FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2971906608> ?p ?o ?g. }

• W2971906608 endingPage "120321" @default.
• W2971906608 startingPage "120321" @default.
• W2971906608 abstract "An optical aptasensor-based sensing platform for rapid insulin detection was fabricated. Aminated porous silica microparticles (PSiMPs) were synthesized via a facile mini-emulsion method to provide large surface area for covalent immobilization of insulin-binding DNA aptamer (IGA3) by glutaraldehyde cross-linking protocol. A Nickel-salphen type complex with piperidine side chain [Ni(II)-SP] was synthesized with a simple one-pot reaction, and functionalized as an optical label due to strong π-π interaction between aromatic carbons of G-quadruplex DNA aptamer and planar aromatic groups of Ni(II)-SP to form the immobilized IGA3-Ni(II)-SP complex, i.e. the dye-labeled aptamer, thereby bringing yellow colouration to the immobilized G-quartet plane. Optical characterization of aptasensor towards insulin binding was carried out with a fiber optic reflectance spectrophotometer. The maximum reflectance intensity of the immobilized IGA3-Ni(II)-SP complex at 656 nm decreased upon binding with insulin as aptasensor changed to brownish orange colouration in the background. This allows optical detection of insulin as the colour change of aptasensor is dependent on the insulin concentration. The linear detection range of the aptasensor is obtained from 10 to 50 μIU mL-1 (R2 = 0.9757), which conformed to the normal fasting insulin levels in human with a limit of detection (LOD) at 3.71 μIU mL-1. The aptasensor showed fast response time of 40 min and long shelf life stability of >3 weeks. Insulin detection using healthy human serums with informed consent provided by participants suggests the DNA aptamer biosensor was in good agreement with ELISA standard method using BIOMATIK Human INS (Insulin) ELISA Kit." @default.
• W2971906608 created "2019-09-12" @default.
• W2971906608 creator A5017005677 @default.
• W2971906608 creator A5031383187 @default.
• W2971906608 creator A5042169801 @default.
• W2971906608 creator A5050230952 @default.
• W2971906608 creator A5076641583 @default.
• W2971906608 creator A5080793061 @default.
• W2971906608 date "2020-01-01" @default.
• W2971906608 modified "2023-10-02" @default.
• W2971906608 title "Reflectance aptasensor based on metal salphen label for rapid and facile determination of insulin" @default.
• W2971906608 cites W1496037649 @default.
• W2971906608 cites W1976653675 @default.
• W2971906608 cites W1978765094 @default.
• W2971906608 cites W1980461645 @default.
• W2971906608 cites W1989226481 @default.
• W2971906608 cites W1993603706 @default.
• W2971906608 cites W2011462509 @default.
• W2971906608 cites W2013667183 @default.
• W2971906608 cites W2029538576 @default.
• W2971906608 cites W2034513011 @default.
• W2971906608 cites W2055775315 @default.
• W2971906608 cites W2057954591 @default.
• W2971906608 cites W2124226250 @default.
• W2971906608 cites W2138141188 @default.
• W2971906608 cites W2152055606 @default.
• W2971906608 cites W2253014186 @default.
• W2971906608 cites W2281860958 @default.
• W2971906608 cites W2334830949 @default.
• W2971906608 cites W2410439438 @default.
• W2971906608 cites W2525035163 @default.
• W2971906608 cites W2588461718 @default.
• W2971906608 cites W2615053500 @default.
• W2971906608 cites W2740524153 @default.
• W2971906608 cites W2769330637 @default.
• W2971906608 cites W2939361711 @default.
• W2971906608 doi "https://doi.org/10.1016/j.talanta.2019.120321" @default.
• W2971906608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31594568" @default.
• W2971906608 hasPublicationYear "2020" @default.
• W2971906608 type Work @default.
• W2971906608 sameAs 2971906608 @default.
• W2971906608 citedByCount "16" @default.
• W2971906608 countsByYear W29719066082020 @default.
• W2971906608 countsByYear W29719066082021 @default.
• W2971906608 countsByYear W29719066082022 @default.
• W2971906608 countsByYear W29719066082023 @default.
• W2971906608 crossrefType "journal-article" @default.
• W2971906608 hasAuthorship W2971906608A5017005677 @default.
• W2971906608 hasAuthorship W2971906608A5031383187 @default.
• W2971906608 hasAuthorship W2971906608A5042169801 @default.
• W2971906608 hasAuthorship W2971906608A5050230952 @default.
• W2971906608 hasAuthorship W2971906608A5076641583 @default.
• W2971906608 hasAuthorship W2971906608A5080793061 @default.
• W2971906608 hasConcept C108597893 @default.
• W2971906608 hasConcept C119128265 @default.
• W2971906608 hasConcept C120665830 @default.
• W2971906608 hasConcept C121332964 @default.
• W2971906608 hasConcept C128972844 @default.
• W2971906608 hasConcept C134018914 @default.
• W2971906608 hasConcept C13965031 @default.
• W2971906608 hasConcept C160756335 @default.
• W2971906608 hasConcept C185592680 @default.
• W2971906608 hasConcept C2777529624 @default.
• W2971906608 hasConcept C2779306644 @default.
• W2971906608 hasConcept C43617362 @default.
• W2971906608 hasConcept C54355233 @default.
• W2971906608 hasConcept C55493867 @default.
• W2971906608 hasConcept C71924100 @default.
• W2971906608 hasConcept C86803240 @default.
• W2971906608 hasConceptScore W2971906608C108597893 @default.
• W2971906608 hasConceptScore W2971906608C119128265 @default.
• W2971906608 hasConceptScore W2971906608C120665830 @default.
• W2971906608 hasConceptScore W2971906608C121332964 @default.
• W2971906608 hasConceptScore W2971906608C128972844 @default.
• W2971906608 hasConceptScore W2971906608C134018914 @default.
• W2971906608 hasConceptScore W2971906608C13965031 @default.
• W2971906608 hasConceptScore W2971906608C160756335 @default.
• W2971906608 hasConceptScore W2971906608C185592680 @default.
• W2971906608 hasConceptScore W2971906608C2777529624 @default.
• W2971906608 hasConceptScore W2971906608C2779306644 @default.
• W2971906608 hasConceptScore W2971906608C43617362 @default.
• W2971906608 hasConceptScore W2971906608C54355233 @default.
• W2971906608 hasConceptScore W2971906608C55493867 @default.
• W2971906608 hasConceptScore W2971906608C71924100 @default.
• W2971906608 hasConceptScore W2971906608C86803240 @default.
• W2971906608 hasFunder F4320322699 @default.
• W2971906608 hasLocation W29719066081 @default.
• W2971906608 hasLocation W29719066082 @default.
• W2971906608 hasOpenAccess W2971906608 @default.
• W2971906608 hasPrimaryLocation W29719066081 @default.
• W2971906608 hasRelatedWork W1988137149 @default.
• W2971906608 hasRelatedWork W2061135677 @default.
• W2971906608 hasRelatedWork W2095614141 @default.
• W2971906608 hasRelatedWork W2130341461 @default.
• W2971906608 hasRelatedWork W2278599837 @default.
• W2971906608 hasRelatedWork W2378104911 @default.
• W2971906608 hasRelatedWork W2748856529 @default.
• W2971906608 hasRelatedWork W2914720578 @default.

• next