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• W2971775892 abstract "Abstract Food production in green crops is severely limited by low activity and poor specificity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in natural photosynthesis (NPS). This work presents a scientific solution to overcome this problem by immobilizing RuBisCO into a microfluidic reactor, which demonstrates a continuous production of glucose precursor at 13.8 μmol g −1 RuBisCO min −1 from CO 2 and ribulose-1,5-bisphosphate. Experiments show that the RuBisCO immobilization significantly enhances enzyme stabilities (7.2 folds in storage stability, 6.7 folds in thermal stability), and also improves the reusability (90.4% activity retained after 5 cycles of reuse and 78.5% after 10 cycles). This work mimics the NPS pathway with scalable microreactors for continuous synthesis of glucose precursor using very small amount of RuBisCO. Although still far from industrial production, this work demonstrates artificial synthesis of basic food materials by replicating the light-independent reactions of NPS, which may hold the key to food crisis relief and future space colonization." @default.
• W2971775892 created "2019-09-12" @default.
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• W2971775892 date "2019-09-06" @default.
• W2971775892 modified "2023-10-16" @default.
• W2971775892 title "Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors" @default.
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• W2971775892 doi "https://doi.org/10.1038/s41467-019-12089-6" @default.
• W2971775892 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6731257" @default.
• W2971775892 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31492867" @default.
• W2971775892 hasPublicationYear "2019" @default.
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