FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2973611704> ?p ?o ?g. }

• W2973611704 endingPage "460554" @default.
• W2973611704 startingPage "460554" @default.
• W2973611704 abstract "Therapeutic antibodies can elicit an immune response through different mechanisms, either cell independent via complement activation (CDC) or through activation of immune-effector cells (such as macrophages and NK cells). After target binding, the Fc part of the antibody will interact with Fc receptors on the surface of effector cells, leading to activation and lysis of the target cells by a mechanism called antibody-dependent cell-mediated cytotoxicity (ADCC). The ADCC of an antibody can be increased by modifying the carbohydrates on the Fc part. If the fucose on the first N-acetylglucosamine is absent, the affinity for the FcγRIIIa is increased and the ADCC enhanced. We describe the development of a chromatography method that is based on the differential affinity of the Fc receptor FcγRIIIa (high affinity V158 variant) for fucosylated and a-fucosylated antibodies. Immobilized FcγRIIIa can be used for the separation of immunoglobulins carrying these glycosylation variants for both, analytical and preparative purposes. The biological activity and fucose content of three pools enriched for fully fucosylated, mono-fucosylated or a-fucosylated carbohydrates could be characterized. Mono-fucosylated and a-fucosylated immunoglobulins have the same enhanced biological activity compared to fully fucosylated IgGs. A direct, label- and modification-free analytical method for screening of IgGs from culture supernatant was developed and was amenable to high-throughput screening. Clones producing antibodies with a high content of a-fucosylated oligosaccharides could be successfully selected." @default.
• W2973611704 created "2019-09-26" @default.
• W2973611704 creator A5006906885 @default.
• W2973611704 creator A5013013933 @default.
• W2973611704 creator A5028900839 @default.
• W2973611704 creator A5040318787 @default.
• W2973611704 creator A5056556748 @default.
• W2973611704 creator A5059660756 @default.
• W2973611704 creator A5073850898 @default.
• W2973611704 creator A5091501524 @default.
• W2973611704 date "2020-01-01" @default.
• W2973611704 modified "2023-09-25" @default.
• W2973611704 title "FcγRIIIa chromatography to enrich a-fucosylated glycoforms and assess the potency of glycoengineered therapeutic antibodies" @default.
• W2973611704 cites W1608505606 @default.
• W2973611704 cites W1969824500 @default.
• W2973611704 cites W2015723482 @default.
• W2973611704 cites W2018249974 @default.
• W2973611704 cites W2055400546 @default.
• W2973611704 cites W2062200963 @default.
• W2973611704 cites W2078490791 @default.
• W2973611704 cites W2079579628 @default.
• W2973611704 cites W2091057822 @default.
• W2973611704 cites W2100518445 @default.
• W2973611704 cites W2113529095 @default.
• W2973611704 cites W2115571948 @default.
• W2973611704 cites W2120319857 @default.
• W2973611704 cites W2136007354 @default.
• W2973611704 cites W2137354517 @default.
• W2973611704 cites W2143869160 @default.
• W2973611704 cites W2152155460 @default.
• W2973611704 cites W2190603070 @default.
• W2973611704 cites W2325475319 @default.
• W2973611704 cites W2335483660 @default.
• W2973611704 cites W2509039973 @default.
• W2973611704 cites W2769473277 @default.
• W2973611704 cites W2793677330 @default.
• W2973611704 cites W98754212 @default.
• W2973611704 doi "https://doi.org/10.1016/j.chroma.2019.460554" @default.
• W2973611704 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31597603" @default.
• W2973611704 hasPublicationYear "2020" @default.
• W2973611704 type Work @default.
• W2973611704 sameAs 2973611704 @default.
• W2973611704 citedByCount "8" @default.
• W2973611704 countsByYear W29736117042021 @default.
• W2973611704 countsByYear W29736117042022 @default.
• W2973611704 countsByYear W29736117042023 @default.
• W2973611704 crossrefType "journal-article" @default.
• W2973611704 hasAuthorship W2973611704A5006906885 @default.
• W2973611704 hasAuthorship W2973611704A5013013933 @default.
• W2973611704 hasAuthorship W2973611704A5028900839 @default.
• W2973611704 hasAuthorship W2973611704A5040318787 @default.
• W2973611704 hasAuthorship W2973611704A5056556748 @default.
• W2973611704 hasAuthorship W2973611704A5059660756 @default.
• W2973611704 hasAuthorship W2973611704A5073850898 @default.
• W2973611704 hasAuthorship W2973611704A5091501524 @default.
• W2973611704 hasBestOaLocation W29736117041 @default.
• W2973611704 hasConcept C108625454 @default.
• W2973611704 hasConcept C109316439 @default.
• W2973611704 hasConcept C125823703 @default.
• W2973611704 hasConcept C153911025 @default.
• W2973611704 hasConcept C159654299 @default.
• W2973611704 hasConcept C170493617 @default.
• W2973611704 hasConcept C179247698 @default.
• W2973611704 hasConcept C181199279 @default.
• W2973611704 hasConcept C185592680 @default.
• W2973611704 hasConcept C202751555 @default.
• W2973611704 hasConcept C203014093 @default.
• W2973611704 hasConcept C206212055 @default.
• W2973611704 hasConcept C2776841590 @default.
• W2973611704 hasConcept C2777313579 @default.
• W2973611704 hasConcept C2779212266 @default.
• W2973611704 hasConcept C2779931124 @default.
• W2973611704 hasConcept C40677261 @default.
• W2973611704 hasConcept C51785407 @default.
• W2973611704 hasConcept C55493867 @default.
• W2973611704 hasConcept C86803240 @default.
• W2973611704 hasConcept C8891405 @default.
• W2973611704 hasConceptScore W2973611704C108625454 @default.
• W2973611704 hasConceptScore W2973611704C109316439 @default.
• W2973611704 hasConceptScore W2973611704C125823703 @default.
• W2973611704 hasConceptScore W2973611704C153911025 @default.
• W2973611704 hasConceptScore W2973611704C159654299 @default.
• W2973611704 hasConceptScore W2973611704C170493617 @default.
• W2973611704 hasConceptScore W2973611704C179247698 @default.
• W2973611704 hasConceptScore W2973611704C181199279 @default.
• W2973611704 hasConceptScore W2973611704C185592680 @default.
• W2973611704 hasConceptScore W2973611704C202751555 @default.
• W2973611704 hasConceptScore W2973611704C203014093 @default.
• W2973611704 hasConceptScore W2973611704C206212055 @default.
• W2973611704 hasConceptScore W2973611704C2776841590 @default.
• W2973611704 hasConceptScore W2973611704C2777313579 @default.
• W2973611704 hasConceptScore W2973611704C2779212266 @default.
• W2973611704 hasConceptScore W2973611704C2779931124 @default.
• W2973611704 hasConceptScore W2973611704C40677261 @default.
• W2973611704 hasConceptScore W2973611704C51785407 @default.
• W2973611704 hasConceptScore W2973611704C55493867 @default.
• W2973611704 hasConceptScore W2973611704C86803240 @default.
• W2973611704 hasConceptScore W2973611704C8891405 @default.

• next