FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2974568513> ?p ?o ?g. }

• W2974568513 endingPage "172" @default.
• W2974568513 startingPage "158" @default.
• W2974568513 abstract "Cell spheroids as building blocks for engineering micro-tissue should be able to mimic the complex structure of natural tissue. However, control of the distribution of multiple cell populations within cell spheroids is difficult to achieve with current spheroid-harvest methods such as hanging-drop and with the use of microwell plates. In this study, we report the fabrication of core-shell spheroids with the ultimate goal to form 3D complex micro-tissue. We used endothelial cells and two types of stem cells (human turbinate mesenchymal stem cells (hTMSCs)/adipose-derived stem cells (ADSCs)). The stem cells and endothelial cells formed layered micro-sized cell sheets (µCSs) on polydopamine micro-patterned temperature-responsive hydrogel surfaces by a sequential seeding method, and these layered µCSs self-assembled to form core-shell spheroids by expansion of the hydrogels. The co-cultured spheroids formed a core-shell structure irrespective of stem cell type. In addition, the size of the core-shell spheroids was controlled from 90 ± 1 to 144 ± 3 µm by changing pattern sizes (200, 300, and 400 µm). The shell thickness gradually increased from 12 ± 3 to 30 ± 6 µm by adjusting the endothelial cell seeding density. Finally, we fabricated the micro-tissue by fusion of the co-cultured spheroids, and the spheroids with the core-shell structure rapidly induced in vitro vessel-like network in 3 days. Thus, the position of endothelial cells in co-cultured spheroids may be an important factor for the modulation of the vascularization process, which can be useful for the production of 3D complex micro-tissues using spheroids as building blocks. This manuscript describes our work on the fabrication of core-shell spheroids as building blocks to form 3D complex vascularized micro-tissue. Stem cells (human turbinate mesenchymal stem cells (hTMSCs) or adipose-derived stem cells (ADSCs)) and endothelial cells formed layered micro-sized cell sheets (µCSs) on micro-patterned temperature-responsive hydrogel surfaces by a sequential seeding method, and these layered µCSs self-assembled to form core-shell spheroids (core – stem cells, shell – endothelial cells), irrespective of stem cell type. In addition, the size and shell thickness of the core-shell spheroids were controlled by modifying pattern size and endothelial cell seeding density. We fabricated the vascularized micro-tissue by fusion of the spheroids and demonstrated that the spheroids with a core-shell structure rapidly induced vessel-like network." @default.
• W2974568513 created "2019-09-26" @default.
• W2974568513 creator A5017387288 @default.
• W2974568513 creator A5021289768 @default.
• W2974568513 creator A5028314308 @default.
• W2974568513 creator A5041862198 @default.
• W2974568513 creator A5046146264 @default.
• W2974568513 creator A5058246995 @default.
• W2974568513 creator A5062317501 @default.
• W2974568513 creator A5080204034 @default.
• W2974568513 date "2019-12-01" @default.
• W2974568513 modified "2023-10-16" @default.
• W2974568513 title "Fabrication of core-shell spheroids as building blocks for engineering 3D complex vascularized tissue" @default.
• W2974568513 cites W1483526410 @default.
• W2974568513 cites W1574424832 @default.
• W2974568513 cites W1589561469 @default.
• W2974568513 cites W1965440553 @default.
• W2974568513 cites W1974040642 @default.
• W2974568513 cites W1975484052 @default.
• W2974568513 cites W1978854686 @default.
• W2974568513 cites W1989361067 @default.
• W2974568513 cites W1991073285 @default.
• W2974568513 cites W1996081149 @default.
• W2974568513 cites W2000841030 @default.
• W2974568513 cites W2006431507 @default.
• W2974568513 cites W2008447781 @default.
• W2974568513 cites W2008460052 @default.
• W2974568513 cites W2014105503 @default.
• W2974568513 cites W2016183704 @default.
• W2974568513 cites W2021011246 @default.
• W2974568513 cites W2030135968 @default.
• W2974568513 cites W2032527828 @default.
• W2974568513 cites W2034159130 @default.
• W2974568513 cites W2047424436 @default.
• W2974568513 cites W2048644480 @default.
• W2974568513 cites W2057507744 @default.
• W2974568513 cites W2084904115 @default.
• W2974568513 cites W2085112343 @default.
• W2974568513 cites W2091452743 @default.
• W2974568513 cites W2094041610 @default.
• W2974568513 cites W2094957208 @default.
• W2974568513 cites W2108578973 @default.
• W2974568513 cites W2123092754 @default.
• W2974568513 cites W2138717089 @default.
• W2974568513 cites W2149162919 @default.
• W2974568513 cites W2153202870 @default.
• W2974568513 cites W2154242323 @default.
• W2974568513 cites W2158017733 @default.
• W2974568513 cites W2163299187 @default.
• W2974568513 cites W2181514791 @default.
• W2974568513 cites W2203385592 @default.
• W2974568513 cites W2294204843 @default.
• W2974568513 cites W2298051556 @default.
• W2974568513 cites W2319562462 @default.
• W2974568513 cites W2334127123 @default.
• W2974568513 cites W2520900466 @default.
• W2974568513 cites W2578027109 @default.
• W2974568513 cites W2579977596 @default.
• W2974568513 cites W2589861178 @default.
• W2974568513 cites W2624368609 @default.
• W2974568513 cites W2755989260 @default.
• W2974568513 cites W2766832099 @default.
• W2974568513 cites W2789310625 @default.
• W2974568513 cites W2800187340 @default.
• W2974568513 cites W2905691564 @default.
• W2974568513 cites W4253263568 @default.
• W2974568513 cites W4361807767 @default.
• W2974568513 cites W748483617 @default.
• W2974568513 doi "https://doi.org/10.1016/j.actbio.2019.09.028" @default.
• W2974568513 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31542503" @default.
• W2974568513 hasPublicationYear "2019" @default.
• W2974568513 type Work @default.
• W2974568513 sameAs 2974568513 @default.
• W2974568513 citedByCount "27" @default.
• W2974568513 countsByYear W29745685132020 @default.
• W2974568513 countsByYear W29745685132021 @default.
• W2974568513 countsByYear W29745685132022 @default.
• W2974568513 countsByYear W29745685132023 @default.
• W2974568513 crossrefType "journal-article" @default.
• W2974568513 hasAuthorship W2974568513A5017387288 @default.
• W2974568513 hasAuthorship W2974568513A5021289768 @default.
• W2974568513 hasAuthorship W2974568513A5028314308 @default.
• W2974568513 hasAuthorship W2974568513A5041862198 @default.
• W2974568513 hasAuthorship W2974568513A5046146264 @default.
• W2974568513 hasAuthorship W2974568513A5058246995 @default.
• W2974568513 hasAuthorship W2974568513A5062317501 @default.
• W2974568513 hasAuthorship W2974568513A5080204034 @default.
• W2974568513 hasConcept C108586683 @default.
• W2974568513 hasConcept C12554922 @default.
• W2974568513 hasConcept C136229726 @default.
• W2974568513 hasConcept C159985019 @default.
• W2974568513 hasConcept C171250308 @default.
• W2974568513 hasConcept C175369904 @default.
• W2974568513 hasConcept C188027245 @default.
• W2974568513 hasConcept C192562407 @default.
• W2974568513 hasConcept C198826908 @default.
• W2974568513 hasConcept C2781052500 @default.
• W2974568513 hasConcept C28328180 @default.

• next