FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2971386319> ?p ?o ?g. }

• W2971386319 endingPage "12254" @default.
• W2971386319 startingPage "12246" @default.
• W2971386319 abstract "Native ambient mass spectrometry has the potential for simultaneous analysis of native protein structure and spatial distribution within thin tissue sections. Notwithstanding sensitivity, this information can, in principle, be obtained for any protein present with no requirement for a priori knowledge of protein identity. To date, native ambient mass spectrometry has primarily made use of the liquid extraction surface analysis (LESA) sampling technique. Here, we address a fundamental question: Are the protein structures observed following native liquid extraction surface analysis representative of the protein structures within the substrate, or does the extraction process facilitate refolding (or unfolding)? Specifically, our aim was to determine whether protein-ligand complexes observed following LESA are indicative of complexes present in the substrate, or an artifact of the sampling process. The systems investigated were myoglobin and its noncovalently bound heme cofactor, and the Zn-binding protein carbonic anhydrase and its binding with ethoxzolamide. Charge state distributions, drift time profiles, and collision cross sections were determined by liquid extraction surface analysis ion mobility mass spectrometry of native and denatured proteins and compared with those obtained by direct infusion electrospray. The results show that it was not possible to refold denatured proteins with concomitant ligand binding (neither heme, zinc, nor ethoxzolamide) simply by use of native-like LESA solvents. That is, protein-ligand complexes were only observed by LESA MS when present in the substrate." @default.
• W2971386319 created "2019-09-12" @default.
• W2971386319 creator A5014883632 @default.
• W2971386319 creator A5089140177 @default.
• W2971386319 date "2019-09-06" @default.
• W2971386319 modified "2023-10-18" @default.
• W2971386319 title "Probing the Fundamentals of Native Liquid Extraction Surface Analysis Mass Spectrometry of Proteins: Can Proteins Refold during Extraction?" @default.
• W2971386319 cites W1496421513 @default.
• W2971386319 cites W1531583716 @default.
• W2971386319 cites W1898146877 @default.
• W2971386319 cites W1970317744 @default.
• W2971386319 cites W1972334042 @default.
• W2971386319 cites W1974704181 @default.
• W2971386319 cites W1974929504 @default.
• W2971386319 cites W1975860187 @default.
• W2971386319 cites W1983785284 @default.
• W2971386319 cites W1988308498 @default.
• W2971386319 cites W1988332348 @default.
• W2971386319 cites W1999616324 @default.
• W2971386319 cites W2012506672 @default.
• W2971386319 cites W2019175962 @default.
• W2971386319 cites W2019812098 @default.
• W2971386319 cites W2035192734 @default.
• W2971386319 cites W2035212554 @default.
• W2971386319 cites W2035725767 @default.
• W2971386319 cites W2037353814 @default.
• W2971386319 cites W2042198563 @default.
• W2971386319 cites W2049239108 @default.
• W2971386319 cites W2053702459 @default.
• W2971386319 cites W2065843920 @default.
• W2971386319 cites W2065890395 @default.
• W2971386319 cites W2074171925 @default.
• W2971386319 cites W2075881032 @default.
• W2971386319 cites W2088246435 @default.
• W2971386319 cites W2090553315 @default.
• W2971386319 cites W2095387019 @default.
• W2971386319 cites W2101303489 @default.
• W2971386319 cites W2114557793 @default.
• W2971386319 cites W2118336895 @default.
• W2971386319 cites W2121986660 @default.
• W2971386319 cites W2122570878 @default.
• W2971386319 cites W2124168908 @default.
• W2971386319 cites W2132182129 @default.
• W2971386319 cites W2132528537 @default.
• W2971386319 cites W2132629607 @default.
• W2971386319 cites W2148072075 @default.
• W2971386319 cites W2171506785 @default.
• W2971386319 cites W2186736649 @default.
• W2971386319 cites W2236752070 @default.
• W2971386319 cites W2316249707 @default.
• W2971386319 cites W2317233863 @default.
• W2971386319 cites W2328804008 @default.
• W2971386319 cites W2334789796 @default.
• W2971386319 cites W2335010019 @default.
• W2971386319 cites W2335585672 @default.
• W2971386319 cites W2418129919 @default.
• W2971386319 cites W2470396782 @default.
• W2971386319 cites W2505793428 @default.
• W2971386319 cites W2517160828 @default.
• W2971386319 cites W2521969918 @default.
• W2971386319 cites W2604980447 @default.
• W2971386319 cites W2732290919 @default.
• W2971386319 cites W2757211861 @default.
• W2971386319 cites W2761619919 @default.
• W2971386319 cites W2766617225 @default.
• W2971386319 cites W2770106397 @default.
• W2971386319 cites W2771474776 @default.
• W2971386319 cites W2782771962 @default.
• W2971386319 cites W2790000551 @default.
• W2971386319 cites W2792186809 @default.
• W2971386319 cites W2897772965 @default.
• W2971386319 cites W2910553840 @default.
• W2971386319 cites W2913182573 @default.
• W2971386319 cites W2914829148 @default.
• W2971386319 cites W2930405962 @default.
• W2971386319 cites W2940222661 @default.
• W2971386319 cites W1923871866 @default.
• W2971386319 doi "https://doi.org/10.1021/acs.analchem.9b02075" @default.
• W2971386319 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7006963" @default.
• W2971386319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31490666" @default.
• W2971386319 hasPublicationYear "2019" @default.
• W2971386319 type Work @default.
• W2971386319 sameAs 2971386319 @default.
• W2971386319 citedByCount "7" @default.
• W2971386319 countsByYear W29713863192020 @default.
• W2971386319 countsByYear W29713863192021 @default.
• W2971386319 countsByYear W29713863192022 @default.
• W2971386319 countsByYear W29713863192023 @default.
• W2971386319 crossrefType "journal-article" @default.
• W2971386319 hasAuthorship W2971386319A5014883632 @default.
• W2971386319 hasAuthorship W2971386319A5089140177 @default.
• W2971386319 hasBestOaLocation W29713863191 @default.
• W2971386319 hasConcept C111368507 @default.
• W2971386319 hasConcept C113196181 @default.
• W2971386319 hasConcept C116569031 @default.
• W2971386319 hasConcept C127313418 @default.
• W2971386319 hasConcept C15213710 @default.
• W2971386319 hasConcept C162356407 @default.

• next