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• W2972180526 startingPage "4303" @default.
• W2972180526 abstract "Seed development is an essential and complex process, which is involved in seed size change and various nutrients accumulation, and determines crop yield and quality. Common buckwheat (Fagopyrum esculentum Moench) is a widely cultivated minor crop with excellent economic and nutritional value in temperate zones. However, little is known about the molecular mechanisms of seed development in common buckwheat (Fagopyrum esculentum). In this study, we performed RNA-Seq to investigate the transcriptional dynamics and identify the key genes involved in common buckwheat seed development at three different developmental stages. A total of 4619 differentially expressed genes (DEGs) were identified. Based on the results of Gene Ontology (GO) and KEGG analysis of DEGs, many key genes involved in the seed development, including the Ca2+ signal transduction pathway, the hormone signal transduction pathways, transcription factors (TFs), and starch biosynthesis-related genes, were identified. More importantly, 18 DEGs were identified as the key candidate genes for seed size through homologous query using the known seed size-related genes from different seed plants. Furthermore, 15 DEGs from these identified as the key genes of seed development were selected to confirm the validity of the data by using quantitative real-time PCR (qRT-PCR), and the results show high consistency with the RNA-Seq results. Taken together, our results revealed the underlying molecular mechanisms of common buckwheat seed development and could provide valuable information for further studies, especially for common buckwheat seed improvement." @default.
• W2972180526 created "2019-09-12" @default.
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• W2972180526 date "2019-09-03" @default.
• W2972180526 modified "2023-10-17" @default.
• W2972180526 title "Transcriptome Analysis Reveals Key Seed-Development Genes in Common Buckwheat (Fagopyrum esculentum)" @default.
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• W2972180526 doi "https://doi.org/10.3390/ijms20174303" @default.
• W2972180526 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6747174" @default.
• W2972180526 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31484314" @default.
• W2972180526 hasPublicationYear "2019" @default.
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