FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2971058817> ?p ?o ?g. }

• W2971058817 endingPage "505" @default.
• W2971058817 startingPage "499" @default.
• W2971058817 abstract "Mumps is a common type of respiratory infectious disease caused by mumps virus (MuV), and can be effectively prevented by vaccination. In this study, a reverse genetic system of MuV that can facilitate the rational design of safer, more efficient mumps vaccine candidates is established.MuV-S79 cDNA clone was assembled into a full-length plasmid by means of the GeneArt™ High-Order Genetic Assembly System, and was rescued via reverse genetic technology. RT-PCR, sequencing, and immunofluorescence assays were used for rMuV-S79 authentication. Viral replication kinetics and in vivo experimental models were used to evaluate the replication, safety, and immunogenicity of rMuV-S79.A full-length cDNA clone of MuV-S79 in the assembly process was generated by a novel plasmid assemble strategy, and a robust reverse genetic system of MuV-S79 was successfully established. The established rMuV-S79 strain could reach a high virus titer in vitro. The average viral titer of rMuV-S79 in the lung tissues was 2.68 ± 0.14 log10PFU/g lung tissue, and rMuV-S79 group did not induce inflammation in the lung tissues in cotton rats. Neutralizing antibody titers induced by rMuV-S79 were high, long-lasting and could provide complete protection against MuV wild strain challenge.We have established a robust reverse genetic system of MuV-S79 which can facilitate the optimization of mumps vaccines. rMuV-S79 rescued could reach a high virus titer and the safety was proven in vivo. It could also provide complete protection against MuV wild strain challenge." @default.
• W2971058817 created "2019-09-05" @default.
• W2971058817 creator A5006443452 @default.
• W2971058817 creator A5013612077 @default.
• W2971058817 creator A5028869390 @default.
• W2971058817 creator A5033981529 @default.
• W2971058817 creator A5052504970 @default.
• W2971058817 creator A5073790560 @default.
• W2971058817 creator A5082703869 @default.
• W2971058817 creator A5087101695 @default.
• W2971058817 creator A5091507303 @default.
• W2971058817 date "2019-08-28" @default.
• W2971058817 modified "2023-09-27" @default.
• W2971058817 title "Establishment of an efficient reverse genetic system of Mumps virus S79 from cloned DNA" @default.
• W2971058817 cites W1810300540 @default.
• W2971058817 cites W1833141022 @default.
• W2971058817 cites W1863653662 @default.
• W2971058817 cites W1955376530 @default.
• W2971058817 cites W1973485958 @default.
• W2971058817 cites W1979255492 @default.
• W2971058817 cites W2043152280 @default.
• W2971058817 cites W2044624104 @default.
• W2971058817 cites W2045216516 @default.
• W2971058817 cites W2059288494 @default.
• W2971058817 cites W2064727744 @default.
• W2971058817 cites W2084461655 @default.
• W2971058817 cites W2109387251 @default.
• W2971058817 cites W2114218202 @default.
• W2971058817 cites W2133434608 @default.
• W2971058817 cites W2135025635 @default.
• W2971058817 cites W2142994110 @default.
• W2971058817 cites W2151432952 @default.
• W2971058817 cites W2154311334 @default.
• W2971058817 cites W2159213937 @default.
• W2971058817 cites W2159779332 @default.
• W2971058817 cites W2165853224 @default.
• W2971058817 cites W2195800150 @default.
• W2971058817 cites W2422270755 @default.
• W2971058817 cites W2571892062 @default.
• W2971058817 cites W2792432251 @default.
• W2971058817 cites W4250386923 @default.
• W2971058817 cites W76787895 @default.
• W2971058817 doi "https://doi.org/10.1007/s12519-019-00286-8" @default.
• W2971058817 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6785654" @default.
• W2971058817 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31456156" @default.
• W2971058817 hasPublicationYear "2019" @default.
• W2971058817 type Work @default.
• W2971058817 sameAs 2971058817 @default.
• W2971058817 citedByCount "5" @default.
• W2971058817 countsByYear W29710588172020 @default.
• W2971058817 countsByYear W29710588172021 @default.
• W2971058817 countsByYear W29710588172023 @default.
• W2971058817 crossrefType "journal-article" @default.
• W2971058817 hasAuthorship W2971058817A5006443452 @default.
• W2971058817 hasAuthorship W2971058817A5013612077 @default.
• W2971058817 hasAuthorship W2971058817A5028869390 @default.
• W2971058817 hasAuthorship W2971058817A5033981529 @default.
• W2971058817 hasAuthorship W2971058817A5052504970 @default.
• W2971058817 hasAuthorship W2971058817A5073790560 @default.
• W2971058817 hasAuthorship W2971058817A5082703869 @default.
• W2971058817 hasAuthorship W2971058817A5087101695 @default.
• W2971058817 hasAuthorship W2971058817A5091507303 @default.
• W2971058817 hasBestOaLocation W29710588171 @default.
• W2971058817 hasConcept C104317684 @default.
• W2971058817 hasConcept C143065580 @default.
• W2971058817 hasConcept C159047783 @default.
• W2971058817 hasConcept C159654299 @default.
• W2971058817 hasConcept C167508917 @default.
• W2971058817 hasConcept C203014093 @default.
• W2971058817 hasConcept C22070199 @default.
• W2971058817 hasConcept C22744801 @default.
• W2971058817 hasConcept C2522874641 @default.
• W2971058817 hasConcept C25365240 @default.
• W2971058817 hasConcept C2776438120 @default.
• W2971058817 hasConcept C2776672420 @default.
• W2971058817 hasConcept C2778538096 @default.
• W2971058817 hasConcept C2778814567 @default.
• W2971058817 hasConcept C2780868878 @default.
• W2971058817 hasConcept C32611913 @default.
• W2971058817 hasConcept C54355233 @default.
• W2971058817 hasConcept C552990157 @default.
• W2971058817 hasConcept C86803240 @default.
• W2971058817 hasConceptScore W2971058817C104317684 @default.
• W2971058817 hasConceptScore W2971058817C143065580 @default.
• W2971058817 hasConceptScore W2971058817C159047783 @default.
• W2971058817 hasConceptScore W2971058817C159654299 @default.
• W2971058817 hasConceptScore W2971058817C167508917 @default.
• W2971058817 hasConceptScore W2971058817C203014093 @default.
• W2971058817 hasConceptScore W2971058817C22070199 @default.
• W2971058817 hasConceptScore W2971058817C22744801 @default.
• W2971058817 hasConceptScore W2971058817C2522874641 @default.
• W2971058817 hasConceptScore W2971058817C25365240 @default.
• W2971058817 hasConceptScore W2971058817C2776438120 @default.
• W2971058817 hasConceptScore W2971058817C2776672420 @default.
• W2971058817 hasConceptScore W2971058817C2778538096 @default.
• W2971058817 hasConceptScore W2971058817C2778814567 @default.
• W2971058817 hasConceptScore W2971058817C2780868878 @default.
• W2971058817 hasConceptScore W2971058817C32611913 @default.

• next