FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2971223908> ?p ?o ?g. }

• W2971223908 abstract "Abstract The opportunistic pathogens Burkholderia cepacia and Burkholderia contaminans , both genomovars of the Burkholderia cepacia complex (BCC), are frequently cultured from the potable water system (PWS) of the International Space Station (ISS). Here, we sequenced the genomes and conducted phenotypic assays to characterize these Burkholderia isolates. All recovered isolates of the two species fall within monophyletic clades based on phylogenomic trees of conserved single-copy core genes. Within species, the ISS PWS strains all demonstrate greater than 99% average nucleotide identity (ANI), suggesting that they are of a highly similar genomic lineage and both individually may have stemmed from the two founding clonal strains before diverging into two unique sub strain populations. No evidence for horizontal gene transfer between the populations was observed. Differences between the recovered isolates can be observed at the pangenomic level, particularly within putative plasmids identified within the B. cepacia group. Phenotypically, the ISS-derived B. cepacia isolates generally exhibit a trend of lower rates and shorter duration of macrophage intracellularization compared to the selected terrestrial reference strain (though not significantly), and significantly lower rates of cellular lysis in 7 of the 19 isolates. ISS-derived B. contaminans isolates displayed no difference in rates of macrophage intracellularization compared to the selected reference, though generally increased rates lysis, with 2 of the 5 significantly increased at 12-hours post inoculation. We additionally find that ISS-isolated B. contaminans display hemolytic activity at 37°C not demonstrated by the terrestrial control, and greater antifungal capacity in the more recently collected isolates. Thankfully, the ISS-derived isolates generally exhibit 1-4 times greater sensitivity to common antibiotics used in their clinical treatments. Thus, despite their infection potential, therapeutic treatment should still have efficacy. Author Summary The International Space Station (ISS) is a unique built environment due to its isolation and recycling of air and water. Both microbes and astronauts inhabit the ISS, and the potential pathogenicity of the former is of great concern for the safety of the latter. The potable water dispenser (PWD) of the potable water system (PWS) on board the ISS was assembled in a cleanroom facility and then primed on Earth using an extensive process to ensure no gas bubbles existed within the lines that could lock the apparatus upon installation in orbit. The primed system sat dormant for 6 months before installation on the ISS. Microbial surveillance was conducted on the system after installation and the bacterial load was 85 CFU/mL, which exceeded the 50 CFU/mL limits set for ISS potable water. Over a microbial surveillance spanning 4.5 years, numerous strains of the potential pathogen Burkholderia have been isolated from the PWD. Here we sequenced and analyzed the genomes of these strains while also characterizing their potential pathogenicity. The genome analysis indicates it is likely that there were only two strains that were introduced on Earth that have subsequently undergone minimal diverging evolution. These strains retain pathogenicity, but remain susceptible to antibiotics, providing a potential therapeutic intervention in the event of infection." @default.
• W2971223908 created "2019-09-05" @default.
• W2971223908 creator A5006349615 @default.
• W2971223908 creator A5023016225 @default.
• W2971223908 creator A5050578982 @default.
• W2971223908 creator A5069667659 @default.
• W2971223908 date "2019-08-30" @default.
• W2971223908 modified "2023-09-25" @default.
• W2971223908 title "Genomic and phenotypic characterization ofBurkholderiaisolates from the potable water system of the International Space Station" @default.
• W2971223908 cites W1483833825 @default.
• W2971223908 cites W1933238348 @default.
• W2971223908 cites W1958058086 @default.
• W2971223908 cites W1964850327 @default.
• W2971223908 cites W1965591002 @default.
• W2971223908 cites W1968546690 @default.
• W2971223908 cites W1972785894 @default.
• W2971223908 cites W2003935269 @default.
• W2971223908 cites W2031611770 @default.
• W2971223908 cites W2032773353 @default.
• W2971223908 cites W2059462675 @default.
• W2971223908 cites W2061994750 @default.
• W2971223908 cites W2069586001 @default.
• W2971223908 cites W2069712815 @default.
• W2971223908 cites W2077403380 @default.
• W2971223908 cites W2093188199 @default.
• W2971223908 cites W2113174435 @default.
• W2971223908 cites W2118442768 @default.
• W2971223908 cites W2120902911 @default.
