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• W2972694725 abstract "Abstract Objectives Chemotherapeutic drugs induce senescence in cancer cells but, unlike replicative senescence or oncogene‐induced senescence, do so rather inefficiently and depending on DNA damage. A thorough understanding of the biology of chemotherapy‐induced senescent cells requires their isolation from a mixed population of adjacent senescent and non‐senescent cancer cells. Materials and methods We have developed and optimized a rapid iodixanol (OptiPrep)‐based gradient centrifugation system to identify, isolate and characterize doxorubicin (DXR)‐induced senescent hepatocellular carcinoma (HCC) cells (HepG2 and Huh‐7) in vitro. Results After cellular exposure to DXR, we used iodixanol gradient‐based centrifugation to isolate and re‐plate cells on collagen‐coated flasks, despite their low or null proliferative capacity. The isolated cell populations were enriched for DXR‐induced senescent HCC cells, as confirmed by proliferation arrest assay, and β‐galactosidase and DNA damage‐dependent γH2A.X staining. Conclusions Analysing pure cultures of chemotherapy‐induced senescent versus non‐responsive cancer cells will increase our knowledge on chemotherapeutic mechanisms of action, and help refine current therapeutic strategies." @default.
• W2972694725 created "2019-09-19" @default.
• W2972694725 creator A5069528663 @default.
• W2972694725 creator A5083412665 @default.
• W2972694725 date "2019-09-13" @default.
• W2972694725 modified "2023-10-16" @default.
• W2972694725 title "Isolation of senescent cells by iodixanol (OptiPrep) density gradient‐based separation" @default.
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• W2972694725 doi "https://doi.org/10.1111/cpr.12674" @default.
• W2972694725 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6869531" @default.
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• W2972694725 hasPublicationYear "2019" @default.
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