Matches in SemOpenAlex for { <https://semopenalex.org/work/W2974408974> ?p ?o ?g. }
- W2974408974 abstract "Abstract Macrophage-expressed gene 1 (MPEG1/Perforin-2) is a perforin-like protein that functions within the phagolysosome to damage engulfed microbes. MPEG1 is thought to form pores in target membranes, however, its mode of action remains unknown. We use cryo-Electron Microscopy (cryo-EM) to determine the 2.4 Å structure of a hexadecameric assembly of MPEG1 that displays the expected features of a soluble prepore complex. We further discover that MPEG1 prepore-like assemblies can be induced to perforate membranes through acidification, such as would occur within maturing phagolysosomes. We next solve the 3.6 Å cryo-EM structure of MPEG1 in complex with liposomes. These data reveal that a multi-vesicular body of 12 kDa (MVB12)-associated β-prism (MABP) domain binds membranes such that the pore-forming machinery of MPEG1 is oriented away from the bound membrane. This unexpected mechanism of membrane interaction suggests that MPEG1 remains bound to the phagolysosome membrane while simultaneously forming pores in engulfed bacterial targets." @default.
- W2974408974 created "2019-09-26" @default.
- W2974408974 creator A5001782977 @default.
- W2974408974 creator A5008315680 @default.
- W2974408974 creator A5008970099 @default.
- W2974408974 creator A5009119703 @default.
- W2974408974 creator A5012177640 @default.
- W2974408974 creator A5017320160 @default.
- W2974408974 creator A5022727654 @default.
- W2974408974 creator A5023000303 @default.
- W2974408974 creator A5025068841 @default.
- W2974408974 creator A5036417435 @default.
- W2974408974 creator A5046822952 @default.
- W2974408974 creator A5050620343 @default.
- W2974408974 creator A5067574520 @default.
- W2974408974 creator A5073289544 @default.
- W2974408974 creator A5076236652 @default.
- W2974408974 creator A5077923906 @default.
- W2974408974 creator A5083144855 @default.
- W2974408974 creator A5091682466 @default.
- W2974408974 date "2019-09-19" @default.
- W2974408974 modified "2023-10-10" @default.
- W2974408974 title "The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1" @default.
- W2974408974 cites W1155883168 @default.
- W2974408974 cites W1838872057 @default.
- W2974408974 cites W1979438194 @default.
- W2974408974 cites W1988779160 @default.
- W2974408974 cites W1989353671 @default.
- W2974408974 cites W2001276190 @default.
- W2974408974 cites W2011606192 @default.
- W2974408974 cites W2014951652 @default.
- W2974408974 cites W2018180763 @default.
- W2974408974 cites W2029667189 @default.
- W2974408974 cites W2030513906 @default.
- W2974408974 cites W2030517878 @default.
- W2974408974 cites W2031294950 @default.
- W2974408974 cites W2032146255 @default.
- W2974408974 cites W2034132228 @default.
- W2974408974 cites W2037559791 @default.
- W2974408974 cites W2045227909 @default.
- W2974408974 cites W2072973641 @default.
- W2974408974 cites W2089934202 @default.
- W2974408974 cites W2090821297 @default.
- W2974408974 cites W2093849705 @default.
- W2974408974 cites W2094375702 @default.
- W2974408974 cites W2100455255 @default.
- W2974408974 cites W2104130324 @default.
- W2974408974 cites W2104234755 @default.
- W2974408974 cites W2120396539 @default.
- W2974408974 cites W2124026197 @default.
- W2974408974 cites W2138150368 @default.
- W2974408974 cites W2140813750 @default.
- W2974408974 cites W2154714625 @default.
- W2974408974 cites W2159338660 @default.
- W2974408974 cites W2163246095 @default.
- W2974408974 cites W2171510183 @default.
- W2974408974 cites W2180357773 @default.
- W2974408974 cites W2258912434 @default.
- W2974408974 cites W2285587112 @default.
- W2974408974 cites W2346613028 @default.
- W2974408974 cites W2471671221 @default.
- W2974408974 cites W2584282544 @default.
- W2974408974 cites W2587625522 @default.
- W2974408974 cites W2593511418 @default.
- W2974408974 cites W2606710580 @default.
- W2974408974 cites W2728385286 @default.
- W2974408974 cites W2732730999 @default.
- W2974408974 cites W2734356283 @default.
- W2974408974 cites W2789819999 @default.
- W2974408974 cites W2791354025 @default.
- W2974408974 cites W2802099741 @default.
- W2974408974 cites W2886214535 @default.
- W2974408974 cites W2891482515 @default.
- W2974408974 cites W2899714098 @default.
- W2974408974 cites W2950224362 @default.
- W2974408974 cites W2951611668 @default.
- W2974408974 cites W2952729952 @default.
- W2974408974 cites W2953320394 @default.
- W2974408974 cites W2970462530 @default.
- W2974408974 cites W2986445122 @default.
- W2974408974 doi "https://doi.org/10.1038/s41467-019-12279-2" @default.
- W2974408974 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6753088" @default.
- W2974408974 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31537793" @default.
- W2974408974 hasPublicationYear "2019" @default.
- W2974408974 type Work @default.
- W2974408974 sameAs 2974408974 @default.
- W2974408974 citedByCount "60" @default.
- W2974408974 countsByYear W29744089742019 @default.
- W2974408974 countsByYear W29744089742020 @default.
- W2974408974 countsByYear W29744089742021 @default.
- W2974408974 countsByYear W29744089742022 @default.
- W2974408974 countsByYear W29744089742023 @default.
- W2974408974 crossrefType "journal-article" @default.
- W2974408974 hasAuthorship W2974408974A5001782977 @default.
- W2974408974 hasAuthorship W2974408974A5008315680 @default.
- W2974408974 hasAuthorship W2974408974A5008970099 @default.
- W2974408974 hasAuthorship W2974408974A5009119703 @default.
- W2974408974 hasAuthorship W2974408974A5012177640 @default.
- W2974408974 hasAuthorship W2974408974A5017320160 @default.
- W2974408974 hasAuthorship W2974408974A5022727654 @default.