• W2971223908 cites W2129197306 @default.
• W2971223908 cites W2131271579 @default.
• W2971223908 cites W2135639274 @default.
• W2971223908 cites W2136632285 @default.
• W2971223908 cites W2147722400 @default.
• W2971223908 cites W2148703390 @default.
• W2971223908 cites W2334508509 @default.
• W2971223908 cites W2511671681 @default.
• W2971223908 cites W2581831223 @default.
• W2971223908 cites W2588816418 @default.
• W2971223908 cites W2806893779 @default.
• W2971223908 cites W2949420657 @default.
• W2971223908 cites W2951254987 @default.
• W2971223908 cites W2951563276 @default.
• W2971223908 cites W2964884626 @default.
• W2971223908 cites W97059505 @default.
• W2971223908 doi "https://doi.org/10.1101/753087" @default.
• W2971223908 hasPublicationYear "2019" @default.
• W2971223908 type Work @default.
• W2971223908 sameAs 2971223908 @default.
• W2971223908 citedByCount "0" @default.
• W2971223908 crossrefType "posted-content" @default.
• W2971223908 hasAuthorship W2971223908A5006349615 @default.
• W2971223908 hasAuthorship W2971223908A5023016225 @default.
• W2971223908 hasAuthorship W2971223908A5050578982 @default.
• W2971223908 hasAuthorship W2971223908A5069667659 @default.
• W2971223908 hasBestOaLocation W29712239081 @default.
• W2971223908 hasConcept C104317684 @default.
• W2971223908 hasConcept C105702510 @default.
• W2971223908 hasConcept C127716648 @default.
• W2971223908 hasConcept C2776817793 @default.
• W2971223908 hasConcept C2777756538 @default.
• W2971223908 hasConcept C2778022156 @default.
• W2971223908 hasConcept C2780855054 @default.
• W2971223908 hasConcept C523546767 @default.
• W2971223908 hasConcept C54355233 @default.
• W2971223908 hasConcept C86803240 @default.
• W2971223908 hasConcept C89423630 @default.
• W2971223908 hasConceptScore W2971223908C104317684 @default.
• W2971223908 hasConceptScore W2971223908C105702510 @default.
• W2971223908 hasConceptScore W2971223908C127716648 @default.
• W2971223908 hasConceptScore W2971223908C2776817793 @default.
• W2971223908 hasConceptScore W2971223908C2777756538 @default.
• W2971223908 hasConceptScore W2971223908C2778022156 @default.
• W2971223908 hasConceptScore W2971223908C2780855054 @default.
• W2971223908 hasConceptScore W2971223908C523546767 @default.
• W2971223908 hasConceptScore W2971223908C54355233 @default.
• W2971223908 hasConceptScore W2971223908C86803240 @default.
• W2971223908 hasConceptScore W2971223908C89423630 @default.
• W2971223908 hasLocation W29712239081 @default.
• W2971223908 hasLocation W29712239082 @default.
• W2971223908 hasOpenAccess W2971223908 @default.
• W2971223908 hasPrimaryLocation W29712239081 @default.
• W2971223908 hasRelatedWork W2002201903 @default.
• W2971223908 hasRelatedWork W2159894668 @default.
• W2971223908 hasRelatedWork W2598377376 @default.
• W2971223908 hasRelatedWork W2618344387 @default.
• W2971223908 hasRelatedWork W2754618462 @default.
• W2971223908 hasRelatedWork W2782840337 @default.
• W2971223908 hasRelatedWork W2786295677 @default.
• W2971223908 hasRelatedWork W2790187082 @default.
• W2971223908 hasRelatedWork W2791372647 @default.
• W2971223908 hasRelatedWork W2809888306 @default.
• W2971223908 hasRelatedWork W2867752270 @default.
• W2971223908 hasRelatedWork W2906175690 @default.
• W2971223908 hasRelatedWork W2912718854 @default.
• W2971223908 hasRelatedWork W3016479392 @default.
• W2971223908 hasRelatedWork W3082591342 @default.
• W2971223908 hasRelatedWork W3119236907 @default.
• W2971223908 hasRelatedWork W3134848036 @default.
• W2971223908 hasRelatedWork W3200044466 @default.
• W2971223908 hasRelatedWork W2782171506 @default.

• next