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• W2971325685 abstract "Abstract Background Several studies have been conducted to compare the use DBS as alternative to plasma specimens, but mainly using Whatman 903 ® cards as filter paper. The aim of this study was to evaluate Whatman FTA ® cards (FTA cards) specimens for HIV-1 viral load testing by comparing it to plasma specimens, using 2 real-Time PCR assays. Methodology A cross-sectional study was conducted between April 2017 and September 2017, in HIV-1 patients admitted at Yalgado Ouédraogo teaching hospital. Paired FTA cards and plasma specimens were collected and analyzed using Abbott RealTi m e HIV-1 assay (Abbott) and COBAS ® AmpliPrep/COBAS ® TaqMan v2.0 (Roche), following manufacturers’ protocol. Results A total of 107 patients were included. No Statistical differences (p-value > 0.05) were observed between the mean viral loads obtained from FTA cards and plasma specimens with Roche and Abbott assays. Twenty-nine samples with Roche and 15 samples with Abbott assay showed discrepant results. At viral loads of ≤1000 copies/mL, the sensitivity and specificity of FTA cards were 78.6%, and 100% with Roche, and 92.3% and 95.9% with Abbott. Strong correlation was found between FTA cards and plasma specimens with both assays. With Roche, Bland-Altman analysis showed bias of −0.3 and 95% limits of agreement of −2.6 to 1.8 log10, with 97/99 cases (97.9%) within agreement limits. With Abbott, Bland-Altman analysis showed bias of −0.1 and 95% limits of agreement of −2.3 to 2.1 log10, with 96/99 cases (96.9%) within agreement limits. Conclusion Our study demonstrated the feasibility of using FTA cards filter paper for HIV-1 viral load testing. However, further studies are required for FTA cards filter paper validation in HIV-1 treatment monitoring." @default.
• W2971325685 created "2019-09-05" @default.
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• W2971325685 date "2019-08-28" @default.
• W2971325685 modified "2023-09-27" @default.
• W2971325685 title "Whatman FTA®cards versus plasma specimens for the quantitation of HIV-1 RNA using two Real-Time PCR assays" @default.
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• W2971325685 doi "https://doi.org/10.1101/748061" @default.
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