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• W2972251157 endingPage "813" @default.
• W2972251157 startingPage "813" @default.
• W2972251157 abstract "Porcine circovirus type 2 (PCV2) is the etiological agent of PCV2-associated diseases (PCVAD). PCV2 targets lymphoblasts, and pigs suffering from PCVAD display lymphocyte depletion in lymphoid tissues. PCV2 infection of lymphoblasts has not been studied. Here, the replication cycle of PCV2 (abortion strain 1121 and PMWS strain Stoon1010) in T-lymphoblasts was examined. The expression of Rep and Cap were found for both viral strains, while progeny virus was detected for Stoon1010 but not for 1121. PCV2 attached to 11-26% (1121-Stoon1010) of the T-lymphoblasts while 2.6-12.7% of cells showed virus internalization. Chondroitin sulfate (CS) was present on 25% of T-lymphoblasts, and colocalized with PCV2 on 31-32% of the PCV2+ cells. Enzymatic removal of CS reduced PCV2 infection. PCV2 infection was decreased by chlorpromazine, cytochalasin D and Clostridium difficile toxin B for both viral strains and by amiloride for 1121 but not for Stoon1010. Inhibiting either endosome acidification or serine proteases strongly reduced PCV2 infection. Three-dimensional analysis of Cap structure demonstrated a better Cap-nucleic acid affinity for Stoon1010 than for 1121. Taken together, PCV2 binds to T-lymphoblasts partially via CS, enters via clathrin-mediated endocytosis, and disassembles under functions of a pH-drop and serine proteases. Strain Stoon1010 displayed an enhanced viral binding, a specific receptor-mediated endocytosis, an increased Cap-nucleic acid affinity, and a more productive infection in T-lymphoblasts than 1121 did, indicating an evolution from 1121 to Stoon1010." @default.
• W2972251157 created "2019-09-12" @default.
• W2972251157 creator A5012390211 @default.
• W2972251157 creator A5062878756 @default.
• W2972251157 creator A5089436970 @default.
• W2972251157 date "2019-09-02" @default.
• W2972251157 modified "2023-09-24" @default.
• W2972251157 title "Strain-Dependent Porcine Circovirus Type 2 (PCV2) Entry and Replication in T-Lymphoblasts" @default.
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• W2972251157 doi "https://doi.org/10.3390/v11090813" @default.
• W2972251157 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6783876" @default.
• W2972251157 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31480752" @default.
• W2972251157 hasPublicationYear "2019" @default.
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