites W2801771391 @default.
- W2971022014 cites W2802715752 @default.
- W2971022014 cites W2805396342 @default.
- W2971022014 cites W2805401782 @default.
- W2971022014 cites W2807094840 @default.
- W2971022014 cites W2811028332 @default.
- W2971022014 cites W2811209460 @default.
- W2971022014 cites W2887249435 @default.
- W2971022014 cites W2891937142 @default.
- W2971022014 cites W2896130761 @default.
- W2971022014 cites W2902098438 @default.
- W2971022014 cites W2903613075 @default.
- W2971022014 cites W2905324087 @default.