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- W2972649069 abstract "Abstract Background Turmeric is commonly used as a dietary treatment for inflammation, but few studies have evaluated the direct effect of turmeric on cartilage. The purpose of this study was to characterize cartilage explants’ inflammatory responses to lipopolysaccharide in the presence of a simulated biological extract of turmeric. Methods Turmeric was incubated in simulated gastric and intestinal fluid, followed by inclusion of liver microsomes and NADPH. The resulting extract (TUR sim ) was used to condition cartilage explants in the presence or absence of lipopolysaccharide. Explants were cultured for 96 h (h); the first 24 h in basal tissue culture media and the remaining 72 h in basal tissue culture media containing TUR sim (0, 3, 9 or 15 μg/mL). Lipopolysaccharide (0 or 5 μg/mL) was added for the final 48 H . media samples were collected immediately prior to lipopolysaccharide exposure (0 h) and then at 24 and 48 h after, and analyzed for prostaglandin E 2 (PGE 2 ), glycosaminoglycan (GAG), and nitric oxide (NO). Explants were stained with calcein-AM for an estimate of live cells. Data were analyzed using a 2-way repeated measures (GAG, PGE 2 , NO) or 1-way ANOVA without repeated measures (viability). Significance accepted at p < 0.05. Results TUR sim significantly reduced PGE 2, NO and GAG, and calcein fluorescence was reduced. Conclusions: These data contribute to the growing body of evidence for the utility of turmeric as an intervention for cartilage inflammation." @default.
- W2972649069 created "2019-09-19" @default.
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- W2972649069 date "2019-09-11" @default.
- W2972649069 modified "2023-09-24" @default.
- W2972649069 title "A biological extract of turmeric (Curcuma longa) modulates response of cartilage explants to lipopolysaccharide" @default.
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- W2972649069 doi "https://doi.org/10.1186/s12906-019-2660-z" @default.
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