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- W2972496797 endingPage "477" @default.
- W2972496797 startingPage "477" @default.
- W2972496797 abstract "There are a large number of biomolecules that are accountable for the extremely crowded intracellular environment, which is totally different from the dilute solutions, i.e., the idealized conditions. Such crowded environment due to the presence of macromolecules of different sizes, shapes, and composition governs the level of crowding inside a cell. Thus, we investigated the effect of different sizes and shapes of crowders (ficoll 70, dextran 70, and dextran 40), which are polysaccharide in nature, on the thermodynamic stability, structure, and functional activity of two model proteins using UV-Vis spectroscopy and circular dichroism techniques. We observed that (a) the extent of stabilization of α-lactalbumin and lysozyme increases with the increasing concentration of the crowding agents due to the excluded volume effect and the small-sized and rod-shaped crowder, i.e., dextran 40 resulted in greater stabilization of both proteins than dextran 70 and ficoll 70; (b) structure of both the proteins remains unperturbed; and (c) enzymatic activity of lysozyme decreases with the increasing concentration of the crowder." @default.
- W2972496797 created "2019-09-19" @default.
- W2972496797 creator A5021941753 @default.
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- W2972496797 creator A5066278286 @default.
- W2972496797 creator A5070363347 @default.
- W2972496797 creator A5077513922 @default.
- W2972496797 date "2019-09-12" @default.
- W2972496797 modified "2023-10-14" @default.
- W2972496797 title "Carbohydrate-Based Macromolecular Crowding-Induced Stabilization of Proteins: Towards Understanding the Significance of the Size of the Crowder" @default.
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