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• W2972605516 endingPage "110190" @default.
• W2972605516 startingPage "110190" @default.
• W2972605516 abstract "Plant secondary metabolites are valuable therapeutics not readily synthesized by traditional chemistry techniques. Although their enrichment in plant cell cultures is possible following advances in biotechnology, conventional methods of recovery are destructive to the tissues. Nanoharvesting, in which nanoparticles are designed to bind and carry biomolecules out of living cells, offers continuous production of metabolites from plant cultures. Here, nanoharvesting of polyphenolic flavonoids, model plant-derived therapeutics, enriched in Solidago nemoralis hairy root cultures, is performed using engineered mesoporous silica nanoparticles (MSNPs, 165 nm diameter and 950 m2/g surface area) functionalized with both titanium dioxide (TiO2, 425 mg/g particles) for coordination binding sites, and amines (NH2, 145 mg/g particles) to promote cellular internalization. Intracellular uptake and localization of the nanoparticles (in Murashige and Skoog media) in hairy roots were confirmed by tagging the particles with rhodamine B isothiocyanate, incubating the particles with hairy roots, and quenching bulk fluorescence using trypan blue. Nanoharvesting of biologically active flavonoids was demonstrated by observing increased antiradical activity (using 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay) by nanoparticles after exposure to hairy roots (indicating general antioxidant activity), and by the displacement of the radio-ligand [3H]-methyllycaconitine from rat hippocampal nicotinic receptors by solutes recovered from nanoharvested particles (indicating pharmacological activity specific to S. nemoralis flavonoids). Post-nanoharvesting growth suggests that the roots are viable after nanoharvesting, and capable of continued flavonoid synthesis. These observations demonstrate the potential for using engineered nanostructured particles to facilitate continuous isolation of a broad range of biomolecules from living and functioning plant cultures." @default.
• W2972605516 created "2019-09-19" @default.
• W2972605516 creator A5002041799 @default.
• W2972605516 creator A5008435015 @default.
• W2972605516 creator A5054023806 @default.
• W2972605516 creator A5055895630 @default.
• W2972605516 creator A5057131258 @default.
• W2972605516 creator A5063343761 @default.
• W2972605516 creator A5067113549 @default.
• W2972605516 date "2020-01-01" @default.
• W2972605516 modified "2023-09-30" @default.
• W2972605516 title "Nanoharvesting of bioactive materials from living plant cultures using engineered silica nanoparticles" @default.
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