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• W2973171421 endingPage "111702" @default.
• W2973171421 startingPage "111702" @default.
• W2973171421 abstract "Photoelectrochemical (PEC) assay with low background, simple instrumentation and high sensitivity has deemed as one of the most potential strategies to simultaneous multi-component detection. How to distinguish photocurrent changes caused by various targets on a single sensing platform thus becomes the key issue to be resolved. Herein, we innovatively proposed a multiplex PEC biosensor based on wavelength distinguishable signal quenching and enhancing toward photoactive material 3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA) for simultaneous assay of dual metal ions. Briefly, S1 and S2 ssDNA containing sensitizer methylene blue and quencher ferrocene (termed as MB-S1 and Fc-S2), respectively, were first generated through target Pb2+ and Mg2+-induced DNAzyme-assisted target recycling, which thereafter were modified on PTCDA sensing platform specifically via host-guest recognition with β-cyclodextrin (β-CD). Interestingly, the sensitizer MB could enhance photocurrent of PTCDA under the excitation wavelength of 623 nm and 590 nm, respectively, while the quencher Fc just quencher the photocurrent of PTCDA under the excitation wavelength of 590 nm, thereby achieving wavelength distinguishable signal quenching and enhancing toward photoactive material PTCDA for simultaneous assay of dual metal ions. As a result, the conceived biosensor for Mg2+ and Pb2+ detection realized high sensitivity with detection limit of 0.3 pM and 0.3 nM, respectively. The proposed strategy not only for the first time achieved the discrimination of varied PEC signal caused by two targets with usage of sole photoelectric material, but also realized the simultaneous multiplex assay on a single sensing platform, providing a new way for constructing effective and sensitive PEC biosensor for multi-component detection." @default.
• W2973171421 created "2019-09-19" @default.
• W2973171421 creator A5018452581 @default.
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• W2973171421 date "2019-12-01" @default.
• W2973171421 modified "2023-10-18" @default.
• W2973171421 title "Wavelength distinguishable signal quenching and enhancing toward photoactive material 3,4,9,10-perylenetetracarboxylic dianhydride for simultaneous assay of dual metal ions" @default.
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• W2973171421 doi "https://doi.org/10.1016/j.bios.2019.111702" @default.
• W2973171421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31561096" @default.
• W2973171421 hasPublicationYear "2019" @default.
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