Matches in SemOpenAlex for { <https://semopenalex.org/work/W2971213397> ?p ?o ?g. }
Showing items 1 to 50 of
50
with 100 items per page.
- W2971213397 abstract "Abstract Background In 1984, Susumu Ohno hypothesized that the nylon-degrading enzyme NylB arose de novo via a frameshift mutation within a hypothetical precursor protein (PR.C). However, the frameshift hypothesis was never actually tested, and there was no actual supporting biological evidence supporting the hypothesis. For decades, the frameshift hypothesis has been uncritically accepted as the correct explanation for the origin of NylB. In this paper we have surveyed the literature relevant to the frameshift hypothesis as well as the various alternative models that have been published regarding the origin of NylB. We have employed bioinformatic methods and leveraged databases not available when the frameshift hypothesis was first put forward.Results We searched multiple protein databases to determine the distribution of NylB and any possible homologs. We then determined the distribution of other known nylonases and their possible homologs. We also determined the distribution of Ohno’s hypothetical PR.C. protein and any possible homologs. Lastly, we determined what protein families the various nylonases belong to. We found that the NylB protein is widely occurring, has thousands of homologs, and is found in diverse organisms and diverse habitats. It is not a new or unique protein. Likewise, we found that the other known nylonases are also widely occurring, have thousands of homologs, and are found in diverse organisms and diverse habitats. However, the hypothetical PR.C protein does not show up in any of the same databases, and there is no evidence of any homologs. Conserved domain searches showed that NylB is a member of the beta lactamase protein family – a highly conserved family of enzymes. Likewise, the other known nylonases belong to well-characterized enzyme families.Conclusions Our results very effectively falsify the NylB frameshift hypothesis, while they strongly support an alternative hypothesis by Yomo. Like NylB, none of the other nylonases that we examined were substantively new or unique. All had thousands of homologs, and they were found in diverse organisms and diverse habitats. Our findings not only falsify the NylB frameshift hypothesis, they also falsify the long-held assumption that all nylonases evolved after the invention of nylon in 1935." @default.
- W2971213397 created "2019-09-05" @default.
- W2971213397 creator A5015113906 @default.
- W2971213397 creator A5042615650 @default.
- W2971213397 date "2020-01-09" @default.
- W2971213397 modified "2023-09-25" @default.
- W2971213397 title "Testing the Hypothesis that the Nylonase NylB Protein Arose de novo via a Frameshift Mutation" @default.
- W2971213397 doi "https://doi.org/10.21203/rs.2.20423/v1" @default.
- W2971213397 hasPublicationYear "2020" @default.
- W2971213397 type Work @default.
- W2971213397 sameAs 2971213397 @default.
- W2971213397 citedByCount "1" @default.
- W2971213397 countsByYear W29712133972020 @default.
- W2971213397 crossrefType "posted-content" @default.
- W2971213397 hasAuthorship W2971213397A5015113906 @default.
- W2971213397 hasAuthorship W2971213397A5042615650 @default.
- W2971213397 hasBestOaLocation W29712133971 @default.
- W2971213397 hasConcept C104317684 @default.
- W2971213397 hasConcept C29906990 @default.
- W2971213397 hasConcept C501734568 @default.
- W2971213397 hasConcept C54355233 @default.
- W2971213397 hasConcept C64894306 @default.
- W2971213397 hasConcept C70721500 @default.
- W2971213397 hasConcept C78458016 @default.
- W2971213397 hasConcept C86803240 @default.
- W2971213397 hasConceptScore W2971213397C104317684 @default.
- W2971213397 hasConceptScore W2971213397C29906990 @default.
- W2971213397 hasConceptScore W2971213397C501734568 @default.
- W2971213397 hasConceptScore W2971213397C54355233 @default.
- W2971213397 hasConceptScore W2971213397C64894306 @default.
- W2971213397 hasConceptScore W2971213397C70721500 @default.
- W2971213397 hasConceptScore W2971213397C78458016 @default.
- W2971213397 hasConceptScore W2971213397C86803240 @default.
- W2971213397 hasLocation W29712133971 @default.
- W2971213397 hasOpenAccess W2971213397 @default.
- W2971213397 hasPrimaryLocation W29712133971 @default.
- W2971213397 hasRelatedWork W10419168 @default.
- W2971213397 hasRelatedWork W10844698 @default.
- W2971213397 hasRelatedWork W11242803 @default.
- W2971213397 hasRelatedWork W161059 @default.
- W2971213397 hasRelatedWork W2594801 @default.
- W2971213397 hasRelatedWork W2763321 @default.
- W2971213397 hasRelatedWork W3009669 @default.
- W2971213397 hasRelatedWork W3811461 @default.
- W2971213397 hasRelatedWork W8930798 @default.
- W2971213397 hasRelatedWork W1678534 @default.
- W2971213397 isParatext "false" @default.
- W2971213397 isRetracted "false" @default.
- W2971213397 magId "2971213397" @default.
- W2971213397 workType "article" @default.