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- W2972147604 abstract "Gold nanoparticles (AuNPs) bound with biomolecules have emerged as suitable biosensors exploiting unique surface chemistries and optical properties. Many efforts have focused on antibody bioconjugation to AuNPs resulting in a sensitive bioconjugate to detect specific types of bacteria. Unfortunately, bacteria thrive under various harsh environments, and an understanding of bioconjugate stability is needed. Here, we show a method for optimizing Listeria monocytogenes polyclonal antibodies bioconjugation mechanisms to AuNPs via covalent binding at different pH values, from 2 to 11, and 2-(N-morpholino)ethanesulfonic acid (MES), 3-(N-morpholino)propanesulfonic acid, NaOH, HCl conditions. By fitting Lorentz curves to the amide I and II regions, we analyze the stability of the antibody secondary structure. This shows an increase in the apparent breakdown of the antibody secondary structure during bioconjugation as pH decreases from 7.9 to 2. We find variable adsorption efficiency, measured as the percentage of antibody adsorbed to the AuNP surface, from 17 to 27% as pH increases from 2 to 6 before decreasing to 8 and 13% at pH 7.9 and 11, respectively. Transmission electron microscopy (TEM) analysis reveals discrepancies between size and morphological changes due to the corona layer assembly from antibody binding to single nanoparticles versus aggregation or cluster self-assembly into large aggregates. The corona layer formation size increases from 3.9 to 5.1 nm from pH 2 to 6, at pH 7.9, there is incomplete corona formation, whereas at pH 11, there is a corona layer formed of 6.4 nm. These results indicate that the covalent binding process was more efficient at lower pH values; however, aggregation and deactivation of the antibodies were observed. We demonstrate that optimum bioconjugation condition was determined at pH 6 and MES buffer-type by indicators of covalent bonding and stability of the antibody secondary structure using Fourier transform-infrared, the morphological characteristics and corona layer formation using TEM, and low wavelength shifts of ultraviolet–visible after bioconjugation." @default.
- W2972147604 created "2019-09-12" @default.
- W2972147604 creator A5025417159 @default.
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- W2972147604 creator A5068902661 @default.
- W2972147604 creator A5076025970 @default.
- W2972147604 date "2019-09-09" @default.
- W2972147604 modified "2023-10-14" @default.
- W2972147604 title "Optimization and Structural Stability of Gold Nanoparticle–Antibody Bioconjugates" @default.
- W2972147604 cites W1577364508 @default.
- W2972147604 cites W1940922490 @default.
- W2972147604 cites W1950921319 @default.
- W2972147604 cites W1964353134 @default.
- W2972147604 cites W1967943800 @default.
- W2972147604 cites W1976226355 @default.
- W2972147604 cites W1979445265 @default.
- W2972147604 cites W1986849434 @default.
- W2972147604 cites W1987205935 @default.
- W2972147604 cites W1989461197 @default.
- W2972147604 cites W1990379353 @default.
- W2972147604 cites W1992045220 @default.
- W2972147604 cites W1997089367 @default.
- W2972147604 cites W2001927093 @default.
- W2972147604 cites W2006077608 @default.
- W2972147604 cites W2006975013 @default.
- W2972147604 cites W2009562525 @default.
- W2972147604 cites W2010477422 @default.
- W2972147604 cites W2011705292 @default.
- W2972147604 cites W2012746817 @default.
- W2972147604 cites W2016323922 @default.
- W2972147604 cites W2019253511 @default.
- W2972147604 cites W2022180924 @default.
- W2972147604 cites W2024424036 @default.
- W2972147604 cites W2025585571 @default.
- W2972147604 cites W2029053759 @default.
- W2972147604 cites W2035872623 @default.
- W2972147604 cites W2037087528 @default.
- W2972147604 cites W2041065272 @default.
- W2972147604 cites W2042599500 @default.
- W2972147604 cites W2054022095 @default.
- W2972147604 cites W2054386280 @default.
- W2972147604 cites W2063551886 @default.
- W2972147604 cites W2064608337 @default.
- W2972147604 cites W2066943809 @default.
- W2972147604 cites W2070081261 @default.
- W2972147604 cites W2077231187 @default.
- W2972147604 cites W2079094085 @default.
- W2972147604 cites W2081444927 @default.
- W2972147604 cites W2087630244 @default.
- W2972147604 cites W2088107416 @default.
- W2972147604 cites W2094085775 @default.
- W2972147604 cites W2094463183 @default.
- W2972147604 cites W2102231003 @default.
- W2972147604 cites W2107735396 @default.
- W2972147604 cites W2113172577 @default.
- W2972147604 cites W2117504073 @default.
- W2972147604 cites W2120238718 @default.
- W2972147604 cites W2145279517 @default.
- W2972147604 cites W2145937569 @default.
- W2972147604 cites W2147982620 @default.
- W2972147604 cites W2149632285 @default.
- W2972147604 cites W2150244588 @default.
- W2972147604 cites W2159426997 @default.
- W2972147604 cites W2162447121 @default.
- W2972147604 cites W2163756710 @default.
- W2972147604 cites W2165669206 @default.
- W2972147604 cites W2239491650 @default.
- W2972147604 cites W2323152708 @default.
- W2972147604 cites W2327882671 @default.
- W2972147604 cites W2342343356 @default.
- W2972147604 cites W2343999888 @default.
- W2972147604 cites W2529692894 @default.
- W2972147604 cites W2531046423 @default.
- W2972147604 cites W2562806335 @default.
- W2972147604 cites W2581611120 @default.
- W2972147604 cites W2595421984 @default.
- W2972147604 cites W2597192241 @default.
- W2972147604 cites W2612081889 @default.
- W2972147604 cites W2766719126 @default.
- W2972147604 cites W2769123339 @default.
- W2972147604 cites W2811292748 @default.
- W2972147604 cites W2922485383 @default.
- W2972147604 cites W4238707347 @default.
- W2972147604 doi "https://doi.org/10.1021/acsomega.9b02276" @default.
- W2972147604 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6751724" @default.
- W2972147604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31552374" @default.
- W2972147604 hasPublicationYear "2019" @default.
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