FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2971562017> ?p ?o ?g. }

• W2971562017 endingPage "4338" @default.
• W2971562017 startingPage "4319" @default.
• W2971562017 abstract "Excipients are substances that are added to therapeutic products to improve stability, bioavailability, and manufacturability. Undesirable protein-protein interactions (PPI) can lead to self-association and/or high solution viscosity in concentrated protein formulations that are typically greater than 50 mg/mL. Therefore, understanding the effects of excipients on nonspecific PPI is important for more efficient formulation development. In this study, we used National Institute of Standards and Technology monoclonal antibody (NISTmAb) reference material as a model antibody protein to examine the physical stability and viscosity of concentrated formulations using a series of excipients, by varying pH, salt composition, and the presence of cosolutes including amino acids, sugars, and nonionic surfactants. Small angle X-ray scattering (SAXS) together with differential scanning calorimetry (DSC), dynamic light scattering (DLS), and viscosity measurements were used to obtain various experimental parameters to characterize excipient modulated PPI and bulk solution viscosities. In particular, a good correlation was found between SAXS and DLS/SLS results, suggesting that the use of DLS/SLS is valid for predicting the colloidal stability of NISTmAb in concentrated solutions. Moreover, further analysis of effective structure factor S(q)eff measured from SAXS enabled the dissection of net PPI into hydrodynamic forces due to excluded volume as well as any additional attractive or repulsive interactions with the presence of excipients. It was found that although no denaturation or aggregation of NISTmAb was observed and that the net PPI were repulsive, the use of ionic excipients such as pH and salts leads to increased short-range attraction, whereas the nonionic excipients including sugars, amino acids, and polysorbate surfactants lead to increased repulsive PPI with increasing protein concentration. Results obtained from viscosity measurements showed that the use of excipients can lead to increased solution viscosities at high protein concentrations. The use of S(q)eff, interaction parameter kD, and second virial coefficient B22 as predictors for solution viscosity was also evaluated by comparing the predicted results with the measured viscosities. Although B22 and S(q)eff appeared to be better predictors than kD, disagreement between the predicted and measured results suggests other factors apart from PPI contribute to the bulk rheological properties of concentrated protein solutions." @default.
• W2971562017 created "2019-09-12" @default.
• W2971562017 creator A5010533471 @default.
• W2971562017 creator A5018380045 @default.
• W2971562017 creator A5048647002 @default.
• W2971562017 creator A5072899505 @default.
• W2971562017 creator A5076403584 @default.
• W2971562017 date "2019-09-05" @default.
• W2971562017 modified "2023-10-07" @default.
• W2971562017 title "Studying Excipient Modulated Physical Stability and Viscosity of Monoclonal Antibody Formulations Using Small-Angle Scattering" @default.
• W2971562017 cites W1548932700 @default.
• W2971562017 cites W1832142381 @default.
• W2971562017 cites W1959907900 @default.
• W2971562017 cites W1971212417 @default.
• W2971562017 cites W1971234134 @default.
• W2971562017 cites W1972260684 @default.
• W2971562017 cites W1976268896 @default.
• W2971562017 cites W1982126789 @default.
• W2971562017 cites W1986936802 @default.
• W2971562017 cites W1987690443 @default.
• W2971562017 cites W1987695922 @default.
• W2971562017 cites W1989419578 @default.
• W2971562017 cites W1992470331 @default.
• W2971562017 cites W1997449658 @default.
• W2971562017 cites W2000621110 @default.
• W2971562017 cites W2000747979 @default.
• W2971562017 cites W2001171778 @default.
• W2971562017 cites W2003684685 @default.
• W2971562017 cites W2005157268 @default.
• W2971562017 cites W2006806491 @default.
• W2971562017 cites W2007450029 @default.
• W2971562017 cites W2008675038 @default.
• W2971562017 cites W2012434595 @default.
• W2971562017 cites W2013827851 @default.
• W2971562017 cites W2019456940 @default.
• W2971562017 cites W2024027867 @default.
• W2971562017 cites W2024064076 @default.
• W2971562017 cites W2026828177 @default.
• W2971562017 cites W2029314929 @default.
• W2971562017 cites W2029443601 @default.
• W2971562017 cites W2032556503 @default.
• W2971562017 cites W2036587914 @default.
• W2971562017 cites W2036879738 @default.
• W2971562017 cites W2041706350 @default.
• W2971562017 cites W2051527071 @default.
• W2971562017 cites W2051987035 @default.
• W2971562017 cites W2053591536 @default.
• W2971562017 cites W2053727980 @default.
• W2971562017 cites W2054187364 @default.
• W2971562017 cites W2056925186 @default.
• W2971562017 cites W2062214242 @default.
• W2971562017 cites W2063781503 @default.
• W2971562017 cites W2067848480 @default.
• W2971562017 cites W2069948193 @default.
• W2971562017 cites W2071226935 @default.
• W2971562017 cites W2072398283 @default.
• W2971562017 cites W2080538147 @default.
• W2971562017 cites W2087497084 @default.
• W2971562017 cites W2095256587 @default.
• W2971562017 cites W2096785891 @default.
• W2971562017 cites W2098361825 @default.
• W2971562017 cites W2100431233 @default.
• W2971562017 cites W2111488320 @default.
• W2971562017 cites W2122203160 @default.
• W2971562017 cites W2122676372 @default.
• W2971562017 cites W2130480376 @default.
• W2971562017 cites W2131692566 @default.
• W2971562017 cites W2142760719 @default.
• W2971562017 cites W2160176704 @default.
• W2971562017 cites W2162942780 @default.
• W2971562017 cites W2168193821 @default.
• W2971562017 cites W2181527856 @default.
• W2971562017 cites W2219759288 @default.
• W2971562017 cites W2229692518 @default.
• W2971562017 cites W2319652185 @default.
• W2971562017 cites W2320055325 @default.
• W2971562017 cites W2326344583 @default.
• W2971562017 cites W2326593475 @default.
• W2971562017 cites W2328316876 @default.
• W2971562017 cites W2335217992 @default.
• W2971562017 cites W2346096618 @default.
• W2971562017 cites W2405691703 @default.
• W2971562017 cites W2502470795 @default.
• W2971562017 cites W2513745190 @default.
• W2971562017 cites W2530313185 @default.
• W2971562017 cites W2530538317 @default.
• W2971562017 cites W2547172962 @default.
• W2971562017 cites W2549947033 @default.
• W2971562017 cites W2551210141 @default.
• W2971562017 cites W2563526210 @default.
• W2971562017 cites W2565843771 @default.
• W2971562017 cites W2587584959 @default.
• W2971562017 cites W2605404133 @default.
• W2971562017 cites W2607732975 @default.
• W2971562017 cites W2614728412 @default.
• W2971562017 cites W2615722084 @default.
• W2971562017 cites W2707646777 @default.
• W2971562017 cites W2725756931 @default.

• next