Matches in SemOpenAlex for { <https://semopenalex.org/work/W2972207898> ?p ?o ?g. }
- W2972207898 endingPage "129422" @default.
- W2972207898 startingPage "129422" @default.
- W2972207898 abstract "Previous studies suggested that fibrillar human IAPP (hIAPP) is more likely to deposit in β-cells, resulting in β-cell injury. However, the changes in the conformation of hIAPP in lipid environment and the mechanism involved in β-cell damage are unclear.Synthetic hIAPP was incubated with five types of free fatty acids and phospholipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS), which constitute the cell membrane. Thioflavin-T fluorescence assay was conducted to analyze the degree of hIAPP fibrosis, and circular dichroism spectroscopy was performed to detect the β-fold formation of hIAPP. Furthermore, INS-1 cells were infected with human IAPP delivered by a GV230-EGFP plasmid. The effects of endogenous hIAPP overexpression induced by sodium palmitate on the survival, endoplasmic reticulum (ER) stress, and apoptosis of INS-1 cells were evaluated.The five types of free fatty acids can accelerate the fibrosis of hIAPP. Sodium palmitate also maintained the stability of fibrillar hIAPP. POPS, not POPC, accelerated hIAPP fibrosis. Treatment of INS-1 cells with sodium palmitate increased the expression of hIAPP, activated ER stress and ER stress-dependent apoptosis signaling pathways, and increased the apoptotic rate.Free fatty acids and anionic phospholipid can promote β-fold formation and fibrosis in hIAPP. High lipid induced the overexpression of hIAPP and aggravated ER stress and apoptosis in INS-1 cells, which caused β-cell death in high lipid environment.Our study reveals free fatty acids and hIAPP synergistically implicated in endoplasmic reticulum stress and apoptosis of islet β-cells." @default.
- W2972207898 created "2019-09-12" @default.
- W2972207898 creator A5004404242 @default.
- W2972207898 creator A5027392261 @default.
- W2972207898 creator A5032909562 @default.
- W2972207898 creator A5040654199 @default.
- W2972207898 creator A5049254402 @default.
- W2972207898 creator A5083118480 @default.
- W2972207898 creator A5090507089 @default.
- W2972207898 date "2020-01-01" @default.
- W2972207898 modified "2023-09-25" @default.
- W2972207898 title "Fibrillation of human islet amyloid polypeptide and its toxicity to pancreatic β-cells under lipid environment" @default.
- W2972207898 cites W1506165342 @default.
- W2972207898 cites W1511417959 @default.
- W2972207898 cites W1965562132 @default.
- W2972207898 cites W1966291084 @default.
- W2972207898 cites W1971419973 @default.
- W2972207898 cites W1981598554 @default.
- W2972207898 cites W1989089334 @default.
- W2972207898 cites W1989094428 @default.
- W2972207898 cites W1990468756 @default.
- W2972207898 cites W1994342334 @default.
- W2972207898 cites W2000483769 @default.
- W2972207898 cites W2001208905 @default.
- W2972207898 cites W2005745504 @default.
- W2972207898 cites W2014954984 @default.
- W2972207898 cites W2023850224 @default.
- W2972207898 cites W2034109892 @default.
- W2972207898 cites W2040377780 @default.
- W2972207898 cites W2054979846 @default.
- W2972207898 cites W2058493730 @default.
- W2972207898 cites W2068688814 @default.
- W2972207898 cites W2070114315 @default.
- W2972207898 cites W2072405906 @default.
- W2972207898 cites W2076991922 @default.
- W2972207898 cites W2082038580 @default.
- W2972207898 cites W2085933321 @default.
- W2972207898 cites W2089894176 @default.
- W2972207898 cites W2097259690 @default.
- W2972207898 cites W2101138844 @default.
- W2972207898 cites W2120764900 @default.
- W2972207898 cites W2125872855 @default.
- W2972207898 cites W2126926695 @default.
- W2972207898 cites W2145124130 @default.
- W2972207898 cites W2149315447 @default.
- W2972207898 cites W2153965694 @default.
- W2972207898 cites W2163584731 @default.
- W2972207898 cites W2334732111 @default.
- W2972207898 cites W2347074044 @default.
- W2972207898 cites W2357845632 @default.
- W2972207898 cites W2478248015 @default.
- W2972207898 cites W2516576960 @default.
- W2972207898 cites W2619988830 @default.
- W2972207898 cites W2744123648 @default.
- W2972207898 cites W2766028090 @default.
- W2972207898 cites W2789974412 @default.
- W2972207898 cites W2802049733 @default.
- W2972207898 cites W2807683823 @default.
- W2972207898 cites W2883950835 @default.
- W2972207898 cites W2888251066 @default.
- W2972207898 cites W2891590598 @default.
- W2972207898 cites W2901646965 @default.
- W2972207898 cites W2902812555 @default.
- W2972207898 cites W2903257335 @default.
- W2972207898 cites W2904223696 @default.
- W2972207898 cites W2912750849 @default.
- W2972207898 cites W2916384455 @default.
- W2972207898 cites W2922050669 @default.
- W2972207898 cites W2922202142 @default.
- W2972207898 cites W2939517625 @default.
- W2972207898 cites W4240479615 @default.
- W2972207898 cites W4240929891 @default.
- W2972207898 cites W4252957007 @default.
- W2972207898 doi "https://doi.org/10.1016/j.bbagen.2019.129422" @default.
- W2972207898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31491457" @default.
- W2972207898 hasPublicationYear "2020" @default.
- W2972207898 type Work @default.
- W2972207898 sameAs 2972207898 @default.
- W2972207898 citedByCount "6" @default.
- W2972207898 countsByYear W29722078982019 @default.
- W2972207898 countsByYear W29722078982020 @default.
- W2972207898 countsByYear W29722078982021 @default.
- W2972207898 crossrefType "journal-article" @default.
- W2972207898 hasAuthorship W2972207898A5004404242 @default.
- W2972207898 hasAuthorship W2972207898A5027392261 @default.
- W2972207898 hasAuthorship W2972207898A5032909562 @default.
- W2972207898 hasAuthorship W2972207898A5040654199 @default.
- W2972207898 hasAuthorship W2972207898A5049254402 @default.
- W2972207898 hasAuthorship W2972207898A5083118480 @default.
- W2972207898 hasAuthorship W2972207898A5090507089 @default.
- W2972207898 hasConcept C12554922 @default.
- W2972207898 hasConcept C139447449 @default.
- W2972207898 hasConcept C1491633281 @default.
- W2972207898 hasConcept C158617107 @default.
- W2972207898 hasConcept C179104552 @default.
- W2972207898 hasConcept C185592680 @default.
- W2972207898 hasConcept C190283241 @default.
- W2972207898 hasConcept C2777633098 @default.