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- W2971359501 abstract "To investigate the potential signaling pathway that regulates the proliferation of human CD34+ cells stimulated by prostaglandin E2 receptor 4 agonist (EP4A) in vitro.Twenty samples of peripheral blood containing stem cells were collected from the G-CSF mobilized healthy donors in our department of hematology. Human CD34+ cells were isolated by magnetic activated cell sorting (MACS) microbeads kit. The Cell Counting Kit-8 (CCK8) assay was used to determine the optimal concentration and time of EP4A to promote human CD34+ cell proliferation in vitro. Under the optimal condition, quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect mRNA level of β-catenin, and Western blot was used to assay protein expression of β-catenin and P-GSK-3β in human CD34+ cells treated with EP4A.Culturing with 10 µmol/L EP4A for 72 h, it was found that EP4A promoted human CD34+ cell proliferation significantly, and the proliferation rate of human CD34+ cells was 1.36 times higher than that of the control(P=0.002). Under the optimal condition, it was also found that EP4A enhanced the β-catenin expression at both mRNA and protein levels, and up-regulated phosphorylation of GSK-3β in human CD34+ cells, but these effects could be inhibited by the EP4A antagonist EP4AA.EP4A can enhance human CD34+ cell proliferation in vitro by activating Wnt/β-catenin signaling pathway." @default.
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- W2971359501 date "2017-06-01" @default.
- W2971359501 modified "2023-10-18" @default.
- W2971359501 title "[Prostaglandin E2 Receptor 4 Agonist Promotes Human CD34+ Cell Proliferation in vitro by Activating Wnt/β-Catenin Signaling Pathway]." @default.
- W2971359501 doi "https://doi.org/10.7534/j.issn.1009-2137.2017.03.004" @default.
